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Hairy roots were induced from tobacco leaves with Agrobacterium rhizogenes LBA1334 harboring agropin type PRil855 and binary vector PIG121HM carrying a kanamycin resistant gene nptll and GUS gene and the good hairy root clones were selected. The root with many root hairs and branches grew vigorously toward all the direction on MS medium without plant hormone and on kanamyain containing medium (km:30mg/L). The structure of the hairy root tip is different from that of ordinary root. Witch was not typical root cap maybe the main cause of losing the geotropism of hairy root. Plantlet regenerated from hairy root when cultured on Ms medium without plant hormone. The nicotine content of the hairy root was little higher than that in the natural roots.

本研究通过发根农杆菌工程菌LBA1334(含有野生型pRi1855质粒和pIG121HM双元载体质粒，编码NPTⅡ基因和GUS基因)转化烟草沙姆逊，获得了具有卡那霉素抗性烟草发状根，并筛选出良好的单克隆株系，该发状根具有典型的发状根特性(多根毛、多分支、在无激素培养基上快速生长)，通过根尖压片分析，烟草发状根不具备典型的根冠；发状根中烟碱含量1.61％稍高于与天然栽培品种母体根的含量(1.5％左右)，发状根向培养液中释放烟碱，释放量最多时占总烟碱量的81.1％；在无激素的培养基上获得了再生植株。

Applying this mental experience to myself , I feel a different me that was so different from the before one . That feeling was so real and tangible not illusionary that I believe I found the right answer this time .

找到了问题的根源，经过一定的心理体验，立刻觉得自己和以前的自己像换了一个人似的，这种改变是实实在在的而非虚无飘渺的东西，可以清楚的感觉到。

But I conceived of it not because of the literalness of my mind, or because our present circumstances are quite different from those of the past (nothing can be more natural than that, and one is not obliged to remain a writer forever: not any more so than to stay a prisoner).

但我构思这封信并不是因为我头脑呆板，也不是因为我们目前的环境跟从前很不一样（没有比这更自然的了，并且一个人不必非得继续当作家不可：就像不必非得继续做囚徒）。

The positive rate in colonic cancers of different grade was not significafltly different from that in their near-by tissues.

分化低的大肠腺癌癌旁组织中的细胞凋亡活动较活跃，有利于肿瘤的浸润与破坏。

But there is defect in present commonly used calculation formula of rockfall movement characteristics that there is no parameter can characterized the mass of the rock. So the computed result can not reflect the influence of the different rock mass to the movement characteristics and is quite different from the actual movement of falling rock.

但目前普遍采用的落石运动特征计算公式中存在没有表征落石质量的参数的缺陷，这使得计算结果不能体现落石质量的差异对其运动特征的影响，且与落石实际运动过程存在较大差异。

Fourth, the screen soft not to hurt the eyes: different from CRT technology, LCD screen does not flicker, can reduce the display of eye injuries, eye fatigue is not easy.

四、画面柔和不伤眼：不同于CRT技术，液晶显示器画面不会闪烁，可以减少显示器对眼睛的伤害，眼睛不容易疲劳。

There are the following several points different from existing finite element eigenanalysis methods for asymptotic fields near the crack tip:(1)The jump-off that educes the formula is different.(2)The form of the element is a ad hoc element.(3)The singular transformation technique is not used in the assumption of displacement fields surrounding the wedge tip.

该方法与过去原有求解裂纹端部近似场的有限元特征分析方法有几点不同：(1)导出公式的出发点不同；(2)单元形式为高次内插单元；(3)尖劈端部邻域内的位移场假定没有采用奇异变换技术。

Quite different from fox's slyness or other animals' smartness, the specific characteristic reflected on tamed animal can be called human intelligence, which is not from inheritance or reasoning, but is from sympathy—a feeling mixed with love and attachment.

但驯化动物身上的这种可称为人类才智的东西，非常不同于狐狸的狡猾或动物的聪明。驯化动物的通情达理不是来自推理，也不是天生的，而是来自同情，来自一种爱和依恋的感觉。

Lavigne describes the track as "different…but not different to stray away from who I am and what I am."

avril在描述这首歌时说"它是特别的，但又不是偏离那个真正的我的。"

Bow characteristic: Knew very well the open country seeks livehood the skill, excels to hunt for the wild animal; Is very high in the carte and tierce heaven share, but does not understand the true profound carte and tierce; Does not select food, most likes the fragrant barbecue, vainly hoped for eats each kind of different game; To mountain under world all and sentiment of the male and female ignorant chong ignorant, after when descends a mountain, often by own idea weight judgment, causes trouble and makes an ass of oneself all many; Thought the myriad things equality, the birth and death, the law of the jungle all are in society the habit, cannot have the idea which the monster and the human must oppose, the innate its package cares about; Meets matter of the trouble to be able to trade a direction to ponder that, does not like from seeking the worry, but regarding the matter which already recognized, processes very the straight line thought.

对山下世界一切和男女之情懵憧无知，等到下山之后，常以自己的想法衡量判断，闯祸和闹笑话都不少；认为万物平等，生老病死、弱肉强食皆是世间常态，更不会有妖和人必须对立的想法，自有其包容心；遇麻烦之事会换个方向思考，不喜欢自寻烦恼，不过对于已经认定的事，处理起来很直线思维。一个从小在山上长大的野人走出大山，软星设定的这个男主似乎并不稀奇，纯如白纸的男主多年来用的也不少了，但是软星为野人设定的对白和桥段让这个野人变得不凡，有的时候看到他的对白都不禁感叹编剧太有才了……初期和韩菱纱大量的搞笑对白自不用说，后期和慕容紫英谈到望舒剑的用途那段也让人笑喷。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。