查询词典 nanogram

The monoclonal antibody was produced by the method of inoculating BALB/c mice intraperitoneally with the hybridoma cells, and purified with protein A-Sepharose 4B affinity chromatography.3 Establishing of ELISA protocol to detect hBLySTwo kind of different ELISA protocols were tried to study the experiment condition of detecting hBLyS level. Streptavidin was used to indirect coating in protocal 1, but biotin-avidin system was used to enlarge the final reaction signal in protocal 2. The optimal reaction condition was chosen by the experiments. The sensitivity of protocal 1 was poor, but the linear relation of protocal 2 standard curve was excellent when the concentration of hBLyS was between 3.2~400 ng/mL. So a protocal was established which sensitivity can reach to nanogram.

建立检测hBLyS蛋白含量的ELISA方法选用2种ELISA方案对hBLyS蛋白含量的测定方法进行研究，方案一用链霉亲和素做间接包被，方案二用生物素-亲和素系统做终反应放大；优化选择2种检测方法的反应条件；方法一的敏感度较低：方法二在hBLyS蛋白浓度为3.2～400 ng/mL范围内，标准曲线的线性关系良好；从而建立了一种可检测hBLyS蛋白浓度为ng级水平的ELISA方法。

Nucleic acids were determined at nanogram levels based on their enhancement effects on the fluorescence reaction system between H 2O 2 and p hydroxyphenylacetic acid catalyzed by Mn [tetrakis(3 N methylpyridiniumy)porphyrin],a mimetic peroxidase.

痕量核酸对meso 四(3 N 甲基吡啶基)卟啉合锰催化H2 O2 氧化对羟基苯乙酸的荧光反应产生增强作用，且增强程度与核酸浓度之间有良好的线性关系，据此建立了测定核酸的高灵敏的荧光分析方法。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。