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In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

Water erosion, one day, it will not exist any more.

流水侵蚀，总有一天，它也不会存在了。

If you will do this, I will try not to think any more about escaping.

如果你愿意做，我就不再去想逃跑的事了。

Designed to mark the company's 100th anniversary, we thought that there could not possibly be any more special editions of the Veyron on the horizon, but as this year's Concorso d'Eleganza Villa d'Este approaches new images of some very unique Veyrons have surfaced.

设计的该公司的100周年，我们认为，不可能有任何更多的特别版威龙的地平线上，但今年的Concorso德Eleganza德埃斯特别墅办法的一些新的形象非常独特Veyrons有浮出水面。

"I do not believe I want to play any more," said the youngest, a black-haired beauty, turned supercilious by fortune, as she pushed a euchre hand away from her.

"我不想打下去了。"三人中最年轻的那个黑发丽人说，她因为好运当头而显得十分傲慢，这时正把一手纸牌从面前推开。

The operation requires:(1) on the petitioning process to check whether there are any more, less, wrong please!--such as unqualified to check conformity to students'rooms and clothing in storage in;(2) in a manner worthy of proper, not exorbitance, nor can it brought down.

其操作要求：(1)储帖前要检查上工序有无多帖、少帖、错帖等不合格品，检查无误后整齐、平服地储在斗内；(2)储帖的高度要适当，不可过高，也不可衔接不上造成停机。

Now to get the doctors to figure out which one it is… Feh, it's not like I need any more doctor visits.

现在需要医生来断定一下原因。嘘……好像我并不再需要去看医生。

It's not just a fertilizer any more.

它再也不只是一种肥料了。

Because mass transfer velocity is limited, Fick Law is not suitable for rapid and instantaneous mass transfer process any more.

有限质传播速度的存在，使得斐克定律对于快速瞬态质传递过程也已经不再适用。

He said,"I am not fighting for you any more"

他说："我不再为你而战。"

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。