查询词典 nucleotide

Two 15nt complementary ssDNA primers were ligated into double stranded satRNA.Complete sequence of satRNA was obtained by RT-PCR using these 15nt ssDNA as amplification primers.Phylogenetic analysis showed that the identity of this 383nt satellite and some documented satRNAs was 72.6 to 99.5% at the nucleotide level.

以常规引物对感病植物的总RNA进行RT-PCR扩增，获得CMV RNA3全长克隆，经测序显示该RNA3序列属于CMV亚组Ⅱ；通过在卫星dsRNA两端分别加上15nt的单链DNA接头，以接头互补序列为引物进行扩增获得一个383nt的卫星RNA的全长序列。

Objective To clone the EG95 gene of Echinococcus granulosus in Inner Mongolia and analyze the gene nucleotide sequence.

作者：李志伟作者单位：内蒙古大学生命科学学院，呼和浩特 010021

Methods Human hepatocarcinom cells lines were transfected with an ectogenesis p53 (Adp53) to observe the action of p53 on wnt signaling pathway by fluorescence-activated cell sorting and RT-PCR.

利用反义寡核苷酸(oligodeoxy nucleotide，ODN)将p53反义ODN导入到人肝癌HepG2(含野生型p53)和人大肠癌Lovo(含野生型p53)细胞株中，观察p53表达受阻后对Wnt通路及FrpHE、DKK-1表达的影响，以进一步明确p53的调节作用。

The PAL-1 gene promoter defined the nucleotide sequences CTCCAACAACCCCTTC responded to both UV irradiation and elicitor application.

在PAL基因中的类似区域有一个17个核苷酸组成的区域elre参与植物对紫外照射和激发子应答。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根据Bt杀虫蛋白Cry1A和豇豆胰蛋白酶抑制剂的高级结构、杀虫机理及昆虫体内肠激酶的作用机理，本着尽量不影响二者功能的原则，设计出了构建Cry1A和CpTI融合杀虫基因CryCI的方案，即将CpTI基因的编码序列连接到GFM Cry1A基因编码区3'端，并在其间用一段编码肠激酶切割位点的序列相连。

RESULTS:①Enzymolysis and nucleotide sequence analysis of recombinant plasmids showed that two VEGF-C siRNA eukaryotic expression vectors were constructed successfully.

结果：①重组质粒的酶切、核苷酸序列分析结果：经酶切和测序鉴定证实成功构建了血管内皮生长因子C小干扰RNA表达载体，建立了稳定转染细胞株。

The homology of the nucleotide sequences and genetic variation analysis of NDV strains suggested that mere were obvious divergence in gene lever among NDV vaccine strains and epizootic strains isolated from different areas and different ages.The divergence may have effect on amino acid and structure and function of protein in some extent and lead to difference of immunogenicity ,which was one of reason being difficult in preventing from ND.

通过对NDV F基因核苷酸序列同源性比较及遗传变异分析发现：我国不同年代、不同地区分离的NDV毒株与疫苗毒株之间在基因水平上存在较大差异，这种差异对氨基酸及蛋白质的结构和功能可能会有一定影响，而NDVF蛋白的差异同时也造成免疫原性的差异，这很可能是新城疫的免疫防治十分困难的原因之一。

The novel primers were evaluated by detecting BTV serotypes 1, 3, 5, 8, 10, 11, 21 and 22. The specificity of the primers was estimated by comparing to gene sequences of viruses published in GenBank, and further assessed by detecting BTV serotype 1-12 and Epizootic hemorrhagic disease virus serotype 1-4. The sensitivity and repeatability of PCR with the novel primers were evaluated by successfully detecting the recombinant plasmid pGEM-T121 containing the diagnosed nucleotide sequence.

Blast软件分析引物与GenBank发表的病毒基因的同源性，显示引物特异性较好；利用8个不同血清型BTV标准毒株进行RT-PCR检测，证实该引物能有效检测不同血清型BTV；通过BTV1-12和EHDV1-4进行检测，证实该引物具有较好的检测特异性；通过制备的含特检序列的质粒标准品P121进行检测，显示该引物检测的敏感性为105 copies，重复检测CV%为0；对模拟血清样品进行检测，结果均为阳性。

With the direction of Professor Zhang Jiawei, basing on the clinic research of treating falling sickness with thread embedding therapy, summing-up many years clinic experience of thread embedding therapy, we observe and compare the two curative effect of thread embedding therapy and general acupuncture in treating chronic gastritis, in addition we study corresponding functionary mechanism with stomach electricity graph、gastrin、nucleotide、SP etc and provide science basis and theory instruction. According to the criterion of diagnosis and elimination set, 70 patients are took into the trial whom come from No. 1 affiliated hospital, among them 36 case are in the thread embedding Group, 34 case are in the acupunture Group.

本课题是在导师张家维教授及李素荷教授等协助导师共同指导下，在既往穴位埋线疗法治疗癫痫临床研究基础上，总结多年来穴位埋线疗法的临床经验，对穴位埋线及穴位针刺两种方法治疗慢性胃炎的疗效进行观察与比较，并拟用胃电图、脑肠肽、环核苷酸、P物质等指标变化来探讨其中的可能治疗机理，为临床的应用及推广提供科学的依据与理论指导。

Genetic diversity and phylogenetic relationship in goldfish were examined using mitochondrial D-Loop partial sequences at eight representative varieties of goldfishes and wild crucian carp. The eight representative varieties were Red Common Goldfish, Red Fantail, White Oranda with Red Carp, White Pearlscale, Red Tigerhead, Black Moor, Red Moor with Butterfly Tail and Red Bubble-eye with Dorsal Fin. 699 base-pair nucleotide sequences of Mitochondrial D-Loop were examined and analyzed for genetic polymorphism, Sequences data showed that all 129 sequences were grouped into 17 haplotypes. Nine representative varieties shared same haplotype. Five representative varieties of goldfishes had haplotypes respectively.

采用PCR技术扩增了红草金鱼，红文鱼，红顶白高头，白珍珠，红狮头，红龙睛蝶尾，墨龙睛，红水泡八个品种金鱼和野生鲫线粒体DNA的D-Loop区部分序列，PCR产物经纯化、测序、同源序列比对后获得长度为699bp的核苷酸序列，在129个样本中共检测到了17个单倍型，9个品种共享1种单倍型，5个品种的金鱼(BM， MB， WR， RF， RC)检测到各自独有的单倍型。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。