查询词典 nested

Objectives HIV 1 C2 V3 region of env genes from sero positive people in Guangxi were amplified by nested PCR.

近年来，在广西一些地区的不同人群中出现了人类免疫缺陷病毒（HIV 1）的流行。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

The examples in the downloadable project that accompany this article show how the control can be used to implement a fully working TreeView control and a nested sortable list.

在可下载的项目，伴随着这篇文章显示如何控制可用于实施一项全面的工作TreeView控件和一个嵌套排序名单上的例子。

Inner subrule groups can be nested by enclosing each sub-group inside another set of parentheses.

内部的子规则组可以使用另外一对括号来封装以实现嵌套。

Promoted by cultural producers and marketed by advertisers, these transborder visual images are nested around various forms of urban life-style, which play an important part in socio-cultural formation and segmentation in contemporary urban China.

以香港与中国这个跨境视觉转移为例，香港最先扮演咨讯补偿或现代想象投射的角色，但当全球文化进一步互相借用，就不但是影响的跨境复制加工，而是在视觉生产方面互相协作。

A new trick using Trichogramma brassicae asvectors to transmit Dendrolimus punctatus cypovirus was developed (called"Biological missiles Dp-Ⅰ") to control the Dendrolimus punctatus in the field. Sincethen, we collect the single caterpillar from both areas used Dp-Ⅰand controlled areasevery year, and A nested-PCR-based assay for the detection of those samples wasdeveloped, The results showed that the positive reaction rate of the first year is 40～70％; and second year is 30～80％, on the other hand, the positive reaction rate of thecontrolled areas are 10～40％and 10～20％.

第二章对马尾松毛虫质型多角体病毒的流行病做了初步的调查，应用巢式RT-PCR方法检测从野外释放&生物导弹Dp-Ⅰ&的林区采集马尾松毛虫单虫样本，结果发现：2006年释放&生物导弹Dp-Ⅰ&的王福店林区的松毛虫体内DpCPV阳性率为40～70％，平均阳性率为58％，相应对照区的阳性率为10～40％，平均阳性率为24％；2005年释放&生物导弹Dp-Ⅰ&的丁家畈林区松毛虫体内DpCPV阳性率为30～80％，平均值也为58％，相应对照区的阳性率为10～20％，平均值为16％。

Result The nested PCR assay was specific and there is no cross-reaction with other parasites, such as Eimeria spp., Cryptosporidium spp., Giardia sp., Toxoplasma sp., Trichuris sp. and cattle ciliate. The assay was able to detect as low as 2.85 × 10^(-2) fg of control positive DNA. The results of detection of clinical samples revealed that the assay was coinciding with microscopic examination.

该套式PCR能特异性地扩增出牛源环孢子虫基因组DNA中相应片段，与艾美耳球虫、隐孢子虫、贾第虫等10种相关原虫基因组DNA无交叉反应；最低能检测到2.85×10^(-2)fg的阳性参照DNA；对临床样品检测结果表明PCR技术检查阳性者显微镜检查都为阳性。

The torrential rain is simulated in this paper by using a nonhydrostatic mesoscale model ARPS(V5.2, and by employing the triply single-way nested-grids.

本文所用的模式是 Oklahoma 大学风暴分析和预测中心研制的一个三维非静力可压缩数值区域预报模式 ARPS(V5.2)。

Numerical simulation for this event is performed using the non-hydrostatic, two-way interactive, triply nested grid mesoscale model MM5 and the structure of the inverted typhoon trough is studied. Furthermore, the formation and development mechanism of the inverted typhoon trough as well as the meso-scale vortex are discussed through the budget of vorticity equation.

采用双向三重嵌套网格非静力模式MM5对这一过程进行了数值模拟，研究了台风倒槽的中尺度结构特征，并通过涡度收支探讨了台风倒槽及中尺度低涡发生发展的物理过程。

Lists can be nested (i.e., items of lists can, in turn, be lists), but it takes some care to unnest them when we need to reach for an item

List能够被嵌套（也就是说，list的条目能一次被列出），但是当我们需要伸手获得一个条目的时候，它需要关注解开嵌套。

Summary In copyright infringement cases,if the alleged infringer uses or even plagiarizes the copyrighted works or parts thereof,which causes no substantially adverse effect on the normal exploitation of the work,nor does it inflict any substantial damages to the right owner,the de minis doctrine should be applied to exempt the liabilities of the defendant and seek the balance of interests between the right owner and the general public embodied by the copyright law.

在侵犯著作权案件中，如果被控侵权行为人虽未经著作权人的同意，使用甚至抄袭了受著作权保护的作品或作品的片段，但若情节轻微，未对该作品的正常使用产生任何实质不利的影响，亦未对权利人的权利造成实质性的损害，则应适用&不计琐细原则&，免除行为人的责任，以寻求在权利人利益与著作权法所体现的社会公共利益之间的平衡。

This new product will become our best seller.

这个新产品会成为我们最畅销的产品。