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In order to verify the Fluorescent-Quantitation PCR kit specific for Borna disease virus nucleoprotein, compare molecular beacon with common probe and study its practical application, the sensitivity, distinctness, repeatability and stability were tested on BDV and non-BDV infected cells, as well as non-infected controls. Human and animal samples were also tested by the kit. The lowest quantity of virus RNA detected by the kit was 2.5×101, which corresponded to 1 virus copy.

为评价博尔纳病病毒(Borna disease virus, BDV)核蛋白荧光定量PCR试剂盒的各项指标,比较分子信标探针相对普通探针的优势,并了解其实际检测效果,本课题组使用BDV OL持续感染细胞株、非BDV病毒序列转染的OL细胞、正常的OL细胞,对BDV RT-PCR试剂盒的敏感性、特异性、重复性和稳定性进行评估,同时检测部分临床病人和动物外周血液RNA。

Only 1O-day pretreatment of germinated seeds at 5℃ or 10℃ was enough to enhance the floral bud differentiation in the spring variety'Duanye 13', whereas at least 20-day pretreatment was required for the promotion of floral bud differentiation in the winter variety'Yidianhong'. Non-vernalized'Duanyi 13'plants flowered 70 days after transplanting in a warm (above 15℃) and long photoperiod (16h) growth condition, but non-vernalized 'Yidianhong'plants did not flower 90 days after transplanting in the same growth condition as'Duanye 13'.

处理温度越低和/和处理时间越长,对花芽分化和开花的促进作用越明显;春性品种'短叶13'的萌动种子在5℃和10℃条件下处理10d就能显著促进其花芽分化,而且即使未经低温处理,并给予15℃以上的生长温度和16h的长日照条件,植株也能在播种后70d内开花;但冬性品种'一点红'则需要在5℃或10℃下处理20d以上方能显著促进花芽分化,若不给予低温处理,即使给予16h的长光照条件,在播种后90d也不能开花。

In other words, underground water is an essential water resource, accounting for 41%-83% of the total. Meanwhile, the formation of limestone soil is slower.(2) Guangxi is a typical karst region. A large difference is noticed in vegetation coverage between karst regions and non-karst regions. The average coverage of bush and forest communities is 14.81% and 12.13% in the karst regions, 1.92% and 31.3% in the non-karst regions.(3) The wide distribution of rocky desertification in Guizhou Province indicates the karst environment is strongly affected by carbonate rocks'properties.

水资源以地下水资源为主,且难开发;土壤资源零星分散,土层薄,易流失;(2)现存广西区植被覆盖特征显示,岩溶石山区森林覆盖率明显低于非岩溶区,同时也暗示着岩溶石山区植被的恢复、演化慢于非岩溶区植被;(3)贵州石漠化分布特征表明岩性对岩溶生态环境的制约;(4)脆弱的岩溶生态环境制约着生态系统的人口承载力。

Include: three non-woven mask, disposable face masks, industrial masks, non-woven strip caps, disposable caps, canvas gloves, work clothes and other products.

包括:三层无纺布口罩、一次性口罩、工业口罩、无纺布条形帽、一次性帽子、帆布手套、工作服等其他产品。

The Correlation between enzyme activities and physical properties of spruce forests soil The acid phosphatase was conducive to the increase of natural capacity, capillary capacity, saturated water capacity, non-capillary capacity, total porosity, non-capillary porosity, and the decrease of bulk density; the catalase and proteinase were conducive to the increase of capillary porosity and water porosity, respectively, these results suggested that catalyze function of soil enzyme directly acted the formation of soil physical structures.

土壤酶活性与土壤物理性质的相关性酸性磷酸酶活性的增强,有助于土壤自然含水量、毛管水含量、饱和水含量、非毛管水含量、总孔隙、非毛管孔隙的增加及容重的减小;过氧化氢酶和蛋白酶活性的增强分别有助于毛管孔隙和水占孔隙的增加。

Is a company specializing in the production of electricity of standard parts, steel standard parts, automobile standard parts, standard parts pipeline, railway standard parts, the new GB standard parts, building standard parts, stainless steel fasteners, high-precision standard parts, high precision fasteners, non-standard fasteners, non-standard fasteners, standard 4.8, 8.8 standard parts, standard parts 10.9, 12.9 standard, GB standard, the new GB standard, British standard standard parts , American standard standard parts, standard parts de superscript, subscript day standard parts, construction fasteners, building hardware, light bolt, square head bolts, hexagon bolt, hexagon bolt big, hot hit bolts, steel bolts, GB30-76 bolts, 5781 Bolt, Bolt 5782, Bolt 5783, Bolt 5784, 5785 bolts, steel structure with high strength bolts connecting deputy, steel torsional shear type high strength bolts used to connect deputy, steel structure with high strength bolts connecting deputy, high-strength steel large hexagon head bolts to connect the Deputy, high strength bolts, tower bolts, rail bolt, wheel bolts, wheel screws, carriage bolts, head bolts, stainless steel bolts, screws Terrier reduction, four wire, to the foot silk, silk double-headed, square head silk, bucket-type wire, fish wire, oval wire, full-profile wire, hook the first wire, the back wire, live wire section, expansion of silk, silk expansion implosion, implosion-type expansion of silk, expansion bolts, railway screw, nut , self-locking nut, hexagonal nut, locknut, galvanized nuts, weld screws, arc stud welding with welding cylinder head screws, weld stud, weld nails without a head, cylinder head welding nails, pin, fastener, standard parts, Yamagata card, u-type cards, special bolts, special-shaped standard parts, special fasteners professional production enterprises.

