查询词典 nutrient cells

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Neural stem cells have a strong self-renew mechanism and it can transform after a little break. Neural stem cells have a long term survival, which mean that it has more probability of wrong copy than mature cells. These cells are formed glioma stem cells in the end. The genes who adjust neural stem cells can express in glioma stem cells, which hold out glioma stem cells from neural stem cells. There is another presume that glioma stem cells come from differentiated cells. Through the gene break of these cells, they can obtain characteristics of stem cells, then form glioma stem cells.

神经干细胞具有很强的自我更新机制，获得较少突变即有可能恶性转化，而且干细胞存活时间较长，这意味着干细胞比成熟细胞发生细胞复制的错误几率更大，因外界环境的刺激而发生突变的机会更多，最终形成脑胶质瘤干细胞，同时调节神经干细胞增殖和自我更新的基因在脑胶质瘤的脑胶质瘤干细胞中也表达，这也是支持神经干细胞是脑胶质瘤干细胞来源的；也有推测认为它可能起源于已分化的细胞，由这些细胞突变发生去分化得来，并通过基因突变而获得了干细胞自我更新的特性，从而形成脑胶质瘤干细胞。

In recent years, the company has successfully developed some special production lines for manufacturing ordinary batteries, namely, D size, Size C, and Size AA. Now running in the workshop is another assembly line for carbon rod processing machinery. For the past decade, it has introduced most advanced manufacturing techniques and control techniques from abroad, and therefore it has managed to develop automated machines for producing alkaline batteries. The automatic assembly lines that it possesses are: Lines LR6 and LR03 for 200 cells per minute, 300 cells per minute, 400 cells per minute, 600 cells per minute and 1000 cells per minute, Lines LR20, LR14 and LR61 for 150 cells per minute and 300 cells per minute. Powder spraying conducting film, machines for negative electrode calamine cream, diaphragm machines with a speed of 30 cells per minute; negative electrode electric welding machines with a speed of 200-300 cells per minute; current collector assembling machines with a speed of 200-400 cells per minute; battery tray fillers with a speed of 200-400 cells per minute, insulating ring assembling machines with a speed of 300 cells per minute; electroscope machines, 4-cell furling machines with a speed of 300 cells per minute.

公司先后开发了普通电池生产线：一号、二号、五号电池流水线和普通碳棒加工机械，特别是近十年来公司吸收并消化了国外最先进的生产工艺、控制技术，研制开发了国内最先进的自动化碱性电池机械，有200只/分钟、300只/分钟、400只/分钟、600只/分钟、1000只/分钟的LR6、LR03电池自动化生产流水线；有150只/分钟、300只/分钟的LR20、LR14、LR61电池生产线；有正极拌粉系统喷导电膜设备、负极拌锌膏；有30只/分钟的卷隔膜套机，200-300只/分钟负极钉高速点焊机，200-400只/分钟的集电体高速组装机，200-400只/分钟的电池装盘机，300只/分钟的绝缘圈组装机，300只/分钟的验电机和四节缩机等。

The results indicated as follows:the total nutrient elements accumulation of various Slash Pine stands amounted to 1273.83-1615.88kg/hm2,of which stem occupied about 60%. The nutrient retention accounted for 111.46-133.39kg/hm2*a,which is higher than that of either Chinese fir or Masson pine plantation. The nutrient return equalled to 90.43-105.09kg/hm2*a. Nutrient elementsturnover period is long while cycling rate is lower. The net production per ton needs 13.19-14.81kg nutrient.

结果表明：湿地松林分中养分元素总积累量为1273.83-1615.88kg/hm2，树干约占60%，积累速率为111.46-133.39kg/hm2*a，高于杉木和马尾松，归还量仅有90.43-105.09kg/hm2*a，其周转期长，养分循环速率低，每生产1t有机物需要上述养分元素共13.19-14.81kg。

The core issue of nutrient control was focused and the dynamic studies on phosphorus pollution of the whole watershed were conduced: 1 to highlight the non-point source pollution control, which is the difficulties research subject of water resource protection; 2 to perfect the calculation method for phosphate input and output, providing important basis on decision making for nutrient control; 3 to establish database and water quality model, to predict the water quality response after different phosphorus control schemes realized; 4 to determine admissible phosphorus loading and control standard according to the quantitative relationship between the external force function (mainly the exotic nutrient salt concentration or loading) and internal nutrient state response, and to work out the effective and practical techniques for eutrophication control.

