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Culture and immunohistochemistry of Schwann cells Schwann cells that are derived from sciatic nerve of Sprague-Dauley rats are successfully isolated, cultured. The Schwann cells are transplanted into the hemisected adult rat spinal cord after purification and condense and enclosed by L I -antibody. Eight weeks later, the experiments of the S- 100 immunohistochemistry indicate that Schwann cells are still alive and array orderly relatively.

本实验的主要研究内容及结果如下： 1、雪旺细胞的培养及免疫组化染色利用新生S-D大鼠的坐骨神经成功地分离、培养获得了纯度较高的雪旺细胞，并将雪旺细胞移植进入大鼠脊髓损伤创面，饲养8周后经过S-100免疫组化ABC染色发现雪旺细胞在大鼠体内成活良好并成簇排列。

The hilling activing of CD3AK+ cells showed significantly higher to K562/VCR cells than to K562 cells, while there was no difference of cytotoxicity between K562 /HHT and K562 cells.

4CD3AK+细胞对K562/VCR细胞的杀伤活性强于对敏感细胞K562，其对K562/HHT细胞的杀伤活性与其对K562细胞的杀伤活性比较，差异无显著性。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

Likewise, there was a significant result in the case of investigating whether luteal cells in early stage was affected by hypoxia similarly to those in middle stage, given big difference in the development of cell physiology. In this experiment, luteinized granulosa cells were used to mimic the early stage luteal cells. And luteinized granulosa cells were also cultured under the normoxic conditon and the hypoxic condition individually.

另一方面，由於中期黄体细胞发育已臻成熟，其细胞生理与黄体发育初期已然有所差异，因此本研究亦应用黄体化粒性细胞模拟发育初期之黄体细胞，并培养於正常氧或缺氧条件下，添加PKA讯息传递路径活化剂（oLH或8-bromo-cAMP）测定孕酮生成与相关蛋白质之表现差异。

The myoblasts are the precursor of the maturated muscle cells, while the satellite cells are a kind of mononucleus cells which have the characteristics and function of the myoblasts. The satellite cells participate in the growth of skeletal muscle, the repair after injury of the muscle and provide nuclei to the maturated muscle for the normal alternation of cell nuclei. They are very important to the maintenance of the function of skeletal muscle.

成肌细胞为成熟肌细胞的前体细胞，而肌卫星细胞则是成熟骨骼肌组织中具有成肌细胞特征和功能的一类细胞，参与骨骼肌的生长、损伤后的修复以及为正常骨骼肌细胞核的更替提供细胞核，对骨骼肌正常功能状态的维持很重要。

Results The cells transfected with E1AF is remarkably more invasive than those of control group. The image analysis and PCNA expression of the tumor cells demonstrate that transfected cells are m ore actively proliferative than the untransfected cells.

结果 转染后细胞的侵袭能力较转染前明显增强，肿瘤细胞图像分析和PCNA的表达，均显示转染后的肿瘤细胞增殖明显活跃。

Results: There were normal appearances of the morphology, volume and microscopic demonstration of both right and left hepatic lobes in sham-operating control group. In HA+PV group, hepatic right lobes were coagulatively necrosed. There was necrosis of liver cells in each sub-groups, accompanying with a few apoptic cells in the 3h sub-group. Along with the time extending, necrotic cells were increasing, and apoptic cells were decreasing. Necrosis in right lobes was at the most at 72h group.

结果：假手术对照组肝左右叶形态、体积以及各种病理学检查均未见异常；HA+PV组术后肝右叶发生凝固性坏死，3h即可见点片状肝细胞坏死，内见少量凋亡细胞，随时间延长，凋亡细胞减少，坏死细胞增多，至72h坏死更加彻底：PV组术后24h肝右叶萎缩，电镜及TUNEL均显示3h组织内可见凋亡细胞，至24h凋亡细胞数达高峰，72h凋亡细胞减少，出现局灶性坏死。

The histopathologic changes included lymphocyte and monocyte infiltration,hyperplasia of synovial cells and small vessels,interstitial fibrosis,hyaline degeneration and cartilaginous metaplasia.The immunohistochemical observations showed that the high expressions of IL-1 and IL-6 were significantly different between the pathologic plicae and the control groups（P＜0.01）.The positive expressions of IL-1 and IL-6 were the synovial cells and monocyt-lymph cells in the pathologic synovial plicae.The positive expressions of MMP-1 and TIMP-1 have significant difference between the experiment and control group（P＜0.01,P＜0.05）.The expression of MMP-1 was positive in synovial lining cell,monocyte,fibroblast,endothelial cell in small vessel and chondrocyte.The TIMP-1 expression was detected in the synovial lining cells and a small quantity fibroblast.

结果 正常滑膜皱襞和病理性滑膜皱襞在滑膜细胞增生及小血管增生、间质纤维化及玻璃样变、软骨化生组织学改变方面，差异均有显著性（P＜0.01）；IL-1、IL-6在病理性滑膜皱襞内的增生滑膜细胞、单核及淋巴细胞和在正常滑膜皱襞内的表达差异均有显著性（P＜0.01）； MMP-1、TIMP-1在病理性滑膜皱襞和正常皱襞内的阳性表达，差异具有显著性（P＜0.01，P＜0.05），MMP-1在增生滑膜衬里层细胞、单核和纤维母细胞、血管内皮细胞和软骨化生的软骨细胞呈阳性表达；TIMP-1只在滑膜衬里层细胞和少量纤维母细胞有表达。

Morphological changes of abdominal aorta: The aorta intima was smooth and endothelium cells were continuous with small intervals between cells in blank control group,endothelium cells presented normal configuration without edema;while intima in model control group became thick and upheaved, part of endothelinm cells detached with widened intervals.

腹主动脉形态学变化观察结果，可见空白组腹主动脉内膜表面光滑，内皮细胞连续，细胞间隙较小，内皮细胞无水肿，形态正常；模型对照组腹主动脉内膜增厚、隆起，血管内皮细胞可见部分脱落，细胞间隙增宽，内皮细胞有水肿。

In order to discuss the harm of long-time surfing the internet to the human body, we extract 35 undergraduate in our university and carry on the net continuously experiment. At the same time, 23 people (male 15 person, female 8 person) have been sampled on the track inspection on routine blood index. We discovered that continual 56 hour surfers can c ause obvious blood corpuscle metabolism function disorder, There are radical ascensions concerning the absolute value of blood routine inter cells, the number of white blood cells, the volume and quantity of the blood platelet and so on. The average volume of the red blood cells reduces significantly. The distributing volume and width of the red blood cells increase correspondingly.

为了探讨长时间上网对人体的危害，我们抽取在校本科大学生35人进行连续上网实验，对其中的23人(男15人、女8人)进行了跟踪血常规检查，结果发现连续56小时的上网，导致受试者出现明显的血细胞代谢功能紊乱，血常规中间细胞绝对值、白细胞计数、血小板体积及数量等多项指标出现应激性升高，平均红细胞体积显著性降低，红细胞体积分布宽度百分比明显增高。

Now she was hungry and angry.She began to smoulder.

现在她又饿又气，她开始流露难以抑制的怒火。

You have placed our iniquities before You, Our secret sins in the light of Your presence.

诗90：8 你将我们的罪孽摆在你面前、将我们的隐恶摆在你面光之中。