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Results: ApoD was observed positively expressed in type2 astrocytes and oligodendrocytes from O2A lineage, while it was not expressed in type1 astrocytes from T1A lineage. Accordingly, we can verify the phenomenon found in the study of T1A and T2A gene different expression profile that ApoD mRNA was highly expressed in T2A and lowly expressed in T1A. Conclusion: It can be concluded that lipid metabolism related genes such as ApoD play important roles in the generation and differentiation of O2A lineage, which closely correlate with internal mechanism of lipid metabolism in the brain and myelogenesis.［Key words］astrocyte(T1A, T2A); oligodendrocyte; O2A lineage; apolipoproteinApoD

我们已采用基因芯片技术在mRNA水平研究两型星形胶质细胞的基因表达谱差异，发现ApoD在2型星形胶质细胞中差异高表达，故本文拟用激光共聚焦双重免疫荧光标记技术进一步在蛋白质水平观察ApoD在体外分化成熟的两型星形胶质细胞以及少突胶质细胞中的表达情况，以期为我们深入研究ApoD等脂代谢相关基因在脑内脂质代谢和神经髓鞘发育机制中所起的作用提供有用的实验依据，依此探讨胶质细胞发生和分化的信号机制。

This paper therefore examines the development of "the folklore Pai-Chang of Peking opera aria in Tainan city" through literature review depth-interview oral history and participant observation The concept frame is culture organism as the vertical axle and elements of a folklore activity as the transverse axle There are several significant findings about the general picture of this subject: During the late period of Japanese colonial era Tainan local people who played Peguan music learned Peking opera aria and tried to bring Peking opera aria into the folklore Pai-Chang which was one kind performance and traditional custom of Peguan music Some people who came from other places and played Peking opera aria had also known or learned "Pai-Chang" with touching Tainan local amateurs "Pai-Chang" used to be performed by amateurs for worship of god or goddess or for seasoning and wedding celebration without rewards Amateurs volunteered to perform "Pai-Chang" only for their duty temples and fraternity of Peking opera aria The majority of the audience is people who perform or like "Pai-Chang" or who live nearby There is a fixed pattern of the performance including the repertoire and the arrangement of the band The main meaning of this performace is to pray God's blessings therefore the procedure of "Ban-Hsian" is the most essential and ahead of Peking opera aria singing With time goes by the number of the amateurs has dropped; yet the demand for the worship ceremony remains; hence some of the amateurs have been trained to become professional performers Besides although the idea and the meaning of "Pai-Chang" remains the pattern of the performance has become simplified and rigid which is because of the performers' number decreasing and market adapting Nowadays the whole ecosystem of the folklore Pai-Chang of Peking opera aria in Tainan city keep changing which is mainly influenced by the transition of the society(such as the time and space of the industrial and commercial society being compressed and most people's pursuing of specialization and utilitarianism etc ) According to the findings in this paper the phenomenon can be regarded as the subject matter both of Peking opera and folklore studies For the studies of the Taiwanese culture however the subject not only enriches the subject matters of the Taiwanese culture(signifies the localization of Peking opera and a new pattern of Taiwanese folklore) but also reflects the characters of diversification integration adaptation and compromise of Taiwanese culture which used to be influenced by different regimes and the historical contexts Facing such a multicultural context this paper thus suggests researchers should show an open-minded attitude toward the complex of the Taiwanese culture studies and then important phenomenon would not be ignored or misunderstood

