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Firstly, the recombinant engineering technology related vaccine preparation was set up, including: 1. the gene knock-out based on linear DNA target molecular in vivo; 2. the selection and counterselection technology of exogenous gene knock in definite site on chromosome; 3. the Gap-repair clone technology in vivo; 4. the recombination technology of single-chain DNA and the overlapping primer; 5. Set up the new selection and counterselection technology of neo-E.

本研究首先建立与疫苗制备相关的重组工程技术包括:①基于线型DNA打靶分子的大肠杆菌体内基因剔除技术,②选择与反选择外源基因定位敲入染色体上特定位点技术,③Gap-repair体内克隆技术,④单链DNA重组技术和重叠引物介导的DNA重组技术,⑤建立&抗生素-酶切位点&选择反选择技术新技术。

Result In the HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT, expressions of hTERT, Bcl-2 and mitochondrial cyt C were significantly down-regulated, while Bax and cytoplastic cyt C were obviously up-regulated, and active caspase-9 was found in addition to procaspase-9 compared with that in negative control cells and untransfected cells.

结果 与未转染细胞和对照细胞相比,转染细胞hTERT、Bcl-2和线粒体cyt C表达明显减少,Bax和胞质cyt C表达明显增加;未转染细胞及对照细胞仅见约46kD的前caspase-9条带,转染细胞可见46kD前caspase-9条带和35kD caspase-9 p35亚基条带。

Methods Westernblot was employed to detect the intracellular expressions of caspase-9, hTERT, Bcl-2 and Bax, and mitochondrial and cytoplastic cytochrome C in HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT.

收集第20代hTERT基因RNAi真核表达载体论定转染细胞、对照细胞及未转染HepG2细饱,采用Western blot检测线粒体,胞质细胞色素C的表达及细胞内caspase-9、Bcl-2、Bax和hTERT的表达。

Satie' s works are of style of Impressionism, Neo-Classicalism, and so on; He had cooperated with artists from otherschools of art actively -such as Cubism, Surrealism, Dadaism .

总的来说,萨蒂的音乐创作体现了新古典主义音乐风格与达达主义精神的混成。

Its main theory source of course is neo-confucionism, dividingly, confucianism, Xing-confucianism. Qi-confucianism and west science had provided conducive thinking element. Confucianism was its spiritual source, Xing-confucianism is its inversal supplement.

其主要的理论渊源当然是程朱理学,但除此之外,先秦儒学、心学、气学以及西学都为清初朱子学理论的完成提供了有益的思想元素。

The known factors that can influence the recurrence and metastasis of operable breast cancer can be divided into two groups. One group of the factors are the clinical and pathological factors, which include age, pathological type, histological and nuclear degree, size of tumor, lymph node status, hormone receptor status, operation mode, adjuvant chemotherapy, endocrinotherapy, radiotherapy and newcome biotherapy, neo-adjuvant chemotherapy and endocrinotherapy.

目前已知的影响可手术乳腺癌术后复发转移的因素可分为两类,一类是临床病理因素,包括患者年龄、肿瘤病理类型、组织学分级和核级、肿瘤分期(包括肿瘤局部情况和区域淋巴结状态)、激素受体状态、手术方式、术后辅助化疗、内分泌治疗、放射治疗以及近年来新出现的生物治疗、新辅助化疗、新辅助内分泌治疗等。

The magmatic activity of collision granites provides the information about the tectonic dynamics from the continental margin epicontinental of the south of Erguna block in the Neo-proterozoic to Early Cambrian.

碰撞型花岗岩的岩浆活动提供了该区新元古代-早寒武世额尔古纳地块南部大陆边缘陆缘增生的构造动力学信息。

From the beginning, his characters have the strong expressionistic style and visual tension and apart from the college-style neo-classical realism which was prevalent in art of China.

他的人物形象从一开始,就具有强烈的表现主义风格的情绪和视觉张力,与当时在中国画坛盛行的学院式新古典主义写实画风拉开了距离。

The change of attitude towards marriage and family is the core of feministic thought in the period of Neo-Cultural Movement.

婚姻观和家庭观的变化是新文化运动时期女性主义思潮的核心。

It is through the many factors above that work together to stimulate the rise and flourish of feministic thoughts in that period of Neo-Cultural Movement.

正是这多重因素共同促成了女性主义思潮在新文化运动时期的蓬勃兴起。

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相关中文对照歌词
Neo Nothing - Post Of All
Sweet Neo Con
Sanso Gateun Neo (Love Like Oxygen)
Neo Animyeon Andoeneun Geol (Romantic)
推荐网络例句

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。