查询词典 nucleic

Use dynamic programming algorithm for nucleic acid sequence of February 2 ratio on the source code.

利用动态规划算法进行核酸序列的两两比对的源代码。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Transfection particles for delivery of nucleic acid into higher eucaryotic cells in vitro and in vivo comprises one or more nucleic acid molecules condensed by organic cationic molecules.

用于在体内和体外传送核酸至高级真核生物细胞内的转染颗粒，包括一个或多个由有机阳离子分子凝聚的核酸分子。

In the fourth chapter , we studied the resonance light scattering spectral characteristic of patent blueⅤ- Cetyltrimethyl ammonium bromide-nucleic acid system, and the elementary reaction mechanism with absorption spectra method and 1H NMR spectra method. Another new determination method of nucleic acid at nanogram level was established.

第四章研究了五号专利兰-十六烷基三甲基溴化铵-核酸体系的共振散射光谱特征，并结合吸收光谱法、核磁共振光谱和一系列条件实验探讨了它们的作用机理，建立了一种测定纳克级核酸的新方法。

CTAB was used to pyrolyse plant cell and reacted with the released cell nucleic acid to form nucleic acid-CTAB complex, the cell extract was then deproteined with chloroform-isoamylol, at last RNA was selectively precipitated by LiCl.

采用CTAB裂解植物材料，与细胞中的核酸形成核酸-CTAB复合体，然后用氯仿一异戊醇去除细胞裂解液中的蛋白，最后采用LiCl选择性沉淀其中的RNA。

CTAB was used to pyrolyse plant cell and reacted with the released cell nucleic acid to form nucleic acid-CTAB complex,the cell extract was then deproteined with chloroform-isoamylol,at last RNA was selectively precipitated by LiCl.

但是一般高等植物组织内都含有多糖类物质，这些物质的存在严重影响RNA的提取，如果去除不净，将影响反转录、扩增等下游操作。

The content of protein and nucleic acid in winter wheat leaf in its different growth stages were measured after the treatments of different water in order to vertically and horizontally research the metabolism of protein and nucleic acid in leaf in the time and spatial level.

［目的］在冬小麦不同生长发育阶段测定不同水分处理的叶片蛋白质和核酸含量，对叶片蛋白质、核酸代谢从时间、空间上进行讨论。

Gold nucleic acid drinks poured force one listed on the good taste, easy to absorb, the effect of the exact characteristics of the broad masses of customers, especially by middle-aged and elderly of all ages. In 2004, the Company also launched the "throwing power gold nucleic acid drinks," the replacement Product --- throwing force oral liquid gold.

泼力金核酸饮品一上市，就以口感好、易吸收、效果确切等特点，受到广大顾客尤其是中老年人的青睐。2004年，本公司又推出了&泼力金核酸饮品&的换代产品---泼力金口服液。

Peptide nucleic acid is a kind of new nucleic acid mimic invented in the early 1990s', in which the deoxyribose phosphate backbone has been replaced by a pseudo-peptide polymer to which the nucleobases are linked. PNA is uncharged, highly stable in chemical and biological environments, and binds complementary DNA and RNA targets with remarkably high affirnity and specificity.

肽核酸是二十世纪九十年代初合成的一种全新的核酸类似物，它以多肽骨架完全替代DNA中的脱氧核糖磷酸二酯键的骨架，呈电中性，具有极高的生物和化学稳定性、极强的核酸结合能力和特异性，在分子生物学的许多方面得到广泛应用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。