查询词典 nuclear

Abstract］ AIM:To investigate the role of nuclear factor κB in the induction of iNOS gene by TNFα and LPS in endothelial cells and the effect of antioxidant on the induction of iNOS. METHODS:Nitrite was determined based on Griess reaction. iNOS mRNA was analyzed using Northern blot. NFκB in the cell nucleole was detected with electrophoretic mobility shift assay.RESULTS:(1)NO production and iNOS mRNA expression induced by LPS and TNFα was blocked by pyrrolidine dithiocarbamate or N-acetylcysteine.

研究表明内皮细胞激活时iNOS表达的调控主要发生在转录水平。iNOS基因的启动子区域含有多个转录因子的结合位点包括NFκB、AP-1、NF-IL6，Oct-1等［2］，而核因子κB(nuclear factor κB， NFκB)被认为在多种参与炎症反应的蛋白质因子的基因调控中担任重要角色，因而可能是内皮细胞激活的调控中一个重要转录激活因子［2，3］。

Nuclear Run-on Assay was used to address the contribution of transcriptioninitiation to the regulation of five histones by YSV. Actinomycin D inhibitor studies were used to detect the mRNA half-life of five histones. SLBP weremeasured by Western blot assay to identify regulation of YSV on Pre-mRNAsplicing.

应用Nuclear Run-on Assay观察YSV对人肝癌BEL-7402细胞的五种组蛋白转录效率的影响；应用放线菌素D转录抑制法观察YSV对组蛋白mRNA半衰期的影响；应用Western blot检测SLBP的表达水平观察YSV对组蛋白mRNA加工的影响。

The main study of this dissertation is shown as follows.1.Two new amphiphilic steroid derivatives(N-cholesteryl succinyl glucosamine, CSG and N-deoxycholyl glucosamine,DCG) were designed and synthesized.Their chemical structures have been confirmed by means of Fourier Transform Infrared Spectrometry,Nuclear Magnetic Resonance Spectrometry and Mass Spectrometry.

设计、合成了新型的两亲性甾族衍生物—N-胆固醇琥珀酰基氨基葡萄糖(N-cholesteryl succinyl glucosamine，CSG)和N-脱氧胆酰基氨基葡萄糖(N-deoxycholyl glucosamine，DCG)，并采用傅立叶红外波谱仪(Fourier TransformInfrared Spectrometry，FT-IR)、核磁共振波谱仪(Nuclear Magnetic ResonanceSpectrometry，NMR)和质谱等分析仪器对原料、中间产物和最终产物的化学结构进行表征。

NF-κB is a kind of important nuclear factors which are relative to lots of cellular activities such as activation of immunocyte, development of T and B lymphocyte, stress reaction, cell apoptosis and so on. NF-κB exists in almost all types of cytoplasm in the unreactive form of heterodimer or homodimer.

核转录因子κB(nuclear transcription factorκB， NF-κB)是一类关键性的核转录因子，通常以同源或异源二聚体非活性形式存在于几乎所有类型细胞的胞质，其与免疫细胞的活化，T，B淋巴细胞的发育，应激性反应，细胞凋亡等多种细胞活动有关。

The amniotic fluid and allantoic fluid of chicken embryo have been analyzed and the resonances of most substances in them were assigned by 1-dimention and 2-dimention Nuclear Magnetic Resonance methods. This work could be the basis of quantitative analysis of metabolites and studying the metabolites changing between the amniotic and allantoic fluid during the hatching process. Also, it provided a new way to study the process of embryo developing.

用一维及二维液体高分辨核磁共振(Nuclear Magnetic Resonance，NMR)方法综合分析了鸡胚羊水和尿囊液的成分，对其中的大多数谱峰进行了归属，发现了一些未经报道的小分子代谢物，为研究胚胎发育过程中各个胚囊中体液的交换和小分子的代谢以及进一步对发育过程中代谢物含量变化的定量研究打下了基础，并为研究胚胎发育的过程提供了新的思路。

In this paper, Attacus ricini nuclear polyhedrosis virus was studied. The molecular weight and pi of polyhedrin and structural polypeptides were determined by vertical slab SDS-PAGE and thin-layer isoelectrofocusing electrophoresis. The restriction pattern of ArNPV DNA was assayed by 5 restriction endonucleases.

本文较为系统的研究了蓖麻蚕核型多角体病毒(Attacus ricini nuclear polyhedrosis virus，简称ArNPV)形态特征和生化特性，并预测了多角体蛋白的二级结构，为开发利用蓖麻蚕核型多角体病毒这一生物资源提供了理论参考。

OBJECTIVE:To study the effect of Defibrase on fibronectin and proliferating ceil nuclear antigen in experimental traumatic proliferative vitreoretinopathy.

目的探讨蛇毒降纤酶对兔眼外伤性增生性玻璃体视网膜病变(proliferative vitreoretinopathy，PVR)纤维连接蛋白(fibronectin，FN)、增殖细胞核抗原(proliferating ceil nuclear antigen，PCNA)的影响。

In this study, Mono-nuclear cells containing hematopoietic stem/progenitor cells were isolated from umbilical cord blood of normal full-term deliveries, healthy adult and heterocellular HPFH patient bone marrow by density gradient centrifugation. After simple purification and expansion in vitro, MNCs were induced to erythroid cell differentiation using different culture systems and cytokines including Epo, IL-3 and GM-CSF.

本研究首先利用密度梯度离心方法，从正常成人和异细胞型遗传性胎儿血红蛋白持续存在综合症家系患者的骨髓组织、健康足月正常分娩胎儿脐带血中分离得到了含有造血干／祖细胞的单个核细胞(Mono-Nuclear Cell，MNC)，进行初步纯化及体外扩增后，组合使用Epo、IL-3、GM-CSF等细胞因子，将造血干／祖细胞向红系方向进行诱导培养。

However, the recent discoveries are overturning the traditional opinions. Nuclear-born tRNA can be exported out of nucleus, while cytoplasmic-residing tRNA can also be retrogradely transport into nucleus.

然而，最近的研究成果在颠覆传统观念，tRNA不但能被转运出核，还能被逆向转运入核。2008年，新概念&tRNA核质动态分布&(tRNA nuclear-cytoplasmic dynamics)被提出，取代tRNA核质运输，描述tRNA在细胞核、质间的流动。

Photomicrographs of retrogradely labeled neurons in the trigeminal ganglion after Fast blueand Nuclear yellow injecting into the caudal subnucleus of spinal trigeminal nucleusand the nucleus of the solitary tract,respectively.

图A、B分别注射Fast blue和Nuclear yellow入三叉神经乙肝脊束核尾侧亚核和孤束核后三叉神经乙肝节内的逆行标记孩子细胞。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。