河北冀南标准件有限公司是一家专业生产电力标准件,钢结构标准件,汽车标准件,管道标准件,铁路标准件,新国标标准件,建筑标准件,不锈钢标准件,高精度标准件,高精度紧固件,非标标准件,非标紧固件,4.8级标准件,8.8级标准件,10.9级标准件,12.9级标准件,国标标准件,新国标标准件,英标标准件,美标标准件,德标标准件,日标标准件,建筑扣件,建筑五金,光螺栓,方头螺栓,六角螺栓,大六角螺栓,热打螺栓,钢结构螺栓, GB30-76螺栓,5781螺栓,5782螺栓,5783螺栓,5784螺栓,5785螺栓,钢结构用高强度螺栓连接副,钢结构用扭剪型高强度螺栓连接副,钢结构用高强度螺栓连接副,钢结构用高强度大六角头螺栓连接副,高强度螺栓,铁塔螺栓,铁道螺栓,轮毂螺栓,轮毂螺丝,马车螺栓,沉头螺栓,不锈钢螺栓,缩梗螺丝,四角丝,地脚丝,双头丝,方头丝,斗型丝,鱼尾丝,鹅蛋丝,全纹丝,钩头丝,靠背丝,活节丝,膨胀丝,内爆膨胀丝,内爆式膨胀丝,膨胀螺栓,铁道螺钉,螺母,自锁螺母,六角螺母,防松螺母,镀锌螺母,焊钉,电弧螺柱焊用圆柱头焊钉,焊接螺柱,无头焊钉,圆柱头焊钉,销轴,紧固件,标准件,山形卡,u型卡,异型螺栓,异型标准件,异型紧固件的专业生产企业。

Is a professional in the health-level non-pressure vessel, chemical pressure tank, reactor, agitator tank, hydraulic lifting platform, heat exchanger, evaporator ,Carbon quartz sand filters, bag filters, cartridge filters, nanofiltration filters and the non-standard equipment design and development, manufacturing and service businesses.

广东省江门市江海区英杰特机械设备有限公司是广州英纳集团成员企业,是一家专业从事卫生级常压容器、化工常压容器、反应罐、反应釜、液压升降平台、换热器、蒸发器、活性炭石英砂过滤器、袋式过滤器、滤芯过滤器、纳滤膜过滤器及非标机器的设计与开发、生产及服务的企业。

Companies leading products are: stereotypes non-woven, non-woven acupuncture, James oil cloth, geotextile, green cloth, roof insulation cloth, the dam with a cloth, shells sleeve cotton, of cotton, cotton-song, Waterloo cotton, cotton velvet, Products 1-4 meters width and thickness of 60 to 600 grams, length and color can be produced according to customer requirements.

公司主导产品有:定型无纺布、针刺无纺布、油沾布、土工布、绿化布、保温棚布、堤坝用布、弹袖棉、复合棉、松棉、滑棉、天鹅绒棉等,产品幅宽 1 至 4 米,厚度 60 克至 600 克,长度及颜色可根据客户要求制作。

The product the company has is as follows: Automatic Bottle Arranging Machine, Two-end Sifting Counting Machine, Photoelectricity Counting Machine, Automatic Stuffing Machine, Automatic Claw Type Cap Unscrewing Machine, Transistor Electromagnetism Induction Hole Enveloping Machine, Non-Dry Tape Marking Machine, Multi-functional Non-dry Tape Marking Machine, Combination Machine of Two-end Sifting Counting and Stuffing, Liquid Canned Machine, etc.

公司现有产品:自动理瓶机、双头筛动式数片机、动塞纸机、自动爪式旋盖机、高速搓式旋盖机,晶体管电磁感应封口机,不干胶贴标机,多功能不干胶贴标机,双头筛动数片塞纸组合机、液体灌装机等产品。

METHODS: Using tissue culture technique, the maximal non-toxic concentration of luteolin in swine testicle cell culture was measured, and the inhibitory effect of luteolin on TGEV was measured by cytophathogenic effect observation and crystal violet measurement. In addition, the degrees of inhibition of the luteolin on CPE of virus in cell culture were observed by changing sequence of adding the vires and the drag. RESULTS : The maximal non-toxic concentration of luteolin was 873.39 μmol/L.

采用细胞培养法,通过结晶紫摄入量测定结合细胞病变作用,观察木犀草素在不同的加药方式下,对感染TGEV的猪睾丸细胞的保护作用、结果:TGEV病毒先吸附ST细胞再加入药液,先与TGEV病毒孵育再接种于卯细胞,或药液先与细胞孵育再用病毒感染等不同方式下,木犀草素在0.84-54.50μmaol/L浓度范围内,均能明显地提高细胞的存活数。

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相关中文对照歌词
A Call To All Non-Believers
La Luna Che Non C'e
Dell'amore Non Si Sa
Le Parole Che Non Ti Ho Detto
Non Ti Scordar Di Me
Non Angelical
L'uomo Che Non C'è
Se Non Ci Fossi Tu
Ci Sei E Se Non Ci Sei
Non-Partial, Non Political
推荐网络例句

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?