查询词典 nerve cells

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Materials and Methods:MRI findings of 183 cases with cranial nerve tumors including 135 acoustic nerve tumors,35 tregemimal nerve tumors, 3 olfactory nerve tumors, 3 optic nerve tumors, 3 hypoglossal nerve tumors, 2 vagus nerve tumors, 1 facial nerve tumor and 1 glossopharyngeal nerve tumor verified by operation and pathology were analyzed. Results: Of the cranial nerve tumors,177 were benign tumors,6 were malignant tumors.

材料和方法：收集了183例资料完整，均经手术和病理证实的脑神经肿瘤病例，其中嗅神经肿瘤3例，视神经肿瘤3例，三叉神经肿瘤35例，面神经肿瘤1例，听神经肿瘤135例，迷走神经肿瘤2例，舌咽神经肿瘤1例，舌下神经肿瘤3例。

Most of cases had following characteristic MRI findings: olfactory nerve tumors were often located at anterior cranial fossae and appeared as lobulated mass; optic nerve tumors presented as nerve thickening; tregemimal nerve tumors usually grew across the middle and posterior cranial fossae and appeared as a collar-button appearance; tumors of Ⅷ nerves were nerve thickening and located at CPA; facial nerve tumor was located in mastoid with irregular shape; hypoglossal nerve tumors, vagus nerve tumors and hypoglossal nerve tumors were often located in an enlarged jugular foramen.

脑神经肿瘤发生在特定部位，大部分有特征性MRI表现：嗅神经肿瘤多位于颅前窝，呈分叶状；视神经肿瘤多有神经束增粗；三叉神经肿瘤骑跨颅中、后窝生长，呈哑铃状；听神经肿瘤为第Ⅶ、Ⅷ神经束增粗，并与桥小脑角区肿瘤相连；面神经肿瘤位于乳头内，形态不规则；舌咽、迷走、舌下神经肿瘤位于颈静脉孔区，往往伴有颈静脉孔扩大。

Neural stem cells have a strong self-renew mechanism and it can transform after a little break. Neural stem cells have a long term survival, which mean that it has more probability of wrong copy than mature cells. These cells are formed glioma stem cells in the end. The genes who adjust neural stem cells can express in glioma stem cells, which hold out glioma stem cells from neural stem cells. There is another presume that glioma stem cells come from differentiated cells. Through the gene break of these cells, they can obtain characteristics of stem cells, then form glioma stem cells.

神经干细胞具有很强的自我更新机制，获得较少突变即有可能恶性转化，而且干细胞存活时间较长，这意味着干细胞比成熟细胞发生细胞复制的错误几率更大，因外界环境的刺激而发生突变的机会更多，最终形成脑胶质瘤干细胞，同时调节神经干细胞增殖和自我更新的基因在脑胶质瘤的脑胶质瘤干细胞中也表达，这也是支持神经干细胞是脑胶质瘤干细胞来源的；也有推测认为它可能起源于已分化的细胞，由这些细胞突变发生去分化得来，并通过基因突变而获得了干细胞自我更新的特性，从而形成脑胶质瘤干细胞。

Results: The numbers of Bombesin positive pulmonary cells, the lamina propria S-l00 protein, neuron-specific enolase positive nerve fibers, IgE positive cells, mast cells and IgE positive mast cells significantly increased in bronchiectasis. The changes of pulmonary endocrine cells, nerve fibers and IgE positive cells were more significantly in hyperplastic BALT areas. The S-100 and NSE were found in lymphoid tissue and BALT. A close contact was found between mast cells and the S-100 positive nerve fibers. An IgE positive outer zone was found on MC surface. Mast cells and IgE positive cells were seen in the bronchial epithelium and alveolar septa.

结果：支气管扩张症中，支气管上皮蛙皮素阳性细胞、固有膜S-100蛋白和神经特异性烯醇化酶阳性神经纤维、IgE阳性细胞、MC和IgE阳性MC均显著增多，且在支气管相关淋巴组织增生的区域上述肺内分泌细胞、神经纤维和IgE阳性细胞增多尤为显著，S-100蛋白和NSE阳性神经纤维分布於弥散淋巴组织和BALT中，MC与S-100蛋白阳性神经纤维紧密接触，MC表面有IgE阳性环状带，MC和IgE阳性细胞出现在支气管上皮间和肺泡壁。

The results indicate that the longitudinal nerve fibers in cerebral ganglia and a few of small cells in the surface layer of cerebral ganglia present NOS positive reaction. Abundant NOS positive small cells are in the surface layer of pedal ganglia, and abundant transverse positive nerve fibers in the center of pedal ganglia. A large number of transverse positive nerve fibers are in the center of visceral ganglia; abundant positive small cells and nerve fibers are in two anterior lobes; a few of positive small cells and many encircled positive nerve fibers are in the posterior lobe; a large number of radiate positive nerve fibers are in the lateral lobes.

