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Methods The nude mice tumors were formed by injection of human ovarian cancer cell line AO and AO/HSV1-tkc (AO cells carried with HSV1-tk gene which was constructed in China) subcutaneously, and then were transplanted to the omentum of nude mice. The filter-passing culture fluid of VPC/HSV1-tkc was injected daily into the peritoneum of the nude mice in the group of in vivo. Finally, all of the three groups of nude mice accepted the treatment of GCV.

先将人卵巢癌细胞AO及携有HSV1-tk基因的AO细胞(AO/HSV1-tkc)注射于裸鼠皮下,形成肿瘤后再移植于裸鼠网膜,并以病毒生产细胞(VPC/HSV1-tkc)的培养液对AO网膜移植瘤进行体内转染,然后以GCV对体外AO/HSV1-tkc转移的肿瘤及经体内转染的AO肿瘤进行治疗。

Slaves, candle, tied up, chain, slave, handcuffed , domination, fetish , violent games, cum face, cum shoot , outdoor sex, cosplay, schoolgirls, teachers, office ladies, doctors, nurse, special interests, peeping, sex show, adult toys, nude activities, nude game, nude photos, uniform and cosplay sex game, college girls, schoolgrils, office ladies, nurse, housewife, wife, peeping, dildo, miniskirt, underwear, bra , high heels, panties , adult toys, playmate, real member fucking show, nudist activities nude game, exhibitionists, body touching, expose yourself, outdoor sex.

制服诱惑、角色扮演:学生妹、女学生、女教师、OL、秘书、女医生、护士、人妻、主妇、家庭主妇。特殊性爱:假阳具、偷窥、互窥、真人表演、真人 show、成人玩具。裸体活动:裸露、全裸活动、裸体游戏、人体写真、四级人体摄影、抚摸、剥光猪、爱抚、户外性爱、四级电影欣赏、面具派对、自拍写真。狂野性爱:抚摸性器官、玩乳房、玩阳具、射精、饮精、颜射、口爆、肛交、吹箫、卜野、自渎、打飞机、自慰、脱衣表现、后庭。恋物游戏:迷你裙、校服裙、短裙、高跟鞋、内衣、内裤、胸围、避孕套、安全套。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗;(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力;(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性;(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡;(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型,应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况,明确其靶向性;(6)将荷瘤裸鼠分3组进行放射免疫治疗,分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

In Rh2+DDP group 1000uM cisplatine and 100uM gensenoside Rh2 were injected to nude mice by abdomen. Growth status of tumors were observed. Diameter of tumors were measured. After 60 days,〓Tc-MIBI were injected from vein of nude mice tail to perform SPECT exam and calculate the value R of the tumors. Later the nude mice were killed.

将A549DDP细胞和A549细胞分别种植于裸鼠皮下,种植A549细胞的裸鼠为A549组,种植A549DDP细胞的裸鼠分对照组、DDP组和Rh2+DDP组,每组4只裸鼠。A549组和对照组的裸鼠腹腔注射生理盐水,DDP组裸鼠腹腔注射低效浓度的顺铂;Rh2+DDP组裸鼠腹腔注射低效浓度的顺铂及无毒浓度的Rh2。

C.: 1 pack of 1 dozen creamlaid envelopes and feintruled notepaper, watermarked, now reduced by 3: some assorted Austrian-Hungarian coins: 2 coupons of the Royal and Privileged Hungarian Lottery: a lowpower magnifying glass: 2 erotic photocards showing a buccal coition between nude senorita (rere presentation, superior position) and nude torero (fore presentation, inferior position) b anal violation by male religious of female religious, purchased by post from Box 32, P.

分别为:胸围二十八英寸和二十九英寸半,上臂围九英寸和十英寸,下臂围八英寸半和九英寸,大腿十英寸和十二英寸,腿肚子十一英寸和十二英寸。

In contrast to neighbouring Switzerland, where the authorities have announced plans to fine the "objectionable behaviour" of nude hikers, two villages in the central German Harz mountain range are planning special walking routes for nude ramblers.

两个在德国中央哈茨山脉的村庄,特别为裸体漫步者规划出步行路线。相反,邻国瑞士当局已经宣布,计划对裸体远足"不良行为"罚款。

Leake believes that is socially more acceptability to show nude women than nude men.

Leake认为社会上更容易接受女性裸体而不是男性。

To overcome the shortcomings in the existing nude picture filtering systems and image recognition techniques, a feature vector-based nude picture detection algorithm was proposed.

针对现有色情敏感图像识别技术的缺陷,提出了基于特征向量的敏感图像识别技术。

In 《China Mansion》, scenes are collected from all the masters in the western art history such as Angres, Courbet, Monet, Gauguin, Klein, Jones, Boucher, Rembrandt,Rubens, David, Renoir etc.The scene is decorated with coarsely copied traditional Chinese furniture,cheap copies of the western small objects, the numerous figures are all modeled by the vulgar nude, strikingly raffish and vulgar, the "nude" and the "bed"are the only point of connection with the original paintings. This cross time and space"cultural exchange"is utterly absurd creating many uncertain concepts (Wang Qingsong's words), but it is the portrait of the current blind cultural exchange.

中国之家》集合了西方艺术史大师如安格尔,库尔贝,莫奈、高更、克莱因、琼斯、布歇、伦勃朗、鲁本斯、大卫特、雷诺阿等经典作品的场景,场景以粗糙仿制的中国古典家具布置,廉价仿制的外国小玩意儿装饰,场景中众多的人物全部以粗俗的裸体模特扮演,充满中国当下的艳俗气息,裸体和"床"是唯一保留的与西方古典原作的联系点,这种跨时空的"文化交流"十分荒诞,"产生了许多不确定的概念",但却是中国当下盲目的文化交流的写照。

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

体外培养的脂肪干细胞复合于支架上,加入诱导液(基础培养液+ 50 μg/L软骨形态发生蛋白1)继续培养2周作为实验组;将SD仔鼠软骨细胞复合于支架上,常规培养2周作为对照组。20只裸小鼠,左侧腋窝皮下均植入实验组细胞-支架复合物,右侧腋窝皮下均植入对照组细胞-支架复合物。8, 12周各处死10只,行甲苯胺蓝和番红O-固绿染色。

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The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他,我不喜欢他的这种性格。

As we know the price of traditional product is composed of the cost and the profit of the company involving market competition, monopolizes and many other factors.

我们知道作为传统的商品,定价的模式往往是在成本的基础上增加厂商的预计利润而形成其价格,当然也要考虑到市场竞争、垄断等其他方面的因素。