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The first water-wheel link-work escapement clock of which we have adequate records was that made by I-Hsing and Liang Ling-Tsan about +720, so that would make nearer seven centuries than two.

现有足够资料证明的第一个水轮联动擒纵机构，是一行和梁令瓒在公元720年前后制成的。因此，差距就不是两个世纪，是将近六个世纪。

The first water-wheel link-work escapement clock of which we have adequate records was that made by I-Hsing and Liang Ling-Tsan about 720, so that would make nearer seven centuries than two.

何绍庚次审译文：现有足够资料证明的第一个水轮联动擒纵机构，是一行和梁令瓒在公元720年前后制成的。因此，差距就不是两个世纪，是将近六个世纪。

And since there is a possibility that the Chinese method was used by Chang Heng in the Later Han period, the priority of the Chinese water-wheel link-work escapement over tile verge-and-foliot escapement of Europe might well be nearer thirteen centuries than seven.

由于东汉张衡有可能采用过类似的方法，因而，中国的水轮联动擒纵机构，就有可能领先于欧洲的立轴横杆式擒纵机构近十二个世纪。

Our dwellings, low, weathered, mossed, abhorring the lifeless line― the definite, the finite, the aloof― loving rondures and an upward stroke, the tilt of a roof like a boat always aware of the elements in which it is swinging― most fittingly my home was set a hemisphere apart, so far over the globe that to have gone on would have meant to go nearer not farther.

我们的住房低矮，饱经风霜，长满苔藓，憎恶毫无生气的线条──确定、有限、冷漠之物，喜爱回旋曲和向上的笔触。屋顶像船似地翘起，总忘不了兴风作浪的自然力。我的家乡恰好距离这里半球之遥，再往前走便意味着缩短而不是加大距离。

Nearer the rough grass, with its long, brownish stalks all agleam, was full of light.

近处，蓬乱的野草上也洒上了一层光泽，带点棕色的长长的茎杆微微闪亮。

4Every suitable opportunity should be used to bring nearer the peacefulreunification of:r:aiwan and the mainland.

应当利用一切机会促进台湾和大陆的和平统一。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Truth nearer, is more difficult to explain, also more is inescapably involved.

真相越近，越难解释，也越不能自拔。

He thought, and he drew nearer to the ivied archway, and the still water-pools, coldly grey in the twilight.

他心中想道，这时他更加走近长春藤的拱廊以及在暮色中呈苍白寒光的静静池水了。

Johannine reflection moves even nearer than Paul's toward incorporeal immortality.

johannine反射动作，甚至在临近比保罗的，对无形资产的不朽。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。