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Therefore, it can be concluded that 1 60kD and 40kD molecules may co ordinates with each other in the solubilization of vitelline envelope and the inner jelly layer, and each plays a different role in the different time of hatching process; 2 the digestion or autodigestion of 60kD molecules into 40kD molecules is probably a natural behavior, not just resulted from manipulations.

由此可见，60kD分子降解或自身降解为40kD分子很可能是一种自然行为，而并非由于实验操作所致。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

By comparison between SMM HXRBS observation and ground observation of Hα and CaIIK lines, especially through the combined study between SMM HXRBS observation and chromospheric flare models, the results show that: in the initial stage of impulsive phase, there is a correlative relation betweem Hα line intensity and hard X-ray emission; different spike in hard X-ray flux curve represents the burst at different location; the energy taken by non-thermal electron beam is larger than the radiative loss in chromosphere; for the flare studied here, the chromospheric evapporation is explosive; if the chromosphere is in balance between electron beam bombardment and radiative loss, the column mass density at the top of chromospheric flare model can not be taken as measure from the source of electrons and then the total material between the source of electrons and the top of chromosphere can be estimated.

通过一个具体耀斑的SMM HXRBS观测结果与同时基于地面得到的Hα和CaⅡK可见光谱观测结果对比，特别是将硬x谱观测结果与色球半经验模型联立研究，结果显示出：在脉冲相上升段，硬x发射与Hα辐射之间存在对应关系；硬x线光变曲线中不同脉冲爆发分量不太可能起源于同一位置；硬x辐射所反映的高能非热电子总携带能量大于色球总辐射损失；对所研究的耀斑而言，其色球蒸发过程是爆发性的；在详细比较了非热电子色球总沉积和色球辐射损失的基础上，指出半经验模型中色球顶部柱数密度不能看成是从非热电子源度量的，从而估计了色球顶部到非热电子源之间的总物质。

Through the reasonable color utilization, to the entire space's overall assurance, the synthesis different bedroom function and the demand characteristic, from indoor design's angle embarking, the use color principle, the analysis color to the room design's importance as well as each kind of sense organ characteristic, the homochromy department's reasonable combination does not determine the different style the bedroom atmosphere, thus achieves the ideal home bedroom environment!

通过合理的色彩运用，对整个空间的总体把握，综合不同卧室功能与需求特点，从室内设计的角度出发，利用色彩原理，分析色彩对居室设计的重要性以及各种感官特点，不同色系的合理组合来确定不同风格的卧室氛围，从而达到理想的居家卧室环境！

The HPV positivity amongst the group of women with no cervical abnormality was 43%, while that in women with cervical dysplasia and cervical cancer was 81.2% PCR products from 89 HPV positive cases were genotyped by LiPA, of which 49 cases were single HPV infected concerning 11 different HPV types and the commonest type was HPV-16, occurring in 25(51%) of all single HPV infected cases. The other 40 specimens contained multiple HPV types comprising 16 different HPV types. Although double infections(n=25) prevailed, triple(n=9),quadruple(n=3) and quintuple(n=3) infections were also found. PCR products of four HPV positive cases failed to be classified by LiPA. Sequencing analysis revealed that these 4 cases were HPV-66 which was not included in LiPA probes. Follow-up data of 12 cases confirmed the HPV genotyping results. Conclusion The LiPA for HPV genotyping is easy to perform.

结果 155例妇女子宫颈刮片中HPV DNA检出率为60%，其中在宫颈细胞学检查正常或仅有炎症病变的妇女中HPV DNA阳性率为43%，而在宫颈细胞增生不良或宫颈细胞癌的妇女其阳性率为81.2%。93例HPV阳性的标本中，89例利用LiPA成功地作出了基因分型，其中单独一型HPV感染为49例，涉及11种不同基因型HPV，最常见的型别为HPV 16，占单独感染的51%(25/49)；另40例为不同型别HPV重叠感染，涉及所有16种HPV基因型别，二重感染占主导地位(n=25)，同时也检测出其它多重感染。4例HPV阳性的标本应用LiPA未能作出基因分型，DNA序列分析显示这4例为HPV 66感染，此型别不包括在LiPA所标记的探针内。12例随诊资料显示了LiPA的物异性及高度的重复性。

The so-called miracle, the so-called continuous此恨never, my dear, is now different in ancient times, not so touching earnestness, the world is changing day by day, love can also be updated from time to time, no longer deceive ourselves what is truly can not outrightly ruled out the possibility that as long as you are willing to should not the future牵绊emotion, on the believe that have been severed, the only way to be able to forge ahead in exchange for new students.

