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Methods A nested RT-PCR was used for detecting one fragment of SARS-CoV RNA in oropharyngeal swabs from 3 SARS probable patients ,4 SARS suspect patients and other 27 patients with fever in Hangzhou, and the nested RT-PCR product from one SARS probable patient was sequenced.Meanwhile in these 3 SARS probable patients, other three RT-PCR methods, including a hemi -nested RT-PCR targeted for another fragment of SARS-CoV RNA, a real-time RT -PCR and a modified nested real-time RT-PCR, were employed to detect SARS-Co V RNA.Results Two positives were found in the 3 SARS pro bable patients, and none positive in 4 SARS suspect patients and other 27 patien ts with fever, using the nested RT-PCR.

方法对2003年杭州市3例严重急性呼吸综合征临床确诊患者、4例疑似和27名医学观察者的咽拭子标本用巢式实时RT-PCR检测SARS-CoV核酸，对阳性扩增产物进行核酸序列测定，同时对3例患者的标本应用半巢式RT-PCR检测另一位点SARS-CoV核酸片段，并应用常规实时RT-PCR技术及改良实时RT-PCR技术进行检测。

I want none of your backchat!

不要跟我顶嘴！

Whatever I'm doing is none...- Is Anson Baer "The Game"?

我做什么都不关你的事-游戏是安森安排的吗？

And that his foundations were good one may see: Romagna waited for him for more than a month; in Rome, though he was half-alive, he remained secure; and although the Baglioni, Vitelli, and Orsini came to Rome, none followed them against him; if he could not make pope whomever he wanted, at least it would not be someone he did not want.

公爵狠 劲十足，势大力强；对于怎样做会赢得人心或者怎样做会失去人心，他深谙其理；在很短的时间里，他便奠定了牢固的基业。

Pfund said Bahr joined the reserves when she was 17 to earn money for school and loved being in the military even though none of her friends were interested in joining the Army .

他母亲Pfund说Bahr在17岁的时候自己挣钱上军校而且热爱军队。尽管他身边的朋友没有一个参军感兴趣的。

I forgot to bring my pen with me and none of the ballpoint pens here works.

我忘了带钢笔了，这儿的圆珠笔也全都不好用。

"The scary thing is none of this stuff comes out until 40 years after," says Bamford.

"可怕的是，这一切都不是的东西出来，直到40年后，说：" bamford 。

I replied,'He is my cousin,' and there was none of Bani Abu Manaf in the caravan except myself.

我回答，'他是我的表哥，'有没有巴尼阿布马纳夫在车队除自己了。

At the railway station in Nashville a distant relative awaited me to apprise me of the reason for my recall: my mother had been barbarously murdered -- why and by whom none could conjecture, but the circumstances were these.

出了火车站，来接我的是我的一位远亲，他把这么急让我回来的原因告诉了我：我的母亲被人残忍得谋杀了——至于凶手是谁，杀人动机是什么则无人知晓，也无从推测。

You'd think none of them had played with a Barbie doll.

你会以为她们中没有人玩过芭比娃娃。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。