围绕营养盐控制的核心问题，进行全流域范围磷污染的动态研究：1）突出了目前我国水资源保护研究难点—非点源污染控制；2）完善了流域水库磷盐收支计算方法，为流域营养盐控制决策提供了重要依据；3）建立数据库和水质模型，预测了在实施不同磷削减控制方案后流域供水水质的响应情况；4）根据湖泊外力函数（主要是外来营养盐浓度或负荷）与湖库内营养状态响应间的定量关系，确定了磷允许负荷量及控制标准，提出了有效的富营养化控制实用技术。

In order to assess the change of triploid populus tomentoza pulp plantation long-term-site productivity, The paper studied on effects of aboveground litterfall, fine root turnover and wet dust precipitation in nutrient cycling of triploid populus tomentoza pulp plantations at different ages,namely 2a、4a、5a、6a.It studied influence of different factors on decomposition of leaf、tree bark and twig of triploid populus tomentoza to select the operations to accelerate the decomposition and nutrient release. Finally, it studied influence of different intercrops on plantation site productivity and the relationship of intercrops and triploid populus tomentoza to select suitable intercrops. The main results as follows:(1)The aboveground litterfall of triploid populus tomentoza increased along with age from 216.03±59.7gm~(-2) at 3a to 482.38±101.3gm~(-2) at 7a, The N returned by litterfall wasl8.38±2.46kg.hm~(-2)a~-121.63±2.25kg.hm~(-2a~-139.51±4.61kg.hm~(-2a~-138.89±4.89kg.hm~(-2a~(-1) at 3a、5a、6a、7a respectively. The P returned by litterfall was 5.80±0.62kg.hm~(-2)a~(-1)、8.16±0.94kg.hm~(-2)a~(-1), 11.31±1.33kg.hm~(-2)a~(-1)、11.76±1.37kg.hm~(-2)a~(-1) at 3a、5a、6a、7a respectively. The nutrient returned by fine root turnover increased along with age, too. The N returned by fine root turnover was 3.85±0.41kghm~(-2)a~(-1)、5.22±0.63kghm~(-2)a~(-1),7.62±0.89kghm~(-2)a~(-1),9.17±1.22kghm~(-2)a~(-1) at 3a、5a、6a、7a respectively. The P returned by fine root turnover was 0.73±0.07kghm~(-2)a~(-1)、1.69±0.09kghm~(-2) a~(-1)、1.92±0.31kghm~(-2)a~(-1)、1.96±0.21kghm~(-2)a~(-1) at 3a、5a、6a、7a respectively. The leaf was the principal pathway to return nutrient to soil among litterfall, fine root turnover and wet dust precipitation. The proportion of returned N by leaf was 74.84%、71.96%、78.58%、75.03% at 3a、5a、6a、7a respectively,The proportion of returned P by leaf was 85.93%、80.31%、83.04%、83.23% at 3a、5a、6a、7a respectively. Therefore, it is important to protect and utilize the leaf in order to maintenance and enhance the long-term-site productivity of triploid populus tomentoza pulp plantation.

本文采取时序研究法，以3a、5a、6a、7a共4个不同年龄的三倍体毛白杨纸浆林为对象，研究了地上凋落物、细根周转、湿沉降在林分N、P营养元素循环中的作用及不同年龄林分N、P营养元素循环的特征，以评价三倍体毛白杨纸浆林长期立地生产力的变化；采取网袋法研究了不同因素对落叶、树皮、树枝分解的影响，以确定加快其分解、促进养分释放的措施：同时研究了不同间作物对林地影响、林木与间作物之间关系，以选择能维持立地生产力的合适的间作物种类等内容，得到以下结论：(1)随着年龄的增加，三倍体毛白杨地上凋落物的数量从3a的216.03±59.7gm~(-2)增加到7a的482.38±101.3gm~(-2)，通过凋落物归还的N分别为：3a时为18.38±2.46kg.hm~(-2)a~(-1)，5a时为21.63±2.25kg.hm~(-2)a~(-1)，6a时为39.51±4.61kg.hm~(-2)a~(-1)，7a时为38.89±4.89kg.hm~(-2)a~(-1)，归还的P分别为：3a时为5.80±0.62kg.hm~(-2)a~(-1)，5a时为8.16±0.94kg.hm~(-2)a~(-1)，6a时为11.31±1.33kg.hm~(-2)a~(-1)，7a时为11.76±1.37kg.hm~(-2)a~-1随着年龄的增加，通过细根周转归还的养分也在增加，归还的N分别为：3a时3.85±0.41kghm~(-2a~(-1)，5a时5.22±0.63kghm~(-2)a~(-1)，6a时7.62±0.89kghm~(-2)a~(-1)，7a时9.17±1.22kghm~(-2)a~-1归还的P分别为：3a时0.73±0.07kghm~(-2a~(-1)，5a时1.69±0.09kghm~(-2)a~(-1)，6a时1.92±0.31kghm~(-2)a~(-1)，7a时1.96±0.21kghm~(-2)a~-1从地上凋落物、细根周转、湿沉降三种不同途径归还林地养分所占的比例来看，地上凋落物中的落叶是归还养分主要途径，年龄在3a、5a、6a、7a时，通过落叶归还的N所占比例分别为74.84%、71.96%、78.58%和75.03%，归还的P所占比例分别为85.93%、80.31%、83.04%和83.23%。

The content of soluble Dietary fiber in nutrient alfalfa and extraction rate were high. the content of insoluble Dietary fiber in poddind alfalfa and extraction purity are high.After extracting Dietary fiber, the content of rudimental starch in podding alfalfa is higher than the nutrient ,however the content of rudimental protein in podding alfalfa is lower than the nutrient.it bring falling the purity of insoluble Dietary fiber in nutrient alfalfa.

