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Methods: 1. A model of Transwell has been developed for the co-culture nearly pure microglia cells and dopaminergic neurons.

研究方法 1、用原代培养的方法建立小胶质细胞与中脑多巴胺能神经元体外培养的Transwell细胞模型。

Methods: The distribution of nitric oxide synthase-containing neurons in the gastrointestinal tract of the Microtus mandarinus was studied with NADPH-diaphorase histochemistry.

用NAD-PH-黄递酶组织化学法对NOS在棕色田鼠胃肠肌间神经丛的分布进行比较研究。

We initiated a genetic screen for Drosophila olfactory genes by means of SG18.1-Gal4/UAS system: The result validates our screen as an rapid, effective approach for recovering genes controlling glomerular map patterning; From a misexpression screen of 1,515 P{GS} lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of the Drosophila antennal lobes; These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling.

本文首次利用SG18.1-Gal4／UAS基因筛选系统对果蝇嗅觉基因进行了筛选和鉴定：结果表明，所采用的方法可以快速、有效地分离到果蝇嗅觉相关基因；从1515株果蝇P{GS}品系分离到86株突变体，其中40株有明显表型，从中鉴定了23个基因，这些基因在ORN强迫表达时可以损坏果蝇嗅叶的固有结构；在筛选中发现的一些调节嗅觉图谱发育的基因可以编码新蛋白或编码参与轴突生长和细胞骨架重塑的蛋白；为了便于研究，根据基因的功能和所编码蛋白的特点，我们对分离到的基因进行了分类，即参与轴突引导和突触产生、调节转录、调节细胞骨架和功能未确定或未知的基因；并通过对嗅觉感觉器的检测发现，除了调节转录的基因外，其他基因对触觉器的数量和分布影响不明显。

To observe the influence of tumor necrosis factora on differentiation of rat mesencephalic neural stem cells,the numbers of neurons,astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using i m munocytoche mistry technique.

为观察肿瘤坏死因子对神经干细胞分化的影响，本研究应用体外扩增的新生大鼠中脑NSCs，使用免疫组织化学技术，观察了肿瘤坏死因子一。对NSC、分化及其后代细胞的影响。

Neural stem cells are operationally defined as mitotically competent, self-renewing, and multipotent cells able to differentiate along three main cell lineages in the early neurogenesis (i.e., neurons, astrocytes, and oligodendrocytes).

神经干细胞(neural stem cells，NSCs)广泛存在于胚胎脑组织中，也存在于成年哺乳动物的神经系统。NSCs具有自我更新能力，能够定向分化为神经元、星形胶质细胞和少突胶质细胞。

Methods: the spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the sma for retrograde tracing. the bilateral superior cervical ganglia and stellate ganglia were observed to find retrogradely labeled neurons. the neurotransmitters of these sympathetic fibers around sma were ascertained by using retrograde tracing combined with immunohistochemical double labeled technique.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金，观察双侧交感颈上神经节、星状神经节内hrp或fg逆标神经元的分布；采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽y、钙基因相关肽或p物质。

Objective To study the effect of nerve growth factor on thenumberal density and morphology of positive neurons ofneuronal nitric oxide synthase in visual central systemin monocularly deprived rats in critical period, and providebasic referent evidences to preventing and treating deprivedamblyopia.

中文摘要：目的：研究和探讨在大鼠视觉发育敏感期内神经生长因子对单眼剥夺大鼠视觉中枢神经型一氧化氮合酶阳性神经元数量及形态的影响，为临床预防和治疗弱视拓展思路并提供基础研究依据。

Objective To study the effect of 9 kind of main Ginsenosides on cultured spinal moter and spinal ganglinic sensory neurons growth from embryonic rat.

摘 要 目的：探讨9种主要人参皂甙单体对体外培养鼠胚脊髓运动神经元和脊神经节感觉神经元生长的影响。

Extraterritorial neuropathic pain correlates with multisegmental elevation of spinal dynorphin in nerve-injured rats. Pain 2000;86:185-194.[16]Knox RJ, Isaac L. Effects of selective and non-selective kappa-opioid receptor agonists on cutaneous C-fiber-evoked responses of rat dorsal horn neurons.

在慢性炎症及神经损伤或过敏等引起的伤害性感受中，大量的Dyn A生成，通过NMDA 受体直接或间接地产生一些炎性介质或细胞因子，从而加强神经病理性疼痛的形成，其作用机制有待于我们进一步去探讨。

With the method of multitube electrode recording, the transmitter characteristics of LA inhibiting the acoustic response of neurons in A-Ⅰ has been studied.

七运用多管微电极记录方法结合微电泳技术，探讨了LA抑制皮层AⅠ神经元声反应的递质特性。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了