查询词典 nucleic

There were 2 peaks of starch accumulation in the process of somatic embryogenesis,the first time was at the stage of embryogenic callus,the second time was at the stage of globular embryoids;and a small raise appeared at the stage of torpedo-shaped embryoids,and the content of starch was slightly higher than those of heart-shaped embryoids and cotyledonary embryoids.With the development of embryos,the tendency of the changes of the content of DNA,RNA and the total nucleic acids was approximately similar,and the peak values were all at the stage of cotyledonary embryoids,and the change of the content of RNA was more remarkable than those of DNA and the total nucleic acids.The tendency of change of the content of proteins almost took on the S-shaped curve.

结果表明：龙眼胚性愈伤组织体胚发生过程中淀粉有2个累积峰值，第1次出现在胚性愈伤组织阶段，第2次出现在球形胚阶段，在鱼雷形胚阶段又有一个小回升，这时淀粉积累较心形胚和子叶形胚阶段高一些；随着胚胎的发育，DNA、RNA、总核酸含量的变化趋势大致相同，最高点均出现在心形胚阶段，其中RNA含量的变化较DNA、总核酸含量明显，蛋白质含量的变化曲线呈&S&形。

Carbohydrates are made of simple sugars, proteins are made up of 20 amino acids, and nucleic acids are compose of oxy- and deoxy- nucleic sugars and 5 base pairs.

其中碳水化合物都是由单糖构成的，蛋白质是由20种氨基酸构成的，核酸是由核糖、脱氧核糖、和5种碱基构成。

In part one, we prepared three ramification of pyrene nanoparticles by reprecipitation method under ultrasonic radiationand characterized with TEM.The determination of organic nanoparticles to DNA, NO2 -,and nucleic acids werestudied by using fluorescence. And some ultrasensitive determination of DNA, NO_2~-, and nucleic acids methods were proposed. The possible mechanism has been studied.

第一部分在超声辐射下用再沉淀的方法制备了几种芘的衍生物的纳米粒子荧光探针，并通过透射电镜进行了表征，并通过荧光法研究了它们分别与蛋白质、核酸以及NO_2~–的作用，建立了超灵敏的检测上述物质的方法，探讨了作用机理，并提出了合理的解释。

Based on the quenching of resonance light scattering of colloidal silver chlorid e by nucleic acids, has been developed for the determination of nucleic acids in aqueous solutions.

报道了一种测定水溶液中核酸的方法，该法基于核酸对氯化银溶胶共振散射光的猝灭作用。

In the chemistry research field of nucleic acid, it has been very active that the small molecules is used as anticancer drugs, the dye of nucleic acid and construction probe.

在核酸的化学研究领域中，小分子用作癌化学治疗剂、核酸染色剂及结构探针等一直是非常活跃的研究方向。

Experimental results show that there are existing some indifferent mechanisms to the pollen sterilization of the Ms types: during the period of single nucleic to three nucleic pollen cell,we observed that the cell of xylem and phloem in anther septum abnormally developed,also the cell in the sheath of vascular bundle,smaller and arranged irregularly; the metabolic block developed too,es...

结果表明，不育类型的花粉败育有着一些共同的机理：在花粉单核期至三核期可见到花药隔维管束的木质部及韧皮部细胞发育较差，维管束鞘细胞发育不良，体积较小，排列紊乱；在单核花粉期至三核花粉期均发生物质代谢障碍，特别在花药部位尤为明显；由于维管束发育不良和花药物质代谢障碍，导致了花粉缺乏营养而败育。

Phages usually have complex capsids composed of a polyhedral head, containing the nucleic acid, and a helical tail, through which nucleic acid is injected into the host.

噬菌体通常由复杂的衣壳组成一个多角体的头部，包含有核酸（ DNA 或 RNA ）和螺旋状的尾管，通过这个尾管核酸可以被注入寄主的体内。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Transfection particles for delivery of nucleic acid into higher eucaryotic cells in vitro and in vivo comprises one or more nucleic acid molecules condensed by organic cationic molecules.

用于在体内和体外传送核酸至高级真核生物细胞内的转染颗粒，包括一个或多个由有机阳离子分子凝聚的核酸分子。

Article 144 The Government of the Hong Kong Special Administrative Region shall maintain the policy previously practised in Hong Kong in respect of subventions for non-governmental organizations in fields such as education, medicine and health, culture, art, recreation, sports, social welfare and social

第一百四十四条香港特别行政区政府保持原在香港实行的对教育、医疗卫生、文化、艺术、康乐、体育、社会福利、社会工作等方面的民间团体机构的资助政策。原在香港各资助机构任职的人员均可根据原有制度继续受聘。

Small wonder, then, that the Chinese spend more in the shop than any other group of foreign visitors do .

这样的小惊喜，使中国顾客比任何国家的人消费得更多。