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Nit mutants could be selected from chlorate-resistant sectors on Czapek but not on minimal medium . A total of 328 stable nit mutants were selected from 3504 sectors on Czapek and used for the further study. Of these nit mutants, 58. 8% were classed as nit1, 26. 8% as nit3, 13. 8% as NitM, 0.6% as nitD unable to from each isolate were complementally paired in all possible combination.

全部3504抗氯酸盐突变体在Czapek中共鉴定出328个稳定的nit突变体,其中nit1占58.8%,nit3占26.8%,NitM占13.8%,nitD占0.6%。37个菌株中,21个(73%)获得了nit突变体,16个(26%)还没有,仅5个菌株获得了NitM突变体。

In M3 generations, 40 mutants of leaf, stalk, flower, seed and cotyledon were obtained by treatment of 60Go γ rays, and 145 mutants by EMS.

结果在M3代分别获得40份和145份叶、茎、花、种子、子叶等性状变异的材料。

As shown by the result, the biomass and astaxanthin content of oceanic red yeast was reduced due to the presence of β-ionone which inhibited the synthesis of carotenoids. The lethality rate of oceanic red yeast would attained 92.3% if 370 mg/L of the β-ionone concentration was contained in the plate medium. The oceanic red yeast mutated by ethyl methane sulfonate was cultured on plate medium containing 370 mg/L of the β-ionone. 200 mutants were randomly screened, positive mutants with increased the biomass, astaxanthin volumetric yield and astaxanthin specific yield accounted for 18% of the total strain, the mutants with higher biomass, astaxanthin volumetric yield and astaxanthin specific yield higher occupied 22.5%, 45% and 46%, respectively.

实验结果表明,在β-紫罗兰酮存在的情况下,由于类胡萝卜素合成受到抑制,海洋红酵母的生物量和虾青素合成量都减少;当平板培养基中β-紫罗兰酮浓度达到370 mg/L时,海洋红酵母的致死率为92.3%;在这种平板培养基上涂布经甲基磺酸乙酯诱变的海洋红酵母,随机筛选200个菌落,结果表明生物量、虾青素体积产率和细胞产率均有所提高的正突变株占18%,生物量、虾青素体积产率和细胞产率的单项指标有所提高的正突变株分别占22.5%、45%和46%。

Typical late-flowering mutants had more leaves than wild type, but the Atdfc mutants plants contain the same leaf number as the wild type .

Atdfc突变体在营养生长阶段与野生型并无明显差异,但其抽薹的时间要比野生型晚。

Then, the experiment that edelfosine inhibited the phosphatization of Spm1 was carried out in order to elucidate whether edelfosine affect on the phosphatization of Spm1 induced via Mid2 and inhibited via Pmp1.(1)、On the inhibition experiment of parallel growth of S. pombe wild-type cells, spm1 mutants, pmp1 mutants, mid2 mutants and relevant retransform strains, the spm1 mutants grew well treated with 5.0μM and 10.0μM edelfosine for 24 h; the growth ratio of spm1 mutants had a statistic significance in between the cells previously mentioned and the S.

(1)、通过野生型粟酒裂殖酵母和胞质分裂突变体spm1Δ、pmp1Δ、mid2Δ及其再转化株对依地福新的平行生长抵抗试验,进一步确定依地福新对spm1、pmp1、mid2基因的影响;(2)、应用依地福新对Spm1磷酸化的影响试验,阐明依地福新是否通过影响Mid2蛋白和Pmp1蛋白的表达而影响Spm1蛋白的磷酸化。

Histological and cellular research on rachis of healthy and inoculated spikes of the mutants at the different stages showed that, structure and developed tendency of cortical sclerenchyma tissue and green tissue and fibrovascular tissue in the rachis were alike between the mutants and their donors at anthesis stage, which also showed no apparent difference in the inoculated spikes'rachis between the mutants and their donors, major difference was that the mutants had less hyphae number than their donors.

