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AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

For confirmation of this ancient legend and found out the sexual improvement compounds the testosterone and NO in serum of mice were measured,the weight of sexual organs of mices was detected and the sex behaviour of mice was observed.The testosterone and NO concentration in serum of mice as indicator for screening some improving sexual compounds from Chinese chives was builted.1、The preliminary experiment of Chinese chives confirm sexual improvement functionThe sexual improvement function experiments of Chinese chives comparing with herba epimedii,herba cynomorii and radices morindae which were acknowledged as chinese herbals having sexual improvement function were carried out.The result was showed that the Chinese chives was confirmed having sexual improvement function,though its efficacy was less than herba epimedii,herba cynomorii and radices morindae.

为了证实古代医学认为韭菜的壮阳功能，揭示韭菜起温肾壮阳作用的化学物质基础，开发其功能性物质成分，本实验首先应用韭菜酒精初提液对小鼠睾酮、NO含量、性器官、性行为等指标的测定，验证韭菜是否具有改善性功能作用，然后以小鼠睾酮、NO含量等为指标对韭菜中的壮阳物质进行逐步分离和改善性功能作用筛选，以鉴定出几种改善性功能的物质。1、韭菜改善性功能的验证将韭菜与淫羊藿、琐阳、巴戟天三种有温肾补养功能的中草药进行比较，发现韭菜改善性功能作用不如淫羊藿、琐阳、巴戟天壮阳中草药的效果明显，但是韭菜还是具有显著的改善性功能作用的(P＜0.001)。

The comparisons of NO reduction efficiencies and specific NO reduction quantities under different conditions were made. The influences of oxygen, reburn fuel fraction and coal particle size on specific NO reduction quantity were analyzed.

对不同工况下NO还原效率与比脱硝量进行对比研究，分析O2浓度、再燃燃料比与煤粉细度对比脱硝量的影响规律。

With diluted anaerobically pretreated wastewater from a pig farm and Spirodela oligorrhiza, TN, NH(superscript + subscript 4)-N and NO(superscript – subscript x)-N removal pathways in shallow-water duckweed-based wastewater treatment systems were studied in summer and winter.

以稀释的猪场厌氧污水为供试水样，少根紫萍为供试生物材料，对夏、冬2季浅水体浮萍污水净化系统TN、NH(上标+下标 4)-N和NO-N（NO(上标–下标 2)-N与NO(上标–下标 3)-N之和）的去除途径进行了试验研究。

Because p53 governs NO· production by transcriptionally transrepressing NOS2, we used a genetic strategy to determine whether NO· and p53 cooperatively regulate tumorigenesis.

由于P53在转录水平反式阻抑NOS2而控制NO的产生，我们运用基因方法来判断是否NO与P53共同调控着肿瘤发生。

Then the mixture of NO〓 and unoxidized NO as well as SO〓(1000-4000ppm) in the gaseous phase is simultaneously absorbed with aqueous ammonia to produce useful chemical products.

氧化吸收技术是燃煤烟气脱硫脱氮一体化工艺技术之一，其基本思想是利用烟气中较高浓度的O〓(4-7％)，在SO〓(1000-4000ppm)存在下，在150℃左右的烟气温度下，将NO(300-800ppm)部分催化氧化成NO〓，然后转化为有用的化工产品。

Launching an official campaign dubbed "No Leave, No Life", Martin Ferguson, the tourism minister,urged Australians to use up the average of 11 days of paid but untaken leave that each has accumulated.

在开展一场名为&没有假期，就没有生活&(No Leave， No Life)的官方运动之际，澳大利亚旅游部长马丁·弗格森督促澳大利亚人用完每个人积累的带薪假期，平均为11天。

ET and NO are the biology regulatory factors produced by vascular endothelial cell.ET is a peptide with vasoconstrictive and vasodilator effect produced by endothelial cells,and NO as a powerful endothelium-derived relaxing factor,has a strong role in dilation of blood vessels.

ET和NO是血管内皮细胞产生的生物调节因子，ET是由内皮细胞产生的一种多肽，具有缩血管和扩血管作用，而NO为一种强有力的血管内皮细胞舒张因子，具有强烈扩张血管作用。

The results indicate that ① the reactor with sulfur particle size of 2.8-5.6 mm can effectively remove nitrate from nitrified leachate up to a concentration of 400 mg/L NO - 3 N at a hydraulic retention time of 5.71h;② the minimum hydraulic retention time necessary for complete denitrification depends on sulfur particle size and influent nitrate concentration;③the maximum volume...

结果表明，当HRT为5.71h，硫磺粒径为2.8－5.6mm时，最高处理浓度（以NO－3－N计）达400mg／L。达到完全反硝化所需的最小停留时间取决于硫磺粒径及进水硝酸盐浓度。硫磺粒径为2.8－5.6mm，5.6－11.2mm，11.2－16mm时，NO－3－N最高容积负荷分别为594，278，175.4g／（m3d）。NO－3－N最大表面负荷约为0.68g／（m2d），是自养反硝化的控制因素，与硫磺粒径无关。

LPS, TNF-α and NO were overproduced in patients with liver cirrhosis. NO might play an important role in hemodynamic dysfunction.

肝硬化患者血浆LPS，TNF-α和NO生成增多，NO在门脉高压形成及血液动力学紊乱中起重要作用。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。