查询词典 nested

Methods The provirus DNA was extracted from peripheral blood mononuclear cell in two HIV-1 antibody positive individuals initially defected. Env genes were amplified by nested-PCR, the sequence analysis of C2 - V3 of Env genes was carried out.

从2例感染者外周静脉血淋巴细胞中提取前病毒DNA，使用套式PCR方法，扩增HIV的Env基因，对其Env C2-V3及邻近序列进行分析。

Provirus DNA was extracted, HIV-1 gag genes were amplified by nested-PCR and sequenced directly with pairs of specifically designed primers, sequences were then analyzed by GCG software system to determine genotypes, and phylogenetic trees were established.

采集17名感染HIV的农村外来妇女及这些外来妇女的4例阳性配偶的外周血，提取前病毒DNA，应用套式PCR进行艾滋病病毒gag基因检测，使用GCG软件包对扩增序列进行分析，并对这些HIV感染者进行相关流行病学调查。

HIV proviral DNA present in the peripheral blood mononuclear cells from the patient and his wife was amplified by a nested polymerase chain reaction and sequenced directly.

艾滋病毒基因存在于外周血单个核细胞，从病人和他的妻子被扩增一聚合酶链反应和直接测序。

HIV proviral DNA present in the peripheral blood mononuclear cells from the patient and his wife was amplified by a nested polymerase chain reaction and sequenced directly.

艾滋病毒基因存在于外周血单个核细胞，从病人和他的妻子被扩增一聚合酶链反响和直接测序。

A method for detection of proviral human immunodeficiency virus type 1 DNA fragment encoded pol gene in dried blood spots on filter paper by nested primers polymerase chain reaction has been developed.

目的建立套式引物聚合酶链反应，用于检测滤纸干血斑中的人免疫缺陷病毒1型（HIV1）前病毒pol基因DNA片段。

Nested PCR analysis and southern hybridization analysis showed that no polymorphism between H.villosa, Pm97034 and susceptible parent Wan7 107 was detected with the clones from 6VS arm, whereas three clones from H.villosa genome DNA: RH42, RH55, and RH66 showed polymorphism. RGA6 cloned from H.villosa genome DNA was characterized identity to NBS, and show high homology to resistance genes as RPM1 and RPP13 in Arabiadopsis, LRR19 in wheat, and I2C-1 in tomato.cDNA library constructed from T.aestivum-H.villosa translocation line Pm97034 was screened by hybridization with RGA6. Four positive cDNA clones were obtained. R3-2-2 showing 60-90% identity with eukaryotic sulfite oxidases contained an open reading frame of 647bp encoding 140 amino acid, and contained a conserved Moco-dimer domain in the ORF. R6-2-2 showed an ORF containing an Euk-porin domain of 279aa with 20-40% identity to the eukaryotic voltage-dependent anion channel proteins. R8-1-1 showed a complete ORF of 1810bp encoding 493aa with 80-90% identity to plant catalases. R9-1-1 showed an ORF containing a BTB/POZ conserved domain of 204aa with 30-70% identity to pox virus and zinc finger proteins. R3-2-2 and R9-1-1 were the first cDNA clones containingconserved domain of SO and POZ respectively isolated from wheat. R8-1-1 contained a complete ORF with 81% identity to Cat-3. Aspects of the role of R8-1-1 may be same with Cat-3, and it would offer the opportunity for improvement of stress tolerance of wheat.

以RGA6为探针，筛选用抗白粉病小麦—簇毛麦易位系Pm97034构建的cDNA文库，得到了4个阳性克隆。R3-2-2与动植物的亚硫酸氧化酶(sulfite oxidase，SO)有60～90％的同源性，长度为647bp，编码140个氨基酸，具有开放阅读框并含有保守域Moco-dimer.R6-2-2与真核生物的VDAC(voltage-dependent anion chennel)蛋白有20～40％的同源性，长度为1047bp，编码279aa，是一个不完整的开放阅读框，预测结构具有Euk-porin结构域。R8-1-1与植物中已经克隆的过氧化氢酶有80～90％的同源性，长度为1810bp，编码493aa，具有完整的开放阅读框和过氧化氢氧化酶保守域。R9-1-1与动植物的POZ(pox virus and zinc finger protein)蛋白有30～70％的同源性，长度为1446bp，具有开放阅读框和BTB／POZ保守域。R3-2-2与R9-1-1是首次从小麦中克隆到的具有SO和POZ保守域的cDNA序列。R8-1-1具有完整的开放阅读框，与玉米Cat-3基因具有81％同源性，预测R8-1-1可能具有与Cat-3类似的功能，可为转基因小麦抗逆育种提供新的基因。

Nested ANOVA test showed the variance of GCPP mainly occurred among sites rather than within periphyton. Diatoms increased with the GCPP. There is a significant positive relation between the dissolved inorganic nitrogen in the water column and GCPP of periphyton.

GCPP的时空变化与底栖微藻在附生藻类生物量中所占比例和水体中的无机氮浓度有非常显著的正相关性，初步推测大鹏湾的GCPP可能是受到水体中氮的限制而有GCPP之时空变化。

Pre-eclampsia, soluble fms-like tyrosine kinase 1, and the risk of reduced thyroid function: nested case-control and population based study

先兆子痫，可溶性fms样酪氨酸激酶-1和甲状腺功能降低风险：人口为基础的巢式病例对照研究

Without handling the nested window case, both of these systems provide the desired functionality with a simple implementation.

除了是否需要处理嵌套窗口之外，这两个系统都以一个简单的实现提供了预期的功能。

Stink job always comes with a stupid boss"," snake and rat nested together. People will misunderstand the other members and you are the same.

因为中国人总是说&什麼人玩什麼鸟&、&臭味相投&、&蛇鼠一窝&，别人也会因此误会您俱乐部的成员都是像她一样，或者您本身也是这样。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。