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The results were as followed: 1 Mutagenesis Breeding of High Tannase Activity Strains and preparing of Tannase As a original strain, Asp. niger CY was mutated by UV treatment. One strain was obtained from the mutants.

全文实验结果如下： 1 单宁酶菌种的选育和单宁酶的制备以一株产单宁酶的黑曲霉为出发菌株，进行了菌株的诱变选育，得到了一株单宁酶高产菌株。

Ansochromogenes was transformed by pUC1169 from itself. 8 mutants lost ability to produce nikkomycin were screened from 4068 colonies of Vio〓 Thio〓 with Tn4560 transposition. Southern blotting suggested that Tn4560 was inserted in at least 4 positions of chromosomal DNA in S.

于39℃，MM／Vio条件下培养携带有pUC1169的7100孢子，Tn4560发生转座；筛选到4068个转座菌落，并从中得到8株尼可霉素阻断突变株；对这8株突变株的总DNA进行Southern杂交分析表明，Tn4560至少在4个不同的位点插入到7100的染色体上。

The subcellular localization of TIAR full length and truncated mutants were observed by using fluorescent microscopy.

酶切鉴定及测序结果显示TIAR全长及缺失体的pEGFP-C3重组表达质粒构建成功。

Subcloning technique was performed to construct the expression plasmids of EGFP-fused TIAR full length and truncated mutants.

结果细胞免疫荧光结果显示在SMCC-7721细胞中TIAR主要浓集于细胞核，细胞浆也有少量弥散分布。

To test the influence of trehalose accumulation on response to freeze stress, we constructed all single and double gene disruption mutants of the trehalase genes ATH1, NTH1 and NTH2 and examined the changes of their trehalose accumulations and their freeze tolerance.

为了检测冷冻压力下海藻糖积累的作用，我们构建了海藻糖酶基因ATH1、NTH1和NTH2的各种单基因突变株和双基因突变株，并对突变株的海藻糖积累情况和耐冷冻能力进行了检测。

Analysis of these mutants revealed that the expression level of the neutral trehalase gene in RNAi mutant (Ma688 strain) was reduced to 42% of which found in wild-type strain and the over-expression mutant (Ma113 strain) showed approximatlely 2-fold higher levels of neutral trehalase mRNA than that found in wild-type control. Then the transformants Ma688 and Ma113 were chosen for closer examination, including trehalose content, storage longevity , thermotol

利用Northern blot 分析这几个突变菌株中性海藻糖酶基因mRNA 的含量，选择中性海藻糖酶基因超表达2 倍和中性海藻糖酶基因被抑制到42%的两个转化菌：Ma113 和Ma688，将其与出发菌株进行分生孢子内海藻糖含量的测定，孢子储藏

Unctional study of the negative selection sites, possible Darwinian positiveselection sites and deletion mutants The aim of this study was to observe the role of negative selection sites,possible Darwinian positive selection sites and deletion fragments in FKN/CX3CR1 binding affinity, chemotaxis and adhesion.

KN／CX3CR1负选择位点、可能存在的达尔文正选择位点及缺失片段的体外功能研究观察负选择位点，可能存在的达尔文正选择位点及缺失片段对FKN／CX3CR1介导的功能的影响，应用定点突变技术构建不同的突变子，体外实验观察突变子对FKN／CX3CR1在亲合力、趋化功能和粘附功能上的影响。

In this thesis, we find that dlg is indispensible in the establishment of anterior-posterior and dorsal-ventral polarity of drosophila oocyte. Removal of Dlg function from the posterior follicle cells using the FLP/FRT system leads to disruption of oocyte skeleton reconstruction that is elicited by the failure of those posterior cells to differentiate normally in mid-oogenesis. We demonstrate that abnormity of Notch, JAK-STAT and EGFR signal pathway in dlg mutants contributes to this aberrant differentiation. dlg null mutant also blocks the normal differentiation of two groups of anterior follicle cell-stretched cell and centripetal cell, but not border cell, with a lower penetrance. However unlike the result in posterior follicle cells, Notch and JAK-STAT signaling are both undisrupted in all mutant anterior follicle cells, implying other fate determinants may be involved.

我们的研究发现，后端滤泡细胞中的Dlg在果蝇卵子发生中期卵母细胞前后轴和背腹轴建立过程中也是必须的，PFC中dlg完全缺失型突变引起PFC的分化异常，导致卵子发生中期卵母细胞骨架重组异常，Stau、Grk等极性决定蛋白定位错误。dlg突变阻碍了Notch、JAK-STAT、EGFR等调节PFC分化的信号通路的激活。dlg突变的PFC也没有获得前端滤泡细胞命运。dlg突变不影响前端滤泡细胞群中边界细胞的分化，但是在一定程度上影响伸展细胞和向心细胞的分化，并且这种影响不依赖于前端滤泡细胞Notch或JAK-STAT信号激活的异常。

In this experiment, methods of artificial inoculation were used to evaluate 17 species (including 26 types), 2 mutants, 3 cultivars, and 4 hybrids of Malus for their resistance to Valsa Mali. After 3 years repeated trials, it showed that 7 of them are highly resistant 2 are resistant, 2 are medium resistant and others are different grades of susceptible resources.

在1983年接种方法研究的基础上，于1985—1987年采用人工田间砸伤接种法，对苹果属17个种的26个类型、2个变种、3个品种和4个杂交种，共35份种质资源材料进行抗腐烂病的系统鉴定，经3年重复，鉴定出高抗级种质资源7份，一般抗2份，中抗2份，其它均为感病各级的资源。

Further studies showed some DH plants could generate two or more than two stems in which wrinkledness mutant and wild types except for the leaf wrinkledness, therefore they were the better materials for studying the wrinkledness characteristic. The finding is useful in studying, locating and cloning the wrinkledness gene linked to leaf mutants of Brassica allosextuple CGMCC № 2553 DH lines in the future.

进一步研究发现，在同一DH再生植株上常常会萌发出皱缩和野生型的2个或多个分枝，由于皱缩和野生型除了叶片皱缩性状不同外，其他性状完全相同，因此叶片突变体和野生型是研究叶片皱缩性状的理想材料，有利于进一步研究皱缩基因的遗传，定位、克隆叶片皱缩相关基因。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。