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To optimize this artificial transcription factor, a nucleotide sequence coding for 12 glycins was introduced between GAL4 DBD and HIF-1α CAD and ODD domains by PCR. Glycin arm could separate these 2 domains and render them enough space to fold and exert better function.

为优化这一融合蛋白，在GAL4 DBD和HIF-1α转录激活结构域之间利用PCR方法引进一段编码12个甘氨酸的序列，试图通过甘氨酸臂使两个不同的结构域分别获得更有利表现活性的空间结构。

The nucleotide sequence and the predicted protein sequence shared high sequence identity with other mammalian homologues.

比较猪RPLP0基因和人及小鼠同源基因的cDNA序列和蛋白质序列，结果表明该基因在3个物种中具有高的相似性。

In nucleotide level, both the coding sequence and 5' intron sequence are very conservative.

在核苷酸水平上，不但编码序列具有较高的保守性，而且内含子的5'端也是非常保守的。

In this study, PCR-RFLP and PCR-SSCP analysis were commanded on the Intron 17 and 18 nucleotide sequences of KIT gene.

通过利用PCR-RFLP和PCR-SSCP技术对中国地方猪种KIT基因内含子17、18的序列进行多态性分析。

The single nucleotide polymorphism of growth hormone gene was investigated in various breeds of duck, including Beijing ducks, Xihu mallards, Jinding ducks, Shan Partridge ducks, Jingjiang ducks and Shaoxing ducks. The primers for intron 2 and 3 in GH gene were designed based on the database of duck genomic sequence and the SNPs were detected by PCR-SSCP method.

根据鸭生长激素基因内含子2、3的序列设计5对引物，利用PCR-SSCP方法对北京鸭、西湖野鸭、金定鸭、山麻鸭、荆江鸭、绍兴鸭等6个鸭品种进行了单核苷酸多态性分析，并检测其多态性。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

Methos: The content of organophosphate in nucleotide has been as a target. It was determined by molybdenum blue spectrophotometry, with ascorbic acid as the reductant.

采用抗坏血酸-钼蓝显色定磷法，检测核苷酸中有机磷的含量。

Methos: The content of organophosphate in nucleotide has been as a target. It was determined by molybdenum blue spectrophotometry, with ascorbic acid as the reductant.

采用抗坏血酸－钼篮显色定磷法、检测核苷酸中有机磷的含量。

The effects of miosis and IOP lowering of Erycibele alkaloid are all mediated by M3 receptor subtype and its signal transductive mechanism is connected with cyclic nucleotide system.

丁公藤碱的缩瞳和降眼压作用主要通过M3受体介导，其信号转导机制与环核苷酸系统相偶联。

The reversible genes were particularly involved in xenobiotic functions (managing chemicals not produced in the body), nucleotide metabolism and mucus secretion.

可逆基因一般和异生物质功能（管理不由身体产生的化学物质）、核苷代谢、黏液分泌有关。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。