代表了什麼意义？对於上述疑惑，查既有研究成果，不消没有论述者，具体提即「京调排场」现象的可说没有，基於此，有必要对台南市及其周边之民俗性京调排场，不管是语汇来由或者其现象本身，做一番研究。本研究透过文献、访谈、口述史、参与观察等方法，以文化有机体之纵的发展概念为经，以民俗表演之横切面各主客要素为纬，试图先对此文化现象调查并推论出一概貌：台南市民俗性京调排场的形成，是在京调传入台南，台南本地北管子弟改习或兼习京调后，结合北管音乐文化所固有的排场表演型态及民俗演出传统，融合而成，形成时间至迟在日治晚期。而一些不同时期的外来京调人士(如原本生长於其他地区、后移住台湾的票友，和中国大陆琴师等等)，也曾透过与当地子弟的接触，而习得或知晓「排场」语汇以及本地京调排场习俗者。此项音乐民俗表演在过往多由业余子弟於神诞或民众婚俗做寿时，对其有义务或交陪的庙宇及子弟间做酬酢性的演出，不收报酬；聆赏者多是京调同好与邻近民众，也有少数路过者；演出模式包括节目安排、乐队编制皆有一定；表演意涵上以「扮仙」祈求神明赐福人间最为重要，因此排仙必不可少，且一定先於京调演唱。随著时间推移，京调排场表演活动的各要素都有变动，惟变迁速度不一，大体而言，业余表演者锐减，人们对酬神娱神节目的需求仍在，因此一些过往的业余表演者遂逐渐转型为职业表演者。此外，虽然表演意涵不变，但在职业表演人员少、又要适应市场的情况下，表演模式亦逐渐流於简化、僵化。目前京调排场整体生态仍在转变的过程中，而这种变化应与工商社会时间空间的被压缩、事事追求专业化与尼Q化等因素有关。再者，据此概貌，在结论部分厘清其意义、定位以及其对台湾文化研究之启示。台南市及其周边之民俗性京调排场现象，可视为京剧研究与民俗研究的一环，然而对於台湾文化研究，除了其题材本身标志了京剧的本土化与台湾民俗活动之新类型，进一步扩充整体台湾文化研究的内容外，更重要的是现象背后所反映的台湾文化的多元融合与妥协适应取向。台湾的海岛地理位置与长期被不同文化背景政权所殖民的历史，其文化自然容易随社会环境改变而弹性适应变迁，呈现多元纷呈、多重覆叠的特色，因此展望未来，从事台湾文化研究应秉除单一观点，保持一定程度之开放与客观胸襟，方不忽视或误识更多重要的文化现象。

Hangzhou Tianma Textile Machinery Co., Ltd. is the earliest in the non-woven fabrics for professional research and Production business, products related to the scope of the various professional rapier spinning Poncirus the field of production, from the beginning of the GA731 radius of the rapier-based development to the present HGA732 series looms, not only to meet High-end textile fabric weaving, and particularly good at some special professional-woven fabric, such as: a common standard loom, the production of silk yarn and high-precision electronic fabric of electronic coiling off high-end electronic Dobby looms, production All kinds of terry cloth towel loom, wide loom (Reed said the biggest increase was 4.6 meters), all kinds of industries with a cloth loom, Shaluo fabric looms, the Zhuang Shibu electronic Jacquard loom, the production of different warp The double-shaft looms, but also provide root Zelkowa acuminata special requirements of large package of cloth, Vol device as a whole and not for the cheese-and absorbed by the device.

杭州天马纺织机械有限公司是国内最早从事无梭织机专业研究与生产的企业，产品范围涉及到剑杆织机的各种专业纺枳品生产领域，从最开始的GA731桡性剑杆织机为基础发展到现在的HGA732系列织机，不但能满足高档纺织面料织造，并且特别擅长于一些特殊专业织物的制织，如：通用的标准型织机、生产真丝与高支纱等精细织物的电子卷取电子送经电子多臂高档织机、生产各种毛圈织物的毛巾织机、宽幅织机（最大公称筘幅为4.6米）、各种产业用布织机、纱罗织物织机、电子大提花的装饰布织机、生产不同经纱的双层经轴织机等，还可根椐特殊要求提供大卷装的机外卷布装置和用于不整经的筒子经架与匀经装置。

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

He does not say what he thinks being transcendental means, and he says he is not going to consider 'whether, in general, such a form of argument is permissible or fruitful'.6 He says the argument is also transcendental in another (presumably non-Kantian) sense: in proving the existence of a class of entities different in kind from 'sensa'.

