查询词典 mutation

On the other hand,the Inverse PCR combined with a tail-to-tail primer design requires T4 DNA ligase enzyme to ligate PCR products,which makes the positive rate relatively low.We aim to find out an easy and effective method to perform gene deletion, insertion and substitution mutation.

近几年，有文献报道使用基于Quikchange原理的方法来制作DNA片段的插入、替代以及删除突变，但由于QCM-PCR的低产量，难以产生阳性克隆。

In order to reduce the non-specific binding between oligonucleotides and bases mutation caused by the complicate secondary structure of DNA and excessive PCR amplification, a frequently phenomenon in one-step gene synthesis, we used a two-step method including assembly PCR and digestion-ligation step to synthesis Aspergillus niger lipase gene lipA.

本研究主要针对一步法长片段基因合成中复杂结构的非特异性配对和过多的PCR引入碱基错配等问题，采用二步法(组装PCR和酶切-酶连)合成了黑曲霉脂肪酶基因lipA。

In a randomized, placebo-controlled, double-blind, crossoer study, 24 subjects with type 2 diabetes and one subject with partial lipodystrophy and diabetes due to dominant-negatie mutation in the peroxisome proliferator-actiated receptor- gene (P467L) receied placebo and rosiglitazone for 3 months.

在一项随机、安慰剂对照、双盲、交叉的实验中，24例2型糖尿病和1例因过氧化物酶增殖体激活受体基因(P467L)显性负向突变导致部分脂肪营养不良和糖尿病的患者接受了3个月的安慰剂和罗格列酮治疗。

The mutation rates were 30% and 25% in LBC and FM,respectively. It was \ mation of infinite life span human cell strain by transfection with v-Ki-ras.Oncogene,1990;5:1415. 6\ Yang D,Louden C,Reinhold DS,et al.Malignant transformation...

这一结果表明p53基因突变在大肠癌中的普遍存在，并在大肠癌的发生过程中起着重要作用；而家族成员中发现p53基因突变将可为癌症的癌前监控和预防提供重要佐证。

Genes that are numbered are essential: a mutation in any one of these loci prevents successful completion of the lytic cycle.

标有数字的基因是必须的，用三个字母缩写表示的基因在通常的感染条件下可认为是非必须的。

Mages have additionally been known to suffer physical mutation: The magister lords of the Tevinter Imperium were widely reputed to have been so affected by their years of lyrium use that they could not be recognized by their own kin, nor even as creatures that had once been human.

据说魔法师们还会因此还会产生物理上的变异：众所周知， Tevinter 帝国的魔法师领主们就因为长期使用这种物质而导致连他们的亲戚都无法辨认出他们的样子，甚至他们的外形都无法再让人们称之为人类。

We show that the melanistic K locus mutation in North American wolves derives from past hybridization with domestic dogs, has risen to high frequency in forested habitats, and exhibits a molecular signature of positive selection.

此研究报告的另一作者安德森表示，数千年以来，人类就已经培育出了黑色皮毛的家用狗。她说：&不过，如今，我们不仅看到野狗也有黑色皮毛，还发现黑色皮毛对狗非常有利。&

OBJECTIVE: To investigate the relationship between the clinical manifestations of a patient with MELAS and a novel mitochondrial DNA mutation.

目的：探讨1例MELAS患者的临床表现和线粒体基因突变的关系。

The probands of microtia pedigrees were showed normal karyotype. Exclusion of vrk1 gene in the mutation of microtia pedigrees.

先天性小耳畸形家系核型正常，初步排除了vrk1基因在先天性小耳畸形发病中的作用。

The optimized microvolume DNA sequencing can detect known and unknown mutation correctly and reliably at the same time.

4优化的微量DNA自动测序技术具有准确可靠的优点，可同时检测已知和未知基因突变。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。