营养期苜蓿水溶性膳食纤维含量及提取率均较高；结荚期苜蓿非水溶性膳食纤维含量较高；提取膳食纤维后结荚期苜蓿淀粉残留量高于营养期，而蛋白质残留量低于营养期，营养期非水溶性膳食纤维纯度相对结荚期较低。

Results: The numbers of Bombesin positive pulmonary cells, the lamina propria S-l00 protein, neuron-specific enolase positive nerve fibers, IgE positive cells, mast cells and IgE positive mast cells significantly increased in bronchiectasis. The changes of pulmonary endocrine cells, nerve fibers and IgE positive cells were more significantly in hyperplastic BALT areas. The S-100 and NSE were found in lymphoid tissue and BALT. A close contact was found between mast cells and the S-100 positive nerve fibers. An IgE positive outer zone was found on MC surface. Mast cells and IgE positive cells were seen in the bronchial epithelium and alveolar septa.

结果：支气管扩张症中，支气管上皮蛙皮素阳性细胞、固有膜S-100蛋白和神经特异性烯醇化酶阳性神经纤维、IgE阳性细胞、MC和IgE阳性MC均显著增多，且在支气管相关淋巴组织增生的区域上述肺内分泌细胞、神经纤维和IgE阳性细胞增多尤为显著，S-100蛋白和NSE阳性神经纤维分布於弥散淋巴组织和BALT中，MC与S-100蛋白阳性神经纤维紧密接触，MC表面有IgE阳性环状带，MC和IgE阳性细胞出现在支气管上皮间和肺泡壁。

The rate of c-kit+ cells in the heart increased gradually from E10 with the highest level of 12.6±3.2% at E11, which was nearly similar to the fetal liver, except for E12 when the c-kit+ cells was 3.4±1.2% in the heart compared to the fetal liver with 11.6±4.1% c-kit+ cells. The rate of c-kit cells in the suspension cells from the heart at E11 maintained stable after 24h culture based in the stromal cells of the heart and AGM region with the same conceptus age, but the rate of c-kit cells obviously decreased in the condition of the stromal cells from the fetal liver. The c-kit cells of the three conditions were all at low level at 48h without significant difference.

通过流式检测c-kit+细胞我们发现，胚胎期心脏和胎肝c-kit+细胞比率几乎一致，E11时c-kit+细胞明显增多，分别高达(12.6±3.2)%和(9.6±2.8)%，但E12时心脏中c-kit+细胞明显减少，仅为(3.4±1.2)%，此时胎肝中c-kit+细胞为(11.6±4.1)%。E11的悬浮细胞与心脏内皮细胞和AGM区基质共培养24h时，c-kit+细胞数量保持稳定，但在胎肝基质细胞中c-kit+细胞数量明显减少，在共培养48h，三组中的c-kit+细胞均呈较低水平，组间没有显著性差异。

Compared with classical cytotoxic T lymphocytes , NK cells are superior in the following aspects: NK cells are among the first cell types to be activated after intracellular pathogen stimuli, the activity of NK cells peaked between 1 to 3 days, retained during the first 7-10 days, and the T cells response emerged only after 7 days when the NK cell responses begin to decline; though comprising of only 10％-15％ of peripheral lymphocytes, NK cells mobilize most of the cell populations in a strong and high level response to different invasions, and act as key factors at early defense before a specific immune response could mount, while only certain clones were involved in T cell response; NK cells kill virus-infected or malignant transformed cells without pre-sensitization and without restriction by major histocompatibility antigen, which is usually a mechanism of immune escape to T cell recognition by virus-infected or tumor cells, thus NK cell in complementary with T cells play crucial roles in tumor and virus eradication.

NK细胞以其强大的细胞毒活性为特征，与细胞毒性T细胞相比，NK细胞具有如下特点：NK细胞虽然只占外周血淋巴细胞的10-15％，但是对刺激性因素产生应答的过程十分迅速，一次可以调动大多数细胞共同参与，而不是几个克隆的活化，免疫应答的强度高；病毒感染或恶性转化细胞的一个主要特征是细胞表达的MHCⅠ类分子下降或消失，并借助这一机制逃避特异性T细胞应答的识别，NK细胞介导的杀伤活性无需抗原刺激，不受MHCⅠ分子表达的限制，从而与T细胞应答互为补充发挥免疫防御的功能；NK细胞的免疫活性可以被多种细胞因子上调，其中IFNα，IL-2，IL-12，IL-15和IL-18具备更强的作用；NK细胞具有强大的细胞因子分泌功能，对于启动T细胞特异性应答必不可少。

We are in a good position to meet your requirements.

我们完全可以满足你方的需求。

We are all pervade d with a sense of disaster.

我们普遍有大祸降临的感觉。