所谓奇迹，所谓此恨绵绵无绝期，亲爱的，现在不同古时，没有那么缠绵深长，天地天天在变，爱情也可时时更新，不必再骗自己有什么是真正决绝不了的，只要你愿意，不该牵绊未来的情感，就该相信已经断绝，唯有这样，才能跨步向前，换取新生。

The fresh chicken feces were obtained from different age 18 healthy chicken, and were not detectedchlortetracycline. The mixture standard solution of CTC and OTC was added in fresh chicken feces at the concentration of 0.5, 1.0 and 4.0ug/ml respectively. The chicken feces were divided to two groups,one were put in the incubator under photophobic condition; another were under the natural illumination.Samples were collected after 0, 0.5, 1, 2, 3, 5, 7, 9, 12 and 15 days, The concentration of chicken feceswas determined by HPLC. According to the theory of degradation kinetics, we fitted the changed trendof concentrations in chicken feces under different time and studied the degradation characteristics ofchlortetracycline in chicken feces.

选取不同日龄健康鸡18只，实验期新鲜鸡粪，，经检测不含四环素类药物，然后加入金霉素标准液，使得各样品中制得的相应药品的浓度为0.5ug／mL、1.0ug／mL、4.0ug／mL，分为两个实验组，第1组放入培养箱中避光黑暗条件下；第2组置于室外自然光下，定时取样，取样时间为0、0.5、1、2、3、5、7、9、12和15d，鸡粪中药物的浓度用本实验建立的高效液相色谱法测定，根据药物降解动力学原理，对鸡粪中药物的浓度随时间的变化趋势进行曲线拟合，研究金霉素在环境鸡粪中的降解动态规律。

The 352 valid samples were analyzed by SPSS. The results showed there was no distinct corresponding relationship between the product attributes and reaction time. The different key attributes from questionnaire importance rating and the shortest reaction time standards were used to regressively analyze the results of customers' overall rating (such as overall satisfaction,objective quality, recommend intention).The results indicated that the coefficiency of regression of the special attributes chosen from reaction time to overall rating was distinct, while the coefficiency of the special attributes chosen from importance rating to overall rating was not. The main conclusions are: 1. Regarded attributes can be obtained by the reaction time of brand performance rating.

本实验的有效样本为352个，通过SPSS软件进行分析统计，数据统计结果显示，在品牌表现评价反应时与其重要性问卷评定结果的相关系数的检验中，并未发现品牌表现评价反应时与品牌属性重要性评定结果之间的显著相关；将由问卷重要性评价最高标准所得到的关键属性与通过品牌表现评价反应时最短的标准所选取的关键属性的品牌表现分别对消费者的总体评价结果（如&总体满意度&、&主观质量&、&推荐意向&）进行回归分析，发现通过品牌表现评价反应时方法选取的特殊关键属性（与问卷重要性评定法选取不一致的关键属性）的品牌表现对总体评价结果的回归系数显著，而重要性评定问卷法选取的特殊关键属性（与品牌表现评价反应时法选取的不一致的关键属性）的品牌表现对总体评价结果回归系数不显著。

I wondered desperedly how long this was going on, having the feeling it explained some other things from before, but not being able to logically update with my own thinking 42 years of my life in one sudden moment, this experience is the most totering, i was fully aware, of the fact my entire thinking failde to understand what was going on, and that is aswell in fact the most interesting part of it, but aswell the cruel one, the past is completely different than in my memories was linked in terms like cause and consequence, and suddenly all seemed different, but the thinking ability of a human is clearly too slow to update and relink those facts until aswell in the longterm memorie, that feeling is awfull and something that normally humanns can only know teoretically, but feeling it happening is amazing, but terrifying.

我很惊讶这是多么的卑鄙无耻，这持续了如此之久。从一开始就试图解释一些其他的事，但对于这个突然的时刻，这不合我一生42年的逻辑思维。这是最酷刑的体验，我充分意识到，事实是，我整个的思维在理解发生了什么时是失败的，那实际也是它最有趣的部分，但也是最残酷的一种，与我记忆中与这相关的原因和结果相比，过去是完全不同的，一切仿佛突然而不同。但一个人的思维能力显然太慢更新，并且重新联系到那些事实，知道也变成长期的记忆，那种感觉是非常可怕的，某些是普通人只能够理论上知道，但感觉这是很令人惊讶的事，也是很可怕的事。

Basically, the distribution patterns of most trace metals can be divided into iron style and manganese style, both of which were controlled not only by their different remobilization degrees, but the different input from coal mining drainage, mainly influenced by pH.

Fe型元素与Mn型元素分布差异的原因可以简约的概括为：锰的界面循环极为强烈，导致再迁移程度很高；而铁的循环相对较弱，又容易受到上层硫酸盐还原作用的抑制，因此再迁移程度不高。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。