查询词典 mutation

No disease-causing mutation in MYH-7 gene was found in patients with DCM.

这是在中国HCM患者中首次发现Arg723Gly突变。

If the mother of the proband is a carrier , the chance of transmitting the disease-causing mutation in each pregnancy is 50%.

如果先证者的母亲是携带者，则每次怀孕传递致病突变的几率是50%。

Males with KS1 who are fertile will pass the disease-causing mutation to all of their daughters and none of their sons.

可生育的男性KS1患者将把该致病突变传递给他的所有的女儿，不传递给他的任何一个儿子。

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你是 进化不完全的生命体，基因突变的外星人，幼稚园程度的高中生，先天蒙古症的青蛙头，圣母峰雪人的弃婴，化粪池堵塞的凶手，非洲人搞上黑*的后裔，阴阳失调的黑猩猩，被诺亚方舟压过的河马，新火山喷发口，超大无耻传声扩音喇叭，爱斯基摩人的耻辱，和蟑螂共存活的超个体，生命力腐烂的半植物，会发出臭味的**人，&唾弃&名词的源头，每天退化三次的恐龙，人类历史上最强的废材，上帝失手摔下来的旧洗衣机，能思考的无脑袋生物，损毁亚洲同胞名声的祸害，祖先为之蒙羞的子孙，沉积千年的腐植质，科学家也不敢研究的原始物种， 10倍石油浓度的沉积原料，被毁容的麦当劳叔叔，像你这种可恶的家伙：只能演电视剧里的一陀粪，比不上路边被狗洒过尿的口香糖，连如花都帅你10倍以上，找女朋友得去动物园甚至要离开地球，想要自杀只会有人劝你不要留下尸体以免污染环境，你摸过的键盘上连阿米吧原虫都活不下去，喷出来的口水比SARS还致命，装可爱的话可以瞬间解决人口膨胀的问题，耍酷装帅的话人类就只得用无性生殖，白痴可以当你的老师，智障都可以教你说人话，只要你抬头臭氧层就会破洞要移民火星是为了要离开你，如果你的丑陋可以发电的话全世界的核电厂都可以停摆，去打仗的话子弹飞弹会忍不住向你飞，手榴弹看到你会自爆，别人要开飞机去撞双子星才行而你只要跳伞就有同样的威力，你去过的名胜全部变古迹，你去过的古迹会变成历史， 18辈子都没干好事才会认识你，连丢进太阳都嫌不够环保反正横竖一句话：别让我再看见你，要是见着了你，我一定要把你灭了！

While doing this job, the inner battery of the earth will run out again and another cycle of destruction followed by creation and mutation will start anew.

尽管做好这项工作，内部电池的地球将再次和另一个周期的破坏之后，将建立和突变重新开始

Paper [1]points out that GA with proportionalreproduction,adaptive crossover and mutation probabilitymeets the condition of elitist preserved GAand concludes AGAs global convergence from EGAs global convergence conclusion.At the same time,its considered the Markov chain AGA generates is inhomogeneous.

文[1]指出，具有比例复制和自适应交叉、变异操作的遗传算法满足最优保存GA的条件，则由EGA全局收敛的结论得出AGA也是全局收敛的；同时认为，AGA构成的Markov链为非时齐的。

Mutation:a sudden departure from the parenttype in one or more heritable characteristics, caused by a change in agene or a chromosome steppe:the vast grasslands, esp.

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So compound mutation isan effective method for genetic breedings.

因此，复合诱变是遗传育种一种有效的方法。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。