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In order to study species resource from ruminal fluid, one strain of methionine-degrading bacterium MB6-1 was isolated and purified. 16 S rRNA encoding gene (16 S rDN4) from MB6-1 was amplified by PCR, and its nucleotide sequence was determined.

为分析研究山羊瘤胃液中甲硫氨酸降解菌群的物种资源，对经分离纯化获得的一株甲硫氨酸降解菌MB6-1，采用PCR方法扩增其16 S rDNA基因，并测定其基因的核苷酸全序列。

In order to study species resource from ruminal fluid, one strain of methionine- degrading bacterium MB6-1 was isolated and purified. 16 S rRNA encoding gene (16 S rDNA) from MB6-1 was amplified by PCR, and its nucleotide sequence was determined.

摘 要：为研究山羊瘤胃液中甲硫氨酸降解菌群的物种资源，对经分离纯化获得的一株甲硫氨酸降解菌MB6-1，采用PCR方法扩增其16 S rDNA基因，并测定其基因的核苷酸全序列。

Compared with Sacbrood virus, the nuc leotide sequence of the fragment was 86.8%identical and the amino-acid sequen ce deduced from the nucleotide sequence shared 93.4%identity.

序列分 析表明，该片段为中蜂囊状幼虫病病毒部分结构蛋白基因，与意蜂幼虫囊状病病毒结构蛋白基因序列的同源性为86.8%，与之对应氨基酸序列的同源性高达93.4%。

Shandong sinder technology limited company,qingdao 266061,shandong,chinaabstract:to provide the eukaryotic expression vector carrying 6x histidine-tag and human growth hormone in expremental animal and cell culture,according to the published nucleotide sequence of the lactofericin b and human growth hormone,synthesis sequence was ligated with pmt-18 vector.

以牛乳素为目的基因，构建含有组氨酸标签、人生长激素信号肽的真核表达载体，为利用牛乳素在活体动物及培养细胞中的表达提供基因材料。

The StLTPal gene is well conserved in the coding region with 60-92% identity at the amino acid level, and 75-85% identity at the nucleotide sequence level compared with other Solanaceae nsLTPs.

基于氨基酸序列构建的系统发生树揭示，该基因与其他茄科nsLTP具有高度保守的序列相似性，核酸和氨基酸水平分别具75%~85%和60%~92%的同源性。

SsAPX showed the highest homology to the APX gene of Spinacia oleracea, with 84% identity in nucleotide sequence and 89% identity in deduced amino acid sequence.

BLAST同源性分析表明，该cDNA与已报告的菠菜细胞质抗坏血酸过氧化物酶基因同源性最高，在核苷酸水平上一致性为 87%，在氨基酸水平上一致性为 89%。

Professor Marcelin: I think this is a good idea because we know the nucleoside and nucleotide analogs are sublimitation and also you got your own limitations.

Marcelin教授：我认为这是个好办法，因为我们知道核苷类药物是次限制的，而每个人会有自己的限制。

A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein gene of TAV from England.

将其克隆到 pGEM Teasy中，经酶切和序列分析表明所克隆的是TAVCP基因，与已知的 6个株系相应序列同源性均在 96 。4%以上

The virus had serological relationship with IgG of TAV. A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein gene of TAV from England. The expected size of 886 bp was amplified by RT- PCR.

根据英国报道的番茄不孕病毒CP基因序列，设计合成一对引物，对该病毒RNA进行RT PCR扩增，得到与预期大小相符的特异扩增带。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。