查询词典 none the less

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数，结果表明：①酶-底物二元、三元复合物的失活速度明显慢于游离酶，说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用；②在脲作用下，酶的失活和构象变化均为单指数项过程，而且酶的活力丧失和三级结构变化是同时发生的，说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程；③在盐酸胍作用下，酶的构象变化为两相过程，而失活则是单指数项过程，酶分子构象变化的快相速度常数与失活速度常数基本一致，因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

I do not wish to discuss Heliogabalus, Macrinus, or Julian, who, being thoroughly contemptible, were quickly wiped out; but I will bring this discourse to a conclusion by saying that princes in our times have this difficulty of giving inordinate satisfaction to their soldiers in a far less degree, because, notwithstanding one has to give them some indulgence, that is soon done; none of these princes have armies that are veterans in the governance and administration of provinces, as were the armies of the Roman Empire; and whereas it was then more necessary to give satisfaction to the soldiers than to the people, it is now more necessary to all princes, except the Turk and the Soldan, to satisfy the people rather the soldiers, because the people are the more powerful.

至于埃利奥加巴洛、马克里诺、尤里亚诺等人，我就不打算议论了，因为他们都是十足可鄙的，所以很快就给消灭了。但是我想就上面的论述总结一下。我认为，我们这个时代的君主们要使自己政府的军队特别满意，其困难比往时减少了，因为尽管他们必须对那些军队给以某些照顾，可是如有任何困难很快就获得解决了。我们这个时代的任何一位君主都没有一支军队象罗马帝国的军队那样，同政府和地方行政当局一道根深蒂固。如果说，在罗马帝国时代，满足军人比满足人民更有必要，那末现在所有的君主，除了土耳其皇帝和苏丹之外，满足人民倒是比满足军人更有必要，因为现在人民比军人更有力量了。

The guarded glance of half solicitude half curiosity augmented by friendliness which he gave at Stephen's at present morose expression of features did not throw a flood of light, none at all in fact on the problem as to whether he had let himself be badly bamboozled to judge by two or three lowspirited remarks he let drop or the other way about saw through the affair and for some reason or other best known to himself allowed matters to more or less.

37眼下斯蒂芬愁眉苦脸。他出于友谊，就对斯蒂芬投以关怀与好奇交加的谨慎目光。然而未能弄明问题，确实一点儿也没能弄明。从斯蒂芬所吐露的意气消沉的三言两语来看，这个青年到底是被狠狠地捉弄了一番呢，还是截然相反：尽管已经看穿事情的本质，出于只有他自己才最明白的理由，却多少加以默认。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。