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Virulence Difference of Biocontrol Bacteria J352 to Diffenent Nematodes WANG Yuan-yuan, DUAN Yu-xi, WANG Xu, CHEN Li-jie (Plant Nematology Laboratory, Shenyang Agricultural University, Shenyang 110161, China) Abstract: A bacterium J352 with antagonism to several pathogens was obtained from rhizosphere of cucumber. The effects to different nematodes were test by strain J352 fermentation broth with different concentration.

生防细菌J352对不同种类线虫的毒力差异王媛媛，段玉玺，王旭，陈立杰(沈阳农业大学植物保护学院线虫学研究室，沈阳 110161)摘要：从黄瓜根际分离获得一株对多种病原真菌有较强抑制作用的细菌，用该细菌不同浓度的发酵液测定了对4种线虫的作用。

The result of double staining assay showed that the pine wood nematodes secreted some solute proteins through secretion pore, and cellulase was secreted from stylet that was the same as other plant nematodes.

双重染色分析的结果表明：松材线虫主要通过分泌孔向体外分泌大量的分泌蛋白，而纤维素酶的分泌方式与其他植物线虫相似，是通过口针向外分泌。

The results showed that the quantities of plant parasitic nematodes of Aphelenchoides and Aphelenchus increased at a speed of geometric order after one growing season,which indicated that the two kinds of nematodes had highly potential risk.

研究结果表明栽培前种球内的优势植物线虫——拟滑刃属和滑刃属线虫数量在百合一个生长周期结束后均以几何级数的速率增长；栽培前检出数量极少的垫刃属线虫，生长周期结束后却大量出现；栽培前少量检出的短体属、矮化属、根结属、丝尾垫刃属和伪垫刃属的线虫，周期结束后，均未再检测到。

Structure index was significantly (P.05) different among three plant species whereas channel index and enrichment index were not. Plant species not only modified abundance of plant-feeding, omnivorous and predatory nematodes, but also percentage of each trophic group. Bacterivorous and fungivorous nematodes were affected most by the differences in plant species.

研究表明在沙地生态系统中，3种植物根际土壤线虫群落的多样性、丰富度、均匀度和线虫总数虽然都存在一定的差异，但不显著；功能多样性指数中结构指数存在显著差异，而通道指数、富集指数不存在显著差异；3种植物对根际土壤线虫群落中各营养类群线虫的比例有显著影响。

The 111076 nematode individuls were classified into 43 genera and 4 trophic groups. Among them, 32 genera of nematodes belong to beneficial un-plant parasitic (bacterivorous, fungivorous, omnivorous/predatory) nematodes and 11 genera of nematodes belong to baneful phytophagous nematodes which accounted for 1/4 of nematode collectivity, especially the nematodes in the genus of Tylenchus and Filenchus, involved 51 (91%) and 42 (75%) species of flowers, repectively.

经鉴定111076条线虫分属43个属，4个营养类群（食细菌、食真菌、杂食/捕食和植物寄生线虫），其中有益的非植物线虫属32个，有害的植物线虫属11个，占总调查属数量的1/4，相对比例较大，特别是垫刃属和丝尾垫刃属的线虫出现的频率较高，涉及花卉51种和42种，分别占调查花卉总数的91％和75％。

The result indicated the basic culture had poisonous effect to nematodes, and significant virulence to different nematodes was detected, the sequence of virulence to nematodes was Caenarhabditis sp., Heterodera glycines, Meloidogyne hapla, Ditylenchus destructor.

结果表明，该发酵液呈微碱性，不同浓度发酵液对各种线虫均有较好的防治作用，并且毒力作用具有明显差异，对4种线虫的毒力大小顺序分别为小杆线虫、大豆胞囊线虫、根结线虫和腐烂茎线虫。

The team will train in the cells and nematodes on trial, they found that nematodes Nosema is the natural host.

该研究团队将会在培植细胞和线虫上进行试验，他们发现，线虫是微孢子虫的天然宿主。

Method］Based on perineal striae,root-knot nematodes of greenhouse's vegetable in Luoyang were identified and toxicities of four kinds of fungicides to the root-knot nematodes were tested.

方法］采用传统的制作会阴花纹的方法，对洛阳温室蔬菜根结线虫的种类进行鉴定，并选择4种药剂对根结线虫的二龄幼虫进行室内毒力测定。

Trichosanthes kirilowii in Qianshan County , Anhui Province. Nematodes were extracted from soil around theroot system to a depth of 450 mm using a Baermann funnel and roots were examined for sedentary nematodes. Of the

为了有效防治栝蒌根结线虫，调查了安徽省潜山县栝蒌根结线虫及其发生，并分析了栝蒌根围土壤线虫的组成。

Some strains of the nematode-bearing bacteria were bioassayed for their parasitism. All these bacteria can totally not grow or grows badly on the media incorporating the extracts of pine trees, indicating some substances existing in the trees will harmful to the bacterial strains, which is further convinced by preliminary inoculation showing that single bacterial species will not infect Pinus thugbergii. Hence, the growth and reproduction of the nematode-carrying bacteria, which could produce toxic substances causing the death of the tree cells, is closely related to the nematodes, when they first enter to the trees. In the same time, the activity of bacteria improved the circumstances for which nematodes are living, which in turn will beneficial to the growth of nematodes. Thus, there is a complicated interaction among nematodes, bacteria, and pine trees.

对松材线虫携带的细菌的部分菌株进行了寄生性测定，表明这些细菌在添加松树提取液的细菌培养基中不能生长或基本不能生长，这说明松树体内可能存在某些不利于细菌生长的物质，同时初步的接种试验证实，单独的细菌接种不能感染健康的黑松，因此，线虫携带的致病细菌在最初进入树体时，其生长和繁殖与线虫密切相关，结合细菌产生的毒性物质可造成黑松细胞死亡的情况，说明细菌的生长与线虫有关，同时，细菌的活动改变了线虫在松树内的生长环境，使环境变得有利于线虫的生活，因此，线虫、细菌和松树三者存在着复杂的生物关系。

My first article for Screen Power Magazine, which is to this day is the official magazine of Jackie Chan, was a DVD review of the Hong Kong actioner Gen-X Cops, starring Nicholas Tse and Daniel Wu.

我的第一篇文章为屏幕力量杂志，在对这天是Jackie Chan 正式杂志，是香港actioner Gen X 警察的DVD 回顾，担任主角Nicholas Tse 和丹尼尔吴。

His lips grazed hirs, Now what are you going to do?

他的唇使吃草了 hirs，现在你将要做什麼？