查询词典 nucleolus

In this paper,we used hexamethylene bisacetamide as a inducer to induce human mucoepidermoid carcinoma cell line MEC-1 cells to differentiate in vitro and vivo.The nucleolus organizer regions was investigated by means of the argyrophil technique.

本实验以六亚甲基二乙酰胺作为分化诱导剂，对涎腺粘液表皮样癌MEC-1细胞进行了体内外诱导，并通过银染技术对对照组和HMBA诱导组癌细胞核内核仁组成区的数目、大小及分布特征进行了观察。

Results In the medium and high dose F0 groups, it was observed that the atrophy and incrassation of seminiferous tubule, decrease of spermatogenesis, hyperplasia of interstitial tissue, especially in high dose groups spermatozoon abnormality and nucleolus concentration in the rats testis after DU ingestion for 14 months.

结论长期摄入贫铀对大鼠组织脏器的损伤具有一定的时间性和组织特异性，主要损伤集中在雄性生殖系统、免疫系统和造血系统，而对其他组织的损伤不明显。

The AO staining indicated that the cells had slippery verges and inerratic nucleolus.

AO染色鉴定显示细胞边缘平整，细胞核小且形态完整，符合正常细胞的形态。

Water stress could largely affected on cell structure of leaf, with enhanced degree of water stress, the karyon amount decreased, and the nucleolus reduced, distorted or degraded.

水分胁迫对叶细胞结构也有很大影响，随着水分胁迫程度的增强，细胞核数量减少，核缩小、变形或降解，解除胁迫以后也很难恢复。

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

Compared with the corresponding BEAS-2B cells, the shape of cell clone, the way of cell growth, the shape and quantity of cell and nuclear were not significantly changed in the 1st, 2nd, 5th passage BEAS-2BNNK cells. The shapes of cells and its nuclears of the 10th, 15th, 20th, 25th passage BEAS-2BNNK cells became round gradually, and the way of cell growth became from radio-form into membraniform and more than one nucleolus appeared.

BEAS-2BNNK细胞在第1、2、5代时细胞克隆形态及生长方式、细胞形态、细胞核形态及数目与相应代BEAS-2B无明显差异；第10、15、20、25代时细胞及胞核逐渐变圆，出现多个核仁，细胞由放射状生长逐渐变为膜状生长。

Volume of cell ill will color is larger, polygonal, afterbirth film is clear, afterbirth qualitative translucence is fine meshy, afterbirth nucleus shrinkage, visible nucleus channel and nuclear different, nucleolus is unidentified show; and acidity of qualitative be addicted to of afterbirth of acerbity cell of be addicted to submits fine grained form, apparent nuclear week sky is dizzy it is thus clear that.

嫌色细胞体积较大，多角形，胞膜清楚，胞质半透明细网状，胞核皱缩，可见核沟及核异型，核仁不明显；而嗜酸细胞胞质嗜酸性呈细颗粒状，可见明显的核周空晕。

The epidermis had thick cytoplasm, distinct nucleolus and lots of organelles. Cerasus tomentosa was the special in the experimental plants no matter appearance or inside structure, and the others also had some differences in the cells number of epidermis and nectariferous cell.

实验中所用的几种樱属植物花外蜜腺中毛樱桃无论从外观还是内部结构都与其它几种相差较大，而其它几种之间在分泌表皮细胞和泌蜜组织细胞的层数上也存在差异。

Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果：细胞接触肽段后存活率下降28.9％；光镜下可见细胞贴壁不良，胞体缩小，细胞突起断裂缩短；荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞；电镜下可见胞质中出现空泡样结构，细胞染色质浓集于核膜内侧并裂解成碎块状；流式细胞仪检测细胞出现亚二倍体峰，DNA电泳出现梯状带。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