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The method involves exposing the cells to a compound having the formula I in which: w is a nucleic acid x is a non-amino acid or non-peptide nucleic acid binding group y is a spacer having a chain length equivalent to 1-30 carbon-carbon single covalent bonds or is absent R4 is H or halogen or CH2O-R3; and R1, R2 and R3 are the same or different and are either hydrogen, methyl, ethyl, alkyl, alkenyl, hydroxylated alkyl, hydroxylated alkenyl groups or ether containing alkyl, alkenyl, hydroxylated alkyl or hydroxylated alkenyl groups optionally being an acyl group having a carbon chain length equivalent to 3-24 carbon atoms saturated or unsaturated, with the proviso that at least one of R1, R2 or R3 includes a group having a carbon chain of 3-24 carbon atoms saturated or unsaturated, or to a compound having the formula II in which: w is a nucleic acid x is a non-amino acid or non-peptide nucleic acid binding group y is a space having a chain length equivalent to 1-30 carbon-carbon single covalent bonds or is absent, R5 is alkyl, alkenyl, hydroxylated alkyl, hydroxylated alkenyl group or ether containing alkyl, alkenyl, hydroxylated alkyl or hydroxylated alkenyl group optionally being an acyl group having a carbon chain length equivalent to 3-24 carbon atoms saturated or unsaturated, with the proviso that R5 includes a group having a carbon chain of 3-24 carbon atoms saturated or unsaturated.

该方法包括使细胞暴露于具有式的化合物，在式中：w是核酸，x是非氨基酸或者非肽核酸结合基团，y是具有等于1―30个碳―碳单共价键的链长的间隔基或者不存在，R4是H或者卤素或者CH2O－R3；R1、R2和R3是相同的或者不同的并且是氢、甲基、乙基、烷基、链烯基、羟基化烷基、羟基化链烯基或者包含烷基、链烯基、羟基化烷基或羟基化链烯基的醚，任选地是来源于具有等于3―24个碳原子的碳链长的饱和或不饱和脂肪酸的酰基，其条件是R1、R2或者R3的至少一个包括具有3―24个碳原子的饱和或不饱和碳链的基团，或者使细胞暴露于具有式的化合物，在式中：w是核酸，x是非氨基酸或者非肽核酸结合基团，y是具有等于1―30个碳―碳单共价键的链长的间隔基或者不存在，R5是烷基、链烯基、羟基化烷基、羟基化链烯基或者包含烷基、链烯基、羟基化烷基或羟基化链烯基的醚，任选地是具有等于3―24个碳原子的饱和或不饱和碳链的链长的酰基，其条件是R5包括具有3―24个碳原子的饱和或不饱和碳链的基团。

The invention also encompasses nucleic acid molecules encoding the albumin fusion proteins of the invention, as well as vectors containing these nucleic acids, host cells transformed with these nucleic acids and vectors, and methods of making the albumin fusion proteins of the invention using these nucleic acids, vectors, and/or host cells.

本发明还包括编码本发明的白蛋白融合蛋白的核酸分子，以及含有这些核酸的载体，由这些核酸和载体转化的宿主细胞，和使用这些核酸，载体和/或宿主细胞制备本发明的白蛋白融合蛋白的方法。

The invention also encompasses nucleic acid molecules encoding the albumin fusion proteins of the invention, as well as vectors containing these nucleic acuds, host cells transformed with these nucleic acids and vectors, and methods of making the albumin fusion proteins of the invention using these nucleic acids, vectors, and/or host cells.

本发明还包括编码本发明的白蛋白融合蛋白的核酸分子，以及含有这些核酸的载体，被这些核酸和载体转化的宿主细胞，以及使用这些核酸、载体和/或宿主细胞制备本发明的白蛋白融合蛋白的方法。

A method of extracting nucleic acids from blood comprises contacting blood cells, preferably after lysing with an activated solid phase at one pH to immobilise the nucleic acids and then removing the nucleic acids at a higher pH when the charge has been reversed or neutralised.

一种从血液中提取核酸的方法，该方法包括：将血细胞，最好于细胞裂解之后，在一个pH值时与活化固相接触以固定核酸，然后在电荷逆转或变为电中性时在较高pH值下取出核酸。

Complete genome sequences of 20 viruses in Bunyaviridae loaded from NCBI were analyzed by two biological softwares of DNAMAN and DNASTAR. The results indicated great variability in both nucleic acid sequence and protein structure between plant viruses and animal viruses:(1) only plant viruses could encored NSm in M genome;(2) the length of nucleic acid sequence and protein sequence was different;(3) GC content of nucleic acid of animal viruses was higher than plant viruses of that;(4) protein topology analysis by online software SMART discovered that there was significant difference for the structure of glycoproteins G(subscript nG between animal-infecting and plant-infecting viruses;(5) usually, protein of plant-infecting virus had more complicated structure, more low compositional complexity and had N-Signal peptides except INSV.