他没有说他所认为的&先验&的意思，但他说他不会考虑&一般来说，这类论证是否是可允许的和富有成效的&这类问题，他说这类论证有另一类非康德的意义：提供对一类实体的存在的证明。

He does not say what he thinks being transcendental means, and he says he is not going to consider 'whether, in general, such a form of argument is permissible or fruitful'.6 He says the argument is also transcendental in another (presumably non-Kantian) sense: in proving the existence of a class of entities different in kind from 'sensa'.

他并没有说他怎么看先验论证，他说他不打算考虑&总的来说这么种论证是否可行或有所收获&。他说这类论证亦是另外一种意义上（应该就是指非康德意义上）的先验：证明某类不同于&感觉&的实体的存在。

In the present paper, the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR, and Sox gene fragments of expression from the testicle, brain and spleen were cloned using RTPCR products. The results show that Sox gene has specific expression in the testicle, brain, spleen, cardiac muscle and kindney and hasn't expression in muscle, liver and ovary. The results of clone reveal that the Sox genes of expression in the testicle are TSSox1 and TSSox 4, and those are TSSox2 and TSSox4 in the brain, and that is TSSox4 in the spleen. This suggests that the Sox gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiattion of male germ cell.

本文采用RT—PCR技术，研究了中华鳖不同组织Sox基因的表达，并通过PCR直接克隆法，分析了来自睾丸、脑和脾组织中的Sox基因序列结果表明在中华鳖的成体组织中，Sox基因在脑、心肌、肾、脾和雄性的睾丸组织中均有不同程度的表达，而在肌肉、肝脏和雌性的卵巢中则无表达，显示该基因具有组织表达特异性克隆分析显示，在睾丸组织中表达的是TSSox1和TSSox4基因，而在脑组织中表达的是TSSox2和TSSox4基因，脾组织中表达的是TSSox4基因此结果表明Sox基因不仅在性别决定中起作用，还可能在神经系统、免疫系统多种组织中起重要作用

Though analyzing and studying the following conclusions are gotten:(1) The fault features can be extracted from the unstably and stably vibrant signals in different extents by the methods of STFT, WPARMS and SOCS effectively (2) The acceleration signals not only possess far higher signal-noise rate of feature information than that of the stable, but also conceal the feature information which has not in the stable signals.

通过分析与研究，得出了如下结论：(1)短时Fourier变换、小波包AR谱、二阶循环谱均能有效处理发动机稳态和非稳态振动信号。它们都能在不同程度上提取出包含在振动信号中分析对象的故障特征。

From hear she arrives know her, go to new York from Beijing, have a year of much time only really, but I see however a lot of with the different thing in fokelore, this is not comprehensive perhaps, but, have 140 old histories as, reveal the top class and fashionable magazine of times female glamour, BAZAAR once had been created a lot of the first, we are willing to reveal the Deng Wendi that we see and understands to you, an enthusiasm, spell able, red-blooded, exciting woman.

从听说她到熟悉她，从北京到纽约，的确只有一年多的时间，但我却看到很多与传说中不同的东西，也许这不全面，但是，做为一本拥有140多年历史，展示时代女性魅力的顶级时尚杂志，BAZAAR曾经创造过很多第一，我们愿意向你展示我们看到和了解的邓文迪，一个热情、干练、布满活力、令人兴奋的女人。

Doped with different formula is the most desirable, the children can not adapt, if children drink the good Erie on easily for another brand, my home is your baby to drink from the beginning Erie Gold, the other not to drink.

不同奶粉掺杂一起是最不可取的，宝宝适应不了，如果宝宝喝伊利的不错就别轻易换牌子，我家宝宝从开始喝就是伊利金装，其他的都不喝。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。