查询词典 nested

The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物，且所有试材的扩增结果一致，均无目的条带以外的产物扩增，说明Ty1-copia类逆转座子在苹果属植物中广泛存在，且存在历史可能较为久远；从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066)，结果表明，同一基因组来源的这些序列存在高度的异质性。

Objective To develop a nested-real-time PCR method for the detection of FOXP3 mRNA in human peripheral blood mononuclear cells.

目的 建立检测人外周血单个核细胞中FOXP3mRNA的方法。

We examined 24 nonsynonymous SNPs in 13 genes inoled in the one-carbon metabolism pathway in relation to the risk of colorectal cancer in a case-control study nested in the Nurses' Health Study and the Health Professionals Follow-up Study cohorts.

我们检验了一碳单位代谢途径中13种基因的24种非同义替代SNP与直肠结肠癌发生风险的相关性。该病例对照研究是护士健康研究以及卫生专业人员随访队列研究的一个子项目。

Methods Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用套式聚合酶链反应方法对5例艾滋病患者血浆HIV-1 env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及核苷酸序列进行分析，并计算其核苷酸同源替换率与非同源替换率的比值。

Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用巢式PCR方法对5例艾滋病患者血浆HIV-1env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及氨基酸序列进行分析，并计算其氨基酸同源替换率与非同源替换率的比值。

Methods We performed a nested case–control study within the Calcium for Preeclampsia Prevention trial, which involved healthy nulliparous women.

我们在纳入健康未产妇的钙预防先兆子痫临床试验中进行了一项嵌套病例对照研究。

The investigators examined fetal outcome data from 972 pregnancies collected from 1997 to 2002 in a nested case-control study. The participants were nulliparous and had no known medical complications. Pregnancies were grouped in eight categories based on the presence or absence of combinations of hypertension, hyperuricemia, and proteinuria.

研究者检验自1997年至2002年一项病例控制试验中972次怀孕的妊娠结果，受试者皆为第一次怀孕，而且没有已知的其他疾病；根据高血压、尿酸过高、与蛋白尿的有无，将怀孕分为8个项目。

It does not assume automatic parallelization, but instead relies on nested openmp parallelization.

它不承担自动并行化，而是对OpenMP的并行嵌套依赖。

Methods 2-3 days old inbred Balb/C suckling mice were inoculated intracerebrally with tissue suspension of soft parate petechia of HFRS patients and the HFRS virus RNA was tested in brain, lung and kidney tissues of sucking mice by nested PCR.

2～3天的近交系Balb/C乳鼠，脑内接种早期HFRS重症患者软腭粘膜出血点组织悬液，用套式聚合酶链式反应检测乳鼠脑、肺及肾组织中的HFRS病毒RNA。结果接种乳鼠发病死亡，剖检显示

HEV-RNA was detected by nested PCR. And positive PCR products were sequenced and analyzed phylogenetically with neighbour-joining method.

对深圳市采集的80份猪粪便标本和6份戊型肝炎病人血清，提取病毒RNA，然后进行PCR检测，阳性时对扩增片段进行基因序列测定，并进行基因型分析。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。