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The dynamic changes of physiological and biochemical indexes of healthy spikes and inoculation ones of the resistant mutants indicated that PAL activity and content and changing tendency of total phenolate and flavoniods and MDA in the healthy spikes of the mutants were similar to that of their susceptible donors, whereas, for the inoculated spikes, content and changing range of these indexes existed significant difference between two kinds of strains, changing from high-resistant variety (Sumai3 ) to their susceptible donors.

以典型抗扩展品种和突变体供体亲本为抗、感对照，对抗赤霉突变体的健康穗及接种穗的生理生化特性研究结果显示，在健康穗中，抗性突变体的PAL活性、总酚、类黄酮及MDA含量及变化趋势与其感病亲本相一致，但在病原菌侵染后，这些生理生化物质的含量与变化程度与其亲本有了明显的差异，其变化范围介于典型高抗品种和感病亲本之间，变化趋势与亲本或抗性品种一致。

The lifetime of M intermediate of the three mutants in PVA films were recorded by employing photomicrography. In distilled water, the visible absorption maximum of BRI119T/T121S/A126T and BRI119T/T121S/A126T/E194Q mutants were blue shifted notably by 11 and 12 nm respectively and the BRE194Q's was weakly red shifted in comparison with WT-BR.

与野生型BR相比较，在水溶液中，单突变体的可见吸收光谱的最大吸收峰发生了轻微红移，三突变体和四突变体的最大吸收峰则分别发生了11.0和12.0 nm的明显蓝移。

The lifetime of M intermediate of the three mutants in PVA films were recorded by employing photomicrography. In distilled water, the visible absorption maximum of BR(subscript I119T/T121S/A126T) and BR(subscript I119T/T121S/A126T/E194Q) mutants were blue shifted notably by 11 and 12 mm respectively and the BR(subscript E194Q)'s was weakly red shifted in comparison with WT-BR.

与野生型BR相比较，在水溶液中，单突变体的可见吸收光谱的最大吸收峰发生了轻微红移，三突变体和四突变体的最大吸收峰则分别发生了11.0和12.0nm的明显蓝移。

The main results were showed as follows:Through screen and identification of morphology in M_2 and M_3, respectively, the mutants with mutative traits of plant were found. In which, 11 mutations were leaf variations, including light-green leaf, deep-green leaf, durative yellowish leaf, temporary yellowish leaf, inlaid yellowish leaf, wrinkly leaf, smooth-edged curly leaf, splitting-shaped curly leaf, peduncular-shaped leaf, round leaf and larger leaf, 12 were mutations on plant type, including excessive branches, lacking branches, highness, dwarf-like, erect posture, reclinate posture, strong stalk, slender stalk, tufty branches, wrinkled plant, purple stalk and hair-covered plant, 14 were flower variations, including dense flowers, light-yellow petals, white petals, white-mosaic petals, wrinkled petales, back-rolled petals, narrow petals, larger petals, smaller petals, apetalous flowers, variable-numbered petals, fertile pistil protraction, sterile pistil protraction and multi-anther, and 5 were physiologic mutants, including genic male sterile, cytoplasmic male sterile, bud-dead, and early and later bloom.

主要结果如下：通过田间M_2筛选和M_3重复鉴定，在田间农艺性状方面共获得浅绿叶色、深绿叶色、转绿黄化叶、持续黄化叶、边缘黄化叶、皱缩叶、光叶型卷叶、裂叶型卷叶、薹叶形叶、宽圆叶和大叶等11种不同的叶部性状突变体；同时还筛选到多分枝、少分枝、高杆、矮杆、株型紧凑、株型松散、粗茎、细茎、丛生分枝、整株皱缩、紫茎、整株被毛等12种植株性状突变体，密花、浅色花瓣、白色花瓣、白斑花瓣、皱瓣、卷瓣、窄瓣、大瓣、小瓣、无瓣、花瓣数目不定、可育型柱头外露、不育型柱头外露和多雄蕊等14种花器性状突变体，以及细胞核雄性不育、细胞质雄性不育、死蕾、早花和迟花等5种生理性状突变体。

The conidial production on infected sclerotia by the mutants SV-5- 1, SV-5-2, and SV-10-1 were as high as or higher than that by Chy-1 for at least one treatment with these mutants.

除菌株V-250-1外，其它3个突变菌株在至少1个接种处理的菌核上产生分生孢子的能力显著高于野生菌株或无明显差异。

Analysis of double mutants between bro-1(bp133) and several heterochronic mutants showed that the proliferative seam cell division occurred at any stage was defective in bro-1 mutation background.

对bro-1和不同异时基因构成的双突变的细胞谱系分析表明在L2时期以外时期出现的增殖性分裂也可以因为bro-1基因的突变而缺失。

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示：1BP刺激细胞可促进c-Jun活化，并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性，均可明显抑制BP诱导的c-Jun活化，但阻断p38活性对BP引起的c-Jun活化无明显影响，提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化，并降低磷酸化ERK的表达水平，但对磷酸化JNK的表达水平无明显影响，说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加，并同时诱导Akt和ERK的磷酸化水平的升高，表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况，发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加，提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化，降低磷酸化Rb以及E2F1蛋白表达水平，表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高，可见c-Jun也参与调控BP诱导的p53/p21通路活化。

To study the function of this ORF, we constructed Tn5gusA5 insertional and integrational mutants in the corresponding gene of wild type strain 8004 and analyzed the phenotypes of the mutants.

为了研究该σ因子在Xcc中的作用，我们构建了该基因的Tn5gusA5的插入突变体和pK18mob定点整合突变体并进行了表型分析。

Systematic infection of mice is used as a model to test the virulence of several mutants. According with their ability to form hyphae, nonfilamentous mutant is avirulent, and hyphae defective mutants have decreased virulence.

用系统感染小鼠模型分析这些突变株的致病能力，同它们形成菌丝的能力相一致，野生菌的致病能力最强，形成菌丝弱的突变株致病能力也减弱，不能形成菌丝的cph1 efg1突变株和crk1则无毒性。

To determine the regulating role of mtlR in transcription of mannitol PTS operon in V.cholerae,and whether it plays a role in the ferment difference of the toxigenic and non-toxigenic strains,the mtlR deletion mutants from the mannitol rapid-ferment strain(non-toxigenic strain) and slow-ferment strain were constructed.Comparisons of the growth in M9 containing 0.2% mannitol as the sole carbon source and pH change in mannitol fermentation media of these wild strains and their mutants,indicated that mtlR is a repressor.Its repression in mtlCBA transcription was further verified with the analyses of quantitative reverse-transcriptional PCR.

通过比较快发酵菌株和慢发酵菌株mtlR缺失突变株与野生株在含0.2%甘露醇的M9培养液及甘露醇发酵液中生长、产酸等的变化，定性地证明了mtlR基因的抑制作用；另外通过定量RT-PCR进一步验证了MtlR蛋白在mtlCBA转录水平发挥负调控作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。