查询词典 nucleotide

The identities of the pGM-CSF coding sequence when compared to human, bovine, ovine and murine sequences were 82%, 85%,88% and 86%, respectively at the nucleotide level, and 72%, 73%, 73% and 57%, respectively at the amino acid level.

与人、牛、羊、小鼠的 GM-CSF 核苷酸序列的相似性分别为 82%、 85%、 88%和 86%，氨基酸序列相似性分别为 72%、 73%、 73%和 57%。

To establish a method to differentiate Muscovy duck parvovirus from goose parvovirus, primers GPVF/R1 and MDPVF1/R1 were designed according to the genomic sequences of GPV and MDPV to amplify the virus culture and nucleotide of GPV, MDPV, duck plague virus, duck hepatitis virus, duck reovirus, canine parvovirus and feline panleucopenia virus.

根据番鸭细小病毒和鹅细小病毒基因组的非同源序列各设计了1对引物MDPVF1/R1和GPVF1/R1，建立了一种PCR方法，用该PCR方法分别对GPV、MDPV、鸭瘟病毒、鸭肝炎病毒、鸭呼肠孤病毒、犬细小病毒和猫泛白细胞减少症病毒的病毒培养物及其核酸进行扩增。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

The nucleotide acid sequences of the four fragments had more than 95% homology with Lectin gene (AF001527) and Pectate Lyase gene (x92943) which were submitted to GenBank .

此四条片段的核苷酸序列分别与GenBank中发表的Lectin基因(AF001527)及Pectate Lyase基因(X92943)具有95％以上的同源性。

Complete nucleotide sequence of genome of porcine reproductive and respiratory syndrome virusisolate was first reported in this paper in China.

在国内首次完成了猪繁殖与呼吸综合征病毒分离毒株的全基因组序列测定。

The relationship between the genetic deficiencies and hemorrhagic disorder were characterized. The homozygosity of the Thr359Met in the propositus with FⅦ: C activity 2% was related to severe clinical symptoms; The proband with double heterozygous lesions of Arg152Leu and one single nucleotide deletion at position 11487-9, combined with Arg304Trp in exon 8 with FⅦ activity 1% suffered severe clinical bleeding tendency. one proband with double heterozygous mutations (Agr304Trp and Arg304Gln) with 10% of FⅦ activities characterized asymptom. the heterozygous mutations (Thr359Met, Arg152Gln,-55C→T) with FⅦ: C 1%～5. 5% showed moderate or mild clinical symptoms.

其中8961 G＞T（Arg152Leu）和10966-8delC两种突变为国际首次报道，IFSla+5g＞a突变导致的异常剪接为国际首次报道。1个F7及F10基因联合缺陷家系，其中，F10基因28139 G＞T（Val384Phe）突变为国际首次报道；发现了1个由F7及组织因子(tissue factor，TF)两种基因联合缺陷导致的出血家系，其突变分别为F7基因启动子区-55C＞T杂合突变；TF 9363 C＞T（Arg131Trp）杂合多态性（频率2.63％）。6种F7基因突变发生在催化区；2种突变发生在裂解位点；1种发生在启动子区；1种突变发生在剪接位点，除一种缺失突变外，其余均为点突变；所有家系的基因突变都来自先证者的父亲和/或母亲。

Pseudorabies virus ; Glycoprotein G ; Nucleotide sequence ; Amino acid sequence ; Gene expressing

伪狂犬病病毒；糖蛋白G ；核苷酸序列；氨基酸序列；基因表达

Purine derivatives are a kind of important semi-pharmic of nucleotide.

嘌呤衍生物是一类很重要的药物中间体。

Results Morphine and purine nucleotide interventions improved the inhibition of morphine on PC12 cell proliferation.

结果 同时给予吗啡和嘌呤核苷酸可改善吗啡对PC12细胞增殖的抑制作用。

To investigate the effect of exogenous purine nucleotides on neuron nucleotide anablism depression.

目的：探讨外源性嘌呤核苷酸对吗啡致神经细胞核苷酸合成代谢降低的影响。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。