组织化学显示，存在NOS的部位如下：脑神经节内纵行的神经纤维和表层的少量小细胞；足神经节表层的大量小细胞，中央大量水平分布的神经纤维；脏神经节中部大量水平分布的神经纤维，前叶内大量小细胞和神经纤维，后叶内少量小细胞和许多环行神经纤维，侧叶内大量似放射状分布的神经纤维；脑足和脑脏神经索内的神经纤维。

In recent years, the company has successfully developed some special production lines for manufacturing ordinary batteries, namely, D size, Size C, and Size AA. Now running in the workshop is another assembly line for carbon rod processing machinery. For the past decade, it has introduced most advanced manufacturing techniques and control techniques from abroad, and therefore it has managed to develop automated machines for producing alkaline batteries. The automatic assembly lines that it possesses are: Lines LR6 and LR03 for 200 cells per minute, 300 cells per minute, 400 cells per minute, 600 cells per minute and 1000 cells per minute, Lines LR20, LR14 and LR61 for 150 cells per minute and 300 cells per minute. Powder spraying conducting film, machines for negative electrode calamine cream, diaphragm machines with a speed of 30 cells per minute; negative electrode electric welding machines with a speed of 200-300 cells per minute; current collector assembling machines with a speed of 200-400 cells per minute; battery tray fillers with a speed of 200-400 cells per minute, insulating ring assembling machines with a speed of 300 cells per minute; electroscope machines, 4-cell furling machines with a speed of 300 cells per minute.

公司先后开发了普通电池生产线：一号、二号、五号电池流水线和普通碳棒加工机械，特别是近十年来公司吸收并消化了国外最先进的生产工艺、控制技术，研制开发了国内最先进的自动化碱性电池机械，有200只/分钟、300只/分钟、400只/分钟、600只/分钟、1000只/分钟的LR6、LR03电池自动化生产流水线；有150只/分钟、300只/分钟的LR20、LR14、LR61电池生产线；有正极拌粉系统喷导电膜设备、负极拌锌膏；有30只/分钟的卷隔膜套机，200-300只/分钟负极钉高速点焊机，200-400只/分钟的集电体高速组装机，200-400只/分钟的电池装盘机，300只/分钟的绝缘圈组装机，300只/分钟的验电机和四节缩机等。

The morphology, size and signal of nerve root, nerve ganglion, nerve trunk and femoral nerve, were observed. The brachyaxis and nerve-muscle signal ratio of right L4 nerve root, nerve gatiglion and nerve trunk were measured.

正常组观测腰2~5神经根、神经节及神经干、股神经的形态、大小及信号；GBS及CIDP组观察神经及其周围的改变，测量右侧腰4神经根、神经节、神经干及股神经的短轴径线及神经肌肉信号比。

In group A, the medial antebrachial cutaneous nerve was cut 5 mm away from its origin and its proximal end was anastomozed end-to-end to the distal end of musculocutaneous nerve. In group B, the musculocutaneous nerve was cut 5 mm away from its nerve entry point and the proximal end of medial antebrachial cutaneous nerve were anastomozed end-to-end to the distal end of musculocutaneous nerve. In group C, medial antebrachial cutaneous nerve and musculocutaneous nerve were cut, without further anastomosis.

每组大鼠左上肢制备实验模型，右上肢为正常对照，不作任何处理。A组：于距前臂内侧皮神经起点5 mm 处切断该神经，将其近端与切断的肌皮神经远端行无张力端端吻合；B 组：于距肌皮神经入肌点5 mm 处切断该神经，将其远端与切断的前臂内侧皮神经近端行无张力的端端吻合；C 组：切断肌皮神经和前臂内侧皮神经后不予吻合，形成肱二头肌失神经支配模型。

METHODS: Nerve conduction velocity,wave amplitude and corresponding muscular needle electrode electromyogram of median nerve,ulnar nerve,interior and exterior cutaneous nerve in forearm,suprascapular nerve,axillary nerve,musculocutaneous nerve,radial nerve were tested by keypoint myoelectricity evoked potential equipment made in Denmark.

采用丹麦产keypoint肌电诱发电位仪检测正中神经、尺神经、前臂内、外侧皮神经、肩胛上神经、腋神经、肌皮神经、桡神经传导速度、波幅及相应肌肉针电极肌电图。

He think the world care little whether a man is a churchman, so long as he is good and true.

他想只要一个人慈善忠厚，谁也不会介意他是否是个教徒。

Aiming at the temperature control requirement of multi-workplats fritting furnace, this paper presents an electric circuit design.

针对多工位扩散炉的温度控制要求，介绍了以TCA785为核心的可控硅移相触发电路设计。