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Growth of somatic cell mutants MN12-2 and N51 of Pennisetum purpureum and distribution of K(superscript +) and Na(superscript +) at seedling stage under sea-salt stress were studied.

以耐盐象草体细胞突变体MN12-2和对照象草N51为材料，研究了海盐胁迫对其苗期生长及Na、K分配的影响。

But SDS-PAGE and immunodetection indicated that both mutants can not secrete into the periplasm like the wide type protein.

但SDS-PAGE和Western-blot鉴定表明突变体不再能象野生型一样分泌到胞外的间质中。

Through the research we have established in planta transformation system, mutangenesis and screening system for salt sensitive mutants, and constructed a cDNA library under salt stress and cloned a serious of salt-tolerance related genes which had been confirmed the function by overexpression in Arabidopsis or antisense expression in Thellungiella. We also compared different mapping strategy through the analysis of the genetic polymorphism between ecotypes, and establishing mapping population for physical mapping.

建立了小盐芥的转化体系与盐敏感突变体诱变与筛选体系，构建了盐胁迫下的cDNA文库并克隆了一批耐盐相关基因，并通过模式植物拟南芥转化与小盐芥的基因反义表达证实其功能，通过不同生态型小盐芥的遗传多样性分析探讨了基因组作图的策略，建立了作图群体并开始进行物理图谱构建。

The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12 000 independent transformant lines were acquired by the above method, from which two mutants related to plant defense response and development, asr1 (auxin and .salicylic acid responsive 1.) and dai1 (brought tolerant and obscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSK1015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asr1(auxin and salicylic acid responsive 1)和dai1(drought tolerant and abscisic acid insensitive 1)。

Leaf primordia of the as1-101 and as2-101 mutants grew slowly in comparison to those in wild-type.

另外，它们的表达特征也非常相似，都在细胞分裂旺盛的组织中有强烈的表达。

These two mutants were prototrophic type and could cause hypersensitive reaction on tobacco leaves.However,their virulence decreased greatly in comparison with wild type.

经SDS-PAGE电泳，筛选出2个胞外蛋白减少9种的突变株PM2644和PM239，其可以引起烟草叶片典型的过敏性反应，致病力比野生型菌株显著降低，皆为原养型菌株。

For the analysis of the non-kinase activity and the relationship between kinase activity and non-kinase activity, the recombinant full length MLCK was expressed and purified quantifiably with the E.coli expression system. The ATP binding site site-directed mutants of MLCK which lost the kinase activity were acquired.

为了更好地研究MLCK分子的非激酶作用以及激酶与非激酶两者的关系，本实验利用大肠杆菌表达系统大量表达并纯化出完整的MLCK分子，并对其ATP结合位点进行定点突变，获得无激酶活性的MLCK突变体。

In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants.

在这项研究中，我们描述了一种新的战略寿命测定酵母中复制，并应用于筛选突变体的抗前氧化剂。

There were no significant differences （P〉 0.05） between the four mutants and Chy-1 in infection of sclerotia of S.

在没有杀菌剂存在的情况下，这些突变菌株的转代培养后代和感染核盘菌菌核后产生的分生孢子后代仍具有抗性。

In this study, the deletion mutants of SulA with regard to C-terminus were constructed and the interaction with ClpY was analyzed in yeast two-hybrid system.

本实验中，为确认ClpYQ蛋白酶辨认SulA蛋白的区域，建构SulA之C-端不同大小片段缺失的突变蛋白，以酵母菌双杂交系统测试，各个SulA突变蛋白与ClpY之间交互作用的情形，发现ClpY辨识的区域可能位於C-端的高疏水性片段，C-端第20 - 30个胺基酸。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。