对抗赤霉突变体的健康穗及接种穗的穗轴细胞组织学研究结果显示,抗性突变体与其感病亲本的花期健康穗轴在皮层厚壁组织、绿色组织及维管组织结构上基本一致,不同发育时间的变化趋势也一致;接种处理后,抗性突变体与其感病亲本的穗轴组织在各方面的变化趋势相一致,变化量也无明显差异。

Six different deletion and insertion mutants of BBSV-X were constructed and these were inoculated to Chenopodium amaranticolor. The preliminary results show that the sat-RNA could enhance symptom of BBSV-X and its insertion mutants on Chenopodium amaranticolor, but the pathogenicity of the CP deletion mutants was reduced.

构建了BBSV-X基因组6个缺失和插入突变体,将它们的体外转录物单独或与sat-RNA一起混合接种苋色藜,初步实验结果表明,sat-RNA能增强全长BBSV-X基因组及含全长基因组的突变体对苋色藜所致的症状,但减轻CP缺失突变体的致病性。

After the selection from one to three generations, several mutants were obtained. The major mutants included non-nodule mutants (18 lines) which failed to nodulate after inoculated with rhizobia for two weeks, ineffective nodulation mutants (9 lines) which had less uneven and white or transparent nodules, an abnormal leaf and flower mutant which was dwarf without stipules, abnormal flowers and less nodules, and a slender mutant with a thin stem and smaller leaves.

从种植的三代植株中,筛选到多种共生固氮根瘤的突变体,其中不结根瘤突变体18个株系,表现为接种根瘤菌两周后无根瘤形成;结无效根瘤突变体9个株系,表现为根瘤数目少且分布不均匀,根瘤呈白色,有些为半透明;花叶形态异常突变体1个株系,表现为除根瘤数目少外,植株矮小、托叶消失、花形态异常;纤细突变体1个株系,表现为除根瘤数目少外,植株变小、茎细叶小。

The main work of this dissertation is constructing Arabidopsis gain of function mutants pool, screening mutants related to plant defense response, cloning the genes and studying their functions. The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12000 independent transformant lines were acquired by the above method from which two mutants related to plant defense response and development, asr1 (auxin and salicylic acid responsive 1) and dai1 (drought tolerant and abscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料,以激发标签为技术平台,构建了拟南芥的功能获得突变体库,从中筛选具有抗病、抗逆表型的突变体,克隆基因并进行基因的功能研究,取得的主要结果如下:(1)利用含激发标签质粒pSKI015的农杆菌直接转化拟南芥植株,以抗除草剂的Bar基因为筛选标记,获得了约有12000个独立转化株系的突变体库,并从中筛选到2个与抗病、抗逆和发育相关的突变体asrl(auxin and salicylic acid responsive l)和dail(dought tolerant and abscisic acidinsensitive l)。

Results showed 172 DH plants in 2006, 230 DH plants in 2007 were obtained, 275 plants had been domesticated in 2008.Some DH regeneration plants could transform into leaf mutants. From 2006 to 2007, 8 wrinkledness leaf mutants were gotten. The highest rate of getting mutants was 2.29 %. 2 mutant plants had gotten seeds. The leaf mutants mainly showed wrinkledness in the surface of preceding leaves at early stage. Then the wrinkle leaves could transfer to normal ones and some also keep the wrinkledness later with their growth.

结果表明,CGMCC№2553具有胚胎发生能力,2006年获得172株DH再生植株,2007年获得230株,2008年驯化275株;定植到日光温室中的DH再生植株有些会转变为叶片突变体,2006~2007年共获得8株叶片皱缩突变体,最高获得率为2.29%;目前已获得2份突变体的自交种子;叶片突变体主要表现:生长初期叶片表面皱缩呈瘤状,随着生长的进行,一些皱缩的叶片转变为正常,有的则依然皱缩。

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