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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

Results AQP1 is expressed at the apical and basolateral membrane of the microvascular endothelium; AQP3 was detected at basal cells of both the bronchiole epithelium and submucosal gland acinus; AQP4 is present in the basolateral membrane of columnar cells in bronchiole; while AQP5 is expressed in the apical membrane of type Ⅰ pneumocytes, and also at the apical of columnar cells of superficial epithelium and submucosal gland acinar cells.

结果本研究发现AQPs基因在羊肺中的表达分布与人相似,AQP1在肺内的毛细血管内皮细胞表达;AQP3在小支气管黏膜上皮的基底细胞的基侧膜表达,AQP4存在于小支气管黏膜上皮的柱状纤毛细胞的基侧膜;AQP5存在于Ⅰ型肺泡上皮细胞的顶质膜,存在于小支气管黏膜上皮柱状纤毛细胞,以及在气道黏膜下腺的腺细胞的顶质膜表达。

CIK cells can kill specific tumor cells without injury to unconverted cells, lymphoblast induced by agglutinin or normal cells.

CIK细胞选择性地杀伤肿瘤细胞,对未转化的细胞、凝集素诱导的淋巴母细胞以及正常细胞没有毒性。

Here Rajasekhar and Vemuri hit brought unitedly essays and reviews by concern body in every areas of halt radiophone research--tissue and office halt and ascendent cells, cancer halt cells, Hellenic brute pluripotent halt cells, and pluripotent halt cells derivative by thermonuclear reprogramming of grown somatic grown radiophone nuclei.

这里Rajasekhar和Vemuri已聚集了世界各国领导人的文章和评论的干细胞研究的所有领域-组织和器官干细胞和祖细胞,癌症干细胞,古典胚胎多能干细胞,多能干细胞,并导出了核重新规划成熟的成年体细胞的细胞核。

Mesangial cells might derive from mesenchymal cells in the metanephrogenic blastema. The expression of α-SMA in developing mesangial cells is a transient phenomenon associated with the maturation of mesangial cells.

系膜细胞可能来源于生后肾组织的间充质细胞,α-SMA的表达短暂与肾小体系膜细胞的发育成熟密切相关。

Epidermal cells near the wound dedifferentiate and proliferate forming blastema and/or migrate to the wound plane forming a pre-epidermis consisting of several layers of stem cells covering the wound plane, which then re-differentiates further forming fully developed epidermis. Cells in the parietal peritoneum are also induced by injury to dedifferentiate into stem cells which then proliferate and migrate along the parietal peritoneum to the wound place forming a pre-peritoneum which re-differentiates into fully developed parietal peritoneum.

伤口愈合包括伤口闭合、顶端表皮层及体腔上皮的新生:创伤后的前4 d,残腕顶端的肌肉组织向伤口处迁移并重排使伤口闭合;创伤附近的表皮层细胞发生脱分化并增殖后迁移到创伤面形成由数层干细胞组成的前表皮层,之后进一步分化形成表皮层;而体腔上皮细胞在创伤诱导下也脱分化并增殖,然后沿体腔上皮迁移到创伤处形成"前体腔上皮",经再分化形成新的体腔上皮。

AIM:Studies on totipotential stem cells using primordial germ cells have the same application prospect as embryonic stem cells, especially for the animals difficult to isolate embryonic stem cells from blastula.

目的:应用原始生殖细胞进行全能干细胞的研究具有与胚胎干细胞同样广阔的前景,尤其适合于难以从囊胚内细胞团中分离培养胚胎干细胞的动物。

E and TUNEL Besides,early apoptotic cells which were densely green yellowstained or displaying green yellow fragments,late apoptotic cells which were densely cardinal redstained or displaying cardinal red fragments, and cardinal red swollen necrotic cells could be found in the AO/EB doubly stained cells.

AO/EB双染色法还能够显示呈致密浓染的黄绿色染色或出现黄绿色碎片的早期凋亡细胞,呈致密浓染的鲜红色染色或出现鲜红色碎片的晚期凋亡细胞,以及呈鲜红色膨大状的坏死细胞。

Part Two: The effect of fruit extract on CRL-2606 production of PGE2 Effect of anthocyanin rich fruit extracts on endothelial cells'PGE2 production Epidemical experiment shows that phenolics in fruits and vegetables may prevent the occurrence of chronicle cardiovascular heart disease. To investigate the activity of fruit extracts on artery blood vessel cells, we chose CRL-2606 cell line, a normal iliac artery endothelia cells. Using indomethacin as control, we investigate the effect of blueberry extracts, blackcurrant extracts, chokeberry extracts, cyanidin3-glucoside, and malvidin3-glucoside on the PGE2 production of CRL-2606 cells.

第二部分水果提取物对人体细胞系CRL-2606产生PGE2的影响水果提取物对血管内皮细胞释放PGE2的影响流行病学调查表明,蔬菜水果中的多酚类物质具有预防慢性心血管疾病的作用,为研究水果提取物对血管细胞的作用,我们选择回肠动脉内皮细胞CRL-2606细胞系作为研究对象,以indomethacin为对照,研究了blueberry,blackcurrant,chokeberry提取物及cyanidin3-glucoside,和malvidin3-glucoside对血管内皮细胞释放PGE2的影响。

LITERATURE EVALUATION: The 32 literatures are about ESCs transcription regulation, the role of FoxD3 for embryonic development and maintaining the pluripotency of ESCs and the function of FoxD3 in other cells (myeloid cells, nervous crest cells and chromatophore cells).

FoxD3是叉头框(Forkheadbox,Fox)家族中的一个转录调控因子,它对胚胎上胚层及其衍生物的形成和建立多潜能胚胎干细胞系具有重要作用,并与控制胚胎发育和胚胎干细胞多能性的其他转录调控因子有一定的联系。

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文兴一贯秉承&严格管理、全员参与、质量第一、用户至上&的企业方针,遵循优质、高效和追求卓越的经营理念,从而实现在技术上、规模上、管理上处于同行领先地位。

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