查询词典 mutants

For investigation of genetic mechanisms of leaf polarity formation, we screened for Arabidopsis mutants with aber...

通过EMS化学诱变，寻找到一些形态相似，叶发育异常的突变体，遗传实验证明这些突变体可以被分成两类。

The mutants in good condition were selected in the culture medium of certain concentration of acrylonitrile.

为获得耐高浓度丙烯腈的菌株，利用紫外线对珊瑚诺卡氏菌进行诱变，并将诱变后生长情况较好的突变株在含一定浓度丙烯腈的培养基中进行筛选。

The main results were listed as follows:(1) Col-0 ecotype and the mutants were inoculated by Bipolaris oryzae, we discovered Col-0 ecotype was resistant to the isolation and vaccinal area emerged no spot; the mutants represented resistant.

将水稻胡麻斑病菌接种到拟南芥野生型和突变体植株上，发现野生型Col-0对病菌表现抗病，接种的叶片上没有任何病斑；而突变体植株则表现出不同程度的抗感差异。

It was typically indirect effect that the following variations induced in the progenies of endosperms implanted by heavy ion beams were discovered. 1 In comparison with control, there were very high differences in biological trait, anti-oxidation enzyme activity, MDA content and protein content, chromosome aberration rate and micro-nucleus rate in M1 generation. 2 Shorter stem variants and purple stem mutants were analyzed by RAPD and AFLP. The analytic results showed that the sequences of their DNA were changed, so those variations were not physiological damage only. And there were differences among M2 generation plants of the same sort of variants or mutants, it was indicated that M2 generation may be in disjunction.

M1代的生物学性状、幼苗的抗氧化酶活性、MDA含量、蛋白质含量和染色体畸变率及微核率等发生了较大的变化。2）对其矮杆变异株和紫色茎杆突变体（M2）做RAPD和AFLP分子鉴定等研究，结果显示了矮杆变异体和紫色茎杆突变体的DNA序列发生了改变，而非仅仅产生生理损伤；同一种变异体或突变体，在M2代单株间也存在着明显的差异，表明它们可能还处于分离状态。

However, these LeuRS mutants can mischarge tRNA〓 isoacceptors, indicating that the editing ability of these LeuRS mutants were impaired to varying extents also.

E292突变后的LeuRS变种酶能够误氨基酰化tRNA〓，表明它们的编校功能受到不同程度的损伤。

The genetic study suggested that the carbendazim-resistance in these mutants could be steadily inherited by asexual and selfed reproduction, and the resistance was controlled by the same single major gene both in laboratory mutants and field resistant isolates, the different levels of resistance or the same resistant level in different strains maybe conferred by mutations at different sites or one site with different allelic mutations. The gene compensates for the sensitivity to MDPC was allelic to that governs the carbendazim resistance, but the mutation for increasing sensitivity to MDPC in this gene could get the pathogen highly resistant to carbendazim.

抗药性遗传研究表明，在所研究的抗药突变体中，抗药性在自交和无性繁殖后代中能稳定遗传；室內抗药突变体和田间抗药菌株对多菌灵的抗药性由同一个主效基因控制，但它们发生突变的位点不同或者同一碱基位点发生了不同的突变；对MDPC的敏感性也是由单个基因控制的，该基因与控制多菌灵抗性的基因是等位基因，当该基因发生对MDPC的敏感性增加的突变时会使病菌对多菌灵产生高水平抗性。

Null mutant Δmnh6 produced appeared offwhite hyphae, showed reduction in growth, conidiation and appressorium formation, and also showed reduction in appressorium turgor pressure. And the hyphae were more readily wettable by a solution containing SDS and EDTA and more easily digested by fungal cell-wall-digesting enzymes. The appressoria produced by Δmnh6 mutants showed diminished host penetration and the infectious hyphae of Δmnh6 mutants showed reduced in planta growth. Targeted deletion of mnh6 resulted in the greatly reduced virulence capacity to barley and rice CO39 in infection assays.

突变子Δmnh6的菌落形态不同于野生型GUY11，菌落呈灰白色，菌丝稀疏，生长速度变慢，产孢菌丝细胞壁的溶剂疏水性消失，菌丝细胞壁容易被细胞壁降解酶降解，产孢减少，孢子形态变得细长，孢子的附着胞形成率降低，附着胞的膨压降低，附着胞对寄主植物表面的穿透能力减弱，侵入后生长速度减慢，喷雾接种后对大麦和水稻CO39的致病性消失。mnh6基因重新引入Δmnh6后，突变子的表型恢复。

Four further single mutants ofN546(N546G,N546D,N546K,N546Δ), three double mutants and a triple mutant were constructed,and toxins were purified by isoelectric precipitation and an AKTA purifier 100 system.When bioassayed against H.armigera using purified toxins,N546ALC_(501.67μg/mLshowed 1.78 times increased toxicity than wide-type Cry1AcLC_(50 2.98μg/mL,and mutant N546D,N546K and N546Δshowed a great loss in toxicity.

对N546进一步突变，获得了突变子N546G、N546D、N546K和删除突变N546Δ及3个双突变和1个三突变，利用等电点沉淀法和AKTApurifier 100系统纯化了毒素蛋白，生测发现N546ALC_(50为1.67μg/mL对棉铃虫毒力比野生型Cry1AcLC_(50为2.98μg/mL提高了1.78倍，而N546D、N546K和N546Δ的毒力则降低了80～90%。

The amount of covalently closed circular DNA in the serum is reported to be higher in patients who develop YMDD mutants than in those without mutants.

据报道，血清中共价闭合环状DNA的水平在发生YMDD变异的病人中要比不发生变异的病人高。

It was also found base transversion had a extremely high ocurrence frequency in the single base substitution mutants when the plasmid was replicated in FL-FEN-1〓 cell or MNNG pretreated FL-FEN-1〓 cell, while in all mutants with multiple base substitution there were at least one transversion mutation taken place.

分析还发现，在FL-FEN-1〓细胞自发突变和非定标突变含有单碱基替换突变的突变子中，碱基颠换的发生率极高，而在FL-FEN-1〓细胞自发突变和非定标突变的多碱基替换突变子中，每个突变子均至少含有一个碱基颠换突变。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。