查询词典 nurse balloon

At that time,there was a balloon pricked by needle in my mind....

那时脑海里出现了被针刺破的气球。。。

He pricked the balloon and burst it.

他把气球扎爆了。

But after being suspended for an instant aloft,the balloon began to redescend, the gas escaping by the rent which it wasimpossible to repair.

但是，没一会儿工夫，气球又开始下降了，气体从裂缝中向外跑，这裂缝现在要补也没法补。

Objective: The purposes of the present study are as follows: 1 To study the effect of direct contact with VSMC on transdifferentiation of MSC; 2 To construct MSC of Dual-stable expression of AT2R gene medicated by tetracycline regulatable system.; 3 To investigate the effects of AT2R gene medicated by tetracycline regulatable system on neointimal hyperplasia in rat carotid arteries after balloon angioplasty.

本实验体外观察MSC与VSMC在直接接触培养下的分化情况，并以MSC为载体，通过体外细胞基因转染及筛选，获得双重稳定受到强力霉素调控表达AT2R基因的MSC，将此受到调控表达的AT2R基因导入大鼠颈动脉球囊损伤动物模型中，观察MSC细胞移植实现AT2R基因在体可调控表达在新生内膜中形成的作用及潜在意义。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Christopher Skelly, MD, assistant professor of vascular surgery at the University of Chicago Medical Center, and the study's lead author says, One of the drawbacks of balloon angioplasty to open blocked arteries and the use of stents to keep them open is that arteries sometimes experience aggravation from the procedure.

Chicago大学医学中心血管外科助理教授Christopher Skelly是此项研究的主要负责人，他说：利用气囊或是支架进行的血管成型术一般用于打开封闭的血管，但是它们可能会带来副作用，使病情加重。

As a response, this seemed inadequate to the immensity of the balloon, the suddenness of its appearance over the city; on the other hand, in the absence of hysteria or other societally induced anxiety, it must be judged a calm,"mature" one.

作为一种反应，这态度对于气球的庞大无比以及它在城市上空的突然出现，似乎很不合适；另一方面，那些没有患歇斯底里症或者其他社会性人为忧郁症的人则毅然断言，这种反应是冷静的、&成熟的&。

Objective To compare the effects of endoscopic sphincterotomy vs endoscopic papillary balloon dilation in the treatment of choledocholith and to explore the indications for these two techniques.

目的 比较内镜下十二指肠乳头切开术与十二指肠乳头球囊扩张术两种方法治疗胆总管结石的优点和缺点，探讨两种方法的适应证。

China'strade surplus with the EU rose 25 per cent in the first eight months ofthis year to 70bn ($103bn, 49bn), and some EU policymakers expect thesurplus to balloon to more than double that size, generating everlouder calls from politicians for restrictions on imports.

今年前8个月，中国对欧盟的贸易顺差增加了25%，达到700亿欧元（合1030亿美元）。欧盟的一些决策者预计，这一顺差还会继续扩大，达到上述规模的两倍多，促使政界人物更加大声地呼吁限制进口。

The Little Balloon took leave of the strawman and rice seedlings, coming to a hill where many cows were eating.

小气球向稻草人和秧苗说再见，来到一片小山坡，山坡草坪上有牛群在那儿吃草；小气球看到一只很可爱的小乳牛，还依偎著牛妈妈身边吸著奶，「哈罗，小牛！

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。