查询词典 nuclease

A preliminary study on substrate specificity of Lumbricus Bimastus nuclease DNase E degradation studies showed that there were differences in degradation rate of the DNase E to different substrates.

双胸蚓组织核酸酶底物特异性的研究通过DNase E在最适反应条件下对几种不同底物降解速率的研究表明DNase E降解底物存在差异性。

Methods1. Preparation of rude extracts of Lumbricus Bimastus nucleases and its purification Using DNA-casting SDS-PAGE and agarose gel to monitor the activity of the nuclease in the process of extraction.

双胸蚓组织中核酸酶粗提物的制备及核酸酶的纯化以SDS-PAGE-DNA功能胶和琼脂糖凝胶电泳测活相结合作为双胸蚓组织核酸酶提取过程中核酸酶活性的监测体系。

Positioning is examined by using micrococcal nuclease to examine cleavage sites relative to a restriction site at the 5 end of the gene.

最初基因会展现一个核小体的模型，它是由穿过基因一段有效距离的启动子构成的；在3ˊ端范围中定位作用遗失。

The spheroplast suspension (200 ul) was transferred into a microcentrifuge tube, supplemented with 150–350 units/ml micrococcal nuclease (Fer- mentas), and incubated at 37°e for 5 min.

暂停的原生质（ 200 UL ）的是转入微量管，补充与150-350单位/毫升微球菌核酸酶，并培养在37 ° E的5分钟。

Core particles ) Particles resulting from micrococcal nuclease digestion of nucleosomes, consisting of 140-bp DNA and the histone octamer of a nucleosome.

在真核生物中，双螺旋的 DNA 分子围绕一蛋白质八聚体进行盘绕，从而形成特殊的串珠状结构，称为核小体。

This method conformed the following conditions: spleen phosphodiesterase and micrococcal nulcease for digesting reagent and the concentration, temperature and digesting time of the two enzymes;the components of p32-post label alleviant and labeling time;the concentration of p1 nuclease; the concentration of T4 polynucleotide kinase for catalysing and labeling reagent;the components of bidirectional spreader and the spreading time ,etc.

确定了采用脾磷酸二酯酶和微球菌核酸酶消化底物以及两种酶的使用浓度、温度及消化时间；确定了P32标记缓冲液的主要成分及标记时间；确定了P1核酸酶的浓度；确定了T4多核苷酸激酶催化标记底物的浓度；确定了双向展开剂的主要成分及展开时间等。

With the increase of concentration of aqueous extract from Peganum multisectum increased, root vigor, the contents of chlorophyll, soluble protein and nucleic acid in roots and shoots of alfalfa seedlings decreased, while the activities of protease and nuclease, the contents of O2ˉ, H2O2 and malondiadehyde increased, the activities of superoxide dismutase, catalase and peroxidase first increased and then decreased.

幼苗根系活力和叶绿素、可溶性蛋白质、核酸含量随水浸液浓度的提高而降低，蛋白酶和核糖核酸酶活性及超氧阴离子（O2ˉ）、H2O2和丙二醛含量则增加，超氧化物歧化酶、过氧化氢酶和过氧化物酶活性呈先升后降变化。

Human pericardia from cadaeric donors were treated sequentially with hypotonic buffer, SDS in hypotonic buffer, and a nuclease solution.

将来自尸体供体的人类心包先后用低张缓冲液、加有SDS的低张缓冲液和核酸溶液处理。

Thus it is presented an effective RNA extraction method based on the nuclease adsorbent macaloid, poly vinyl pyrrolidone, and high concentration of KAc and ethylene glycol monobutyl ether, which has successfully extracted high-quality RNA from many materials difficult to RNA isolation, such as RNase-rich rabbit liver, plant and microbial tissues rich in polysaccharides, lipids and polyphenol compounds.

现利用硅藻土对RNase的吸附性，结合PVP、高盐及乙二醇丁醚沉淀等处理，建立了一种广泛适用的RNA提取方法。在富含多糖的玉米胚乳，富含RNase的动物肝脏，多酚多油脂的银杏、麻疯树以及木霉、酵母等10多种RNA提取困难的动、植物与微生物材料中都提取出完整性好，得率高的RNA。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。