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The effect of hesperetin and naringenin on the release of NO in human umbilical vein endothelial cells was investigated next.

采用DAN荧光法测定培养液中亚硝酸盐的含量，间接反映内皮细胞分泌NO的水平。

Hesperetin, but not naringenin can promote the release of NO from HUVEC at relative low level of endogenous estrogen, and this effect is related with regulation of gene expression of eNOS.

其中，只有橙皮素能在雌激素水平不足的情况下促进内皮细胞分泌NO，此效应涉及eNOS的基因表达调节。

Based on the cloning of Spidroin2 cDNA of Nephila clavipes (Genbank Accession No. AF441245), we constructed a prokaryotic expression vector pET-28b-Sp by double digestion.

本研究在获得生长于中国的Nephila clavipes蜘蛛牵引丝蛋白Spidroin2 cDNA（Genbank Accession No。

Although there was no significant difference in the levels of SOD, MDA, and the parameters of the renal function between the L-NAME group and control group,those indexes in the L-NAME group tended to be aggravated. Moreover, the damage in the nephric tissue of the L-NAME group was not alleviated.

给予L-NAME组大鼠与对照组相比，肾组织NO含量明显降低（P.05）；两者SOD活性、MDA水平、肾功能指标虽无统计学差异，但有损害加重趋势，肾脏组织损伤未见减轻。

We also observed the morphological changes of the kidneys. Results: In the control group,the amount of NO and MDA increased while that of SOD decreased (P.01, P.01and P.01), and the renal function and the nephric tissue were impaired on the 21st day after bile duct ligation compared with the sham-operation group.

结果：与假手术组相比，胆道梗阻后21 d，对照组肾组织中NO及MDA含量均增高（P.01），而SOD水平下降（P.01），肾功能及组织结构受损。

It is different that different dose of NO influenced on incidence rate of neuronal apoptosis.

不同剂量的NO对神经细胞凋亡的影响不同。

Results: Compared with IR group , XSOL could significantly decrease the cerebral water content, the cerebral index, the brain vascular permeability in rats of acute incomplete brain ischemia-reperfusion (P.01,P.05). Cerebral tissue morphology showed that neuronic damage of rats subjected to acute incomplete brain ischemia-reperfusion was ameliorated following administration of XSOL (2.4, 9.6g/kg). Compared with IR group , XSOL could significantly decrease the content of MDA and NO in brain tissue(P.01,P.05)and increase SOD,GSH-px activity. The levels of cerebral Glu,Asp were cut down(P.01,P.05). Conclusion: XSOL shows protective effects on cerebral ischemia-reperfusion.

结果：与缺血再灌注组比较，XSOL大、小剂量组可以显著降低急性不完全性脑缺血再灌注模型大鼠的脑含水量(P.01，P.05)、脑指数(P.01，P.05)、脑毛细血管通透性(P.01，P.05)；光镜下脑组织形态学显示各剂量组可不同程度的减轻急性不完全性脑缺血再灌注大鼠脑组织的神经元受损形态；与缺血再灌注组比较，XSOL大、小剂量组可以显著降低缺血再灌注大鼠脑组织中的MDA的含量(P.01，P.05)，升高SOD、GSH-Px在脑组织中的活性(P.01，P.05)；XSOL大、小剂量组可显著降低脑组织中NO的含量(P.01，P.05)，降低脑组织中Glu、Asp含量(P.01，P.05)。

Peysavikan no ovey means wearing gold necklaces in front, referring to someone prestigious or as precious as gold.

peysavikan no ovey 是指胸前两边垂挂金饰的意思，比喻有声望或者视对方如黄金般的宝贝。

In the cardiovascular system, the endogenous NO, CO, and H2S form three specific and relevant pathways with their corresponding enzymes including nitric oxide syntheses, the home oxygenize and cystathionine-r-lyric enzyme, whose affect can relieve the atherosclerosis.

血管系统中内源性气体信号分子NO、CO、H2S作为一种独特的血管活性物质，分别与其相应的合成酶一氧化氮合酶、血红素加氧酶和胱硫醚－r-裂解酶形成独立而又相互关联的体内重要抗氧化系统，可以减少动脉粥样硬化的严重度。

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb，用Ang Ⅱ诱导促进其增殖，采用MTT法检测细胞增殖；羟脯氨酸试剂盒检测胶原含量；ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量；流式细胞仪检测细胞周期；硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。