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Methods Spermatocytes, early stage spermatids and the later stage spermatids were separated from the adult rat testis by the velocity gravitative sedimentation technique. Using the whole mount electron microscopy, SDS-PAGE and Southwestern blot, the morphology, components of NM-L-IF and the relationship between an A-T repetitive DNA fragment and the proteins of NM-L-IF in those cells were analyzed.

利用整装电镜技术、SDS-PAGE和Southwestern blot等技术观察与分析经单位重力沉降法分离的大鼠精母细胞、早期精子细胞和晚期精子细胞的NM-L-IF的形态结构、蛋白组成及其与A-T重复序列的相互关系。

The light intensity to elicit the male's phototactic response was not significantly different in these three time periods. In terms of action spectrum, both male and females are more sensitive to ultraviolet light than to visible light, especially the spectral region between 330 and 340 nm for the females and 370 nm for the males.

台湾铗蠓成虫对短波长之紫外光激较敏感：雌成虫之最敏感波长为330~340nm，而雄成虫为370 nm，显示雌、雄虫皆较偏好紫外光，但有同的偏好波长。

Through the spectrum shaping, the bandwidth of the regeneratively amplified pulse was increased from approximately 20 nm to approximately 35 nm, which is near the bandwidth of the seed pulse.

另外用镀反射膜的平板滤光片进行了腔外光谱整形的实验，使再生放大器输出脉冲谱宽展宽到28 nm左右。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

The results show that Fe0.13[Co20Ni80]0.87 alloy fibers are characterized with thin diameters between 0.3 and 2.0 μm, with high aspect ratio, dense and smooth surfaces. The crystalline size of the alloy fibers are mainly influenced by the thermal reduction temperature and are in the range of 20 to 50 nm with a corresponding reduction temperature range of 300 to 700 ℃. The magnetic properties of the alloy fibers are largely affected by the carbon content and crystalline sizes of the alloy fibers. With increasing thermal reduction temperature, the coercivity decreases while the saturation magnetisation value of the alloy fibers dramatically increases at lower reduction temperatures. The alloy fibers prepared at reduction temperature of 700 ℃ have a saturation magnetisation value of 120 Am2/kg and a coercivity value of 10.4 kA/m, respectively.

结果表明：Fe0.13[Co20Ni80]0.87合金纤维的直径为0.3~2.0 μm，表面光滑、长径比大；组成合金纤维的晶粒大小与还原温度密切相关，当温度为300~700 ℃时，晶粒尺寸由约20 nm增加到约50 nm；该合金纤维显铁磁性，其矫顽力主要受合金中C含量及晶粒大小的影响，随制备温度的升高而降低；饱和磁化强度则主要与合金的组分相关，随还原温度的升高和合金纯度的提高而增大；经700 ℃热还原后，合金纤维的饱和磁化强度 m s为120 Am2/kg，矫顽力 H c为10.4 kA/m。

It is found that the output mirror transmissivity of the two cavities keep a certain relation when the beam radii of pump laser, 1064 nm laser and 946 nm laser in the Nd: YAG crystal are well matched.

结果表明，当Nd：YAG晶体中抽运光，1064，946 nm激光的光束半径达到合理匹配时，两个子腔的输出镜透过率之间有确定的关系。

However , Each ferromagnetic layer (VEH=100V、120V and 150V) exhibits a columnar structure perpendicular to the film surface and the film thickness of ferromagnetic and antiferromagnetic was about 20nm and 30 nm , respectively .

然而，在VEH=100V、120V and 150V条件下的Ni80Fe20/NixFe1-xO双层薄膜的铁磁层中，一种垂直於薄膜表面的柱状结构被观察到，且铁磁层和反铁磁层的厚度分别约为20 nm和30 nm。

The results indicate that all the sub-microparticles present sphere structures with diameters between 100 nm and 800 nm.

结果表明，所制得的亚微米粒粒径在100-800 nm之间，呈现球形结构。

The presence of these resonance of phosphorus in at least two types of phosphorus environments. The spectra of nanorod contained typical bands of hexahedra Ca2+ at 200 nm. The Eg values for the HA of microcrystalline, nanorod samples were 1.60and 1.64ev, respectively. The FTIR spectra of the nanorods include two types of vibration; three PO43- stretching modes at 956.5, 1 026.1 and 1 108.7cm-1 band were attributed to the v1 (PO4), v3 (PO4), v3 (PO4) modes respectively.

结果 HA纳米粒的平均尺寸为。10～30 nm；共振分析表明，磷至少以2种类型存在；在200 nm波长处，光谱包含六面体Ca2+典型的光谱带；HA纳米粒微晶的Eg值分别为1.60和1.64 ev；傅里叶变换红外光谱中包含2种振波；在956.5、1 026.1和1 108.7 cm光带处的3种PO43-曲态分别为v1(PO4)、v3(PO4)、v3(PO4)。

According to the features of Brillouin scattering in different channel, the absorption spectra of molecule iodine absorption cell ranging from 470 nm to 550 nm are emulated in this paper.

依据不同信道中布里渊散射的特性，仿真出碘分子吸收池在470～550 nm区间的吸收光谱，在此基础上确定激光雷达系统激光中心频率的选取和鉴频器的设计方法。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。