查询词典 mutation

METHODS: We assessed our patients by clinical and electromyographic studies, by intercostal muscle biopsies for in vitro microelectrode analysis of neuromuscular transmission and quantitative electron microscopy EM of 409 end plates, and by mutation analysis, and expression studies of the mutants.

我们通过临床表现及肌电图检查、通过肋间肌活检进行神经肌肉传递的体外微电极分析和对409个运动终板的定量电子显微镜检察，以及运用突变分析和突变体的表达研究对患者进行分析评定。

However,their fluorescence emission intensities decreased by 16-22% and the ellipticity values at the negative trough increased by 13-16% for the mutants,indicating that the conformation of prochymosin was perturbed after mutation.

但它们的荧光强度下降了16-22%，在负谷中的最大椭圆值增加了13-16%，表明突变确实给凝乳酶原的空间结构造成了微扰。

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

Additional, denotative type is carried out to note below policy support endowment recombine appear on the market the likelihood of mutation of existence of company outstanding achievement, this can be brought to investor above quota accrual.

另外，政策支持下实施外延式注资重组的上市公司业绩存在突变的可能，这能给投资者带来超额收益。

A new method to solve the combinatorial optimization problems with mixed evolutionary algorithm based on natural scale enumerative system coding was proposed in this paper. Elitism strategy, one-point crossover and Gaussian mutation were used in the algorithm. An ingenious method for operation among big parameters was designed, which can avoid overflow and simplify the operation.

在自然进位制编码的基础上，算法采用了遗传算法的单点交叉算子和进化规划的高斯扰动算子，并运用了精英保留策略；算法实现时采用逐位运算法实现大数值运算，避免了运算溢出，减少了运算量。

Objective To study the gene mutation in a pedigree with Dowling-Meara type epidermolysis bullosa simplex .

目的研究Dowling-Meara亚型单纯型大疱性表皮松解症一家系的基因突变。

The mutation of G2034R is the underlying cause of epidermolysis bullosa pruriginosa in this family, not common polymorphism.

G2034R突变是引起该家系临床病变的特异突变，不是多态性变化。

Epidermolysis bullosa is normally diagnosed on a combination of the clinical symptoms and family history, with a skin tissue sample and mutation analysis.

大疱性表皮松解症通常是诊断上的结合临床症状及家族病史，与皮肤组织样本和突变分析。

The splicing mutation of COL7A1 gene is the underlyi ng cause of and specific rather than common polymorphism for the family with dys trophic epidermolysis bullosa pruriginosa subtype.

结论COL7A1基因剪接位点的突变是引起该家系临床症状的特异突变，而非多态性改变。

Objective To identify gene mutation of a epidermolysis bullosa pruriginosa family. Methods Polymerase chain reaction, DNA squencing, multiplex PCR using allele-specific oligonucleotide primers.

采用聚合酶链反应，DNA直接测序以及等位基因特异引物的多重PCR方法，对一个痒疹样大疱性表皮松解症家系40人（其中患者23例）进行基因突变情况检测。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。