本文从NCBI数据库下载具有完整基因组序列的布尼亚科病毒的5个属20种病毒的序列，用生物学软件DNAman，DNAstar进行比对分析，发现布尼亚科病毒属中的植物病毒在核酸序列及蛋白结构上与动物病毒有很大差异：(1)只有植物病毒的M基因组能编码NSm运动蛋白；(2)植物病毒和动物病毒在核酸序列和蛋白质序列长度均有差异，表明该科病毒是进化速度较快的病毒；(3)植物病毒核酸序列的GC含量低于动物病毒；(4)通过SMART网络软件进行蛋白质拓扑结构分析发现植物病毒和动物病毒在糖蛋白GG的结构上存在显著差异；(5)植物病毒糖蛋白结构较为复杂，有较多的紊乱区域，除INSV外，其他病毒都具有N端信号肽。

Nucleic acid former cross can detect according to its content and cent is a cell inside former inside a cross and constituent section former what its use a cross; foundation bougie and place should detect of nucleic acid differ to be able to be divided again for cross of DNA-DNA, RNA-DNA, RNA-RNA.

核酸原位杂交可根据其检测物而分为细胞内原位杂交和组织切片内原位杂交；根据其所用探针及所要检测核酸的不同又可分为DNA-DNA、RNA-DNA、RNA-RNA杂交。

Antigene peptide nucleic acid specifically inhibits MYCN expression in human neuroblastoma cells leading to cell growth inhibition and apoptosis Roberto Tonelli,Stefania Purgato,Consuelo Camerin,Raffaele Fronza,Fabrizio Bologna,Simone Alboresi,Monica Franzoni,Roberto Corradini,Stefano Sforza,Andrea Faccini,Jason M. Shohet,Rosangela Marchelli,and Andrea Pession Department of Pediatrics, University of Bologna,Sant'Orsola-Malpighi Hospital, Bologna, Italy; Department of Organic and Industrial Chemistry, University of Parma, Parma,Italy; and 3Center for Cell and Gene Therapy, Texas Children's Cancer Center, Baylor College of Medicine, Houston, Texas Abstract We developed an antigene peptide nucleic acid for selective inhibition of MYCN transcription in neuroblastoma cells, targeted against a unique sequence in the antisense DNA strand of exon 2 of MYCN and linked at its NH2 terminus to a nuclear localization signal peptide.

抗原肽核酸抑制MYCN具体表达的神经母细胞瘤细胞，导致细胞生长的抑制和凋亡罗伯托利，斯特凡Purgato ，苏埃洛卡默琳，拉菲尔Fronza ，梅奥尼博洛尼亚，西蒙娜Alboresi ，莫妮卡弗兰索尼，罗伯托Corradini ，斯特凡诺斯福尔扎，安德烈Faccini ，杰森先生Shohet ， Rosangela Marchelli ，和安德烈Pession儿科，博洛尼亚大学，圣' Orsola -马尔皮基医院，博洛尼亚，意大利；有机部和工业大学化学帕尔马，帕尔马，意大利和3Center的细胞和基因治疗，德州儿童癌症中心， Baylor医学院，得克萨斯州休斯敦摘要我们开发了一种抗原肽核酸的选择性抑制神经母细胞瘤MYCN转录细胞，针对一个独特的序列反义DNA链的第2外显子，并与在MYCN 其氨基端为核定位信号肽。

Objective:To observe the effectiveness of BCG polysaccharide and nucleic acid injection and podophyllum resin on condyloma acuminatum.Methods: Podophyllum resin was applied on the warts,then inject BCG polysaccharide and nucleic acid injection 1ml/qod for 18 times.

目的：观察卡介菌糖核酸注射液联合鬼臼根树脂溶液治疗尖锐湿疣疗效方法：鬼臼根树脂溶液涂于疣体处，肌注卡介菌多糖核酸注射液，每次1ml，隔日1次，18次疗程。

As to thymine, the bond energy of the ion with the most stable tautomer of the free nucleic acid base is the weakest among the three tautomer＇ s complexes, and that of the ion with least stable tautomer of the free nucleic acid base is the strongest .

在互变异构体复合物中，键能是依赖于金属离子成键的位置，而总能量取决于全部的原子和它们之间的相对位置，这可能是造成几种互变异构体复合物键能和总能量变化趋势并不一致的原因。

B. Each kind of nucleic acid is composed of three parts: Molecular azotic basic group + member pentose + molecular phosphoric acid radical, DNA is by C, H, O, N, the P five kind of elemental compositions. c. The nucleic acid azotic basic group may divide into four kinds: Guanine, thymine, adenine, cytosine d.

b。 每一种核酸由三个部分所组成：一分子含氮碱基+一分子五碳糖+一分子磷酸根，DNA都是由C、H、O、N、P五种元素组成的。

In the days of sailing vessels the crew were afraid they would be becalmed on the ocean.

在使用帆船的时代，船员们担心他们会因为无风而无法在海洋上航行。

As long as foreign donors pay the PA's salary bill, few expect a new intifada.

只要外国继续为巴权力机构的薪水买单，希望发动新暴动的人便寥寥无几。