查询词典 mutant

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant, which were called the wild tue strain group and the mutant stain group,respectively. The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.The induction of apoptosis was determined by flow cytometry.

将幽门螺杆菌野生株与cagA基因缺失突变株与BGC823细胞共培养，分别在6，12，24和48h观察细胞形态学变化，采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性，流式细胞仪检测细胞凋亡情况。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant,which were called the wild tue strain group and the mutant stain group,respectively.The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.The induction of apoptosis was determined by flow cytometry.

将幽门螺杆菌野生株和cagA基因缺失突变株和BGC823细胞共培养，分别在6，12，24和48h观察细胞形态学变化，采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性，流式细胞仪检测细胞凋亡情况。

Besides, the Tyr710Arg mutant produced two monocyclic products, achilleol A and camelliol C, and only the Tyr710Pro mutant lost the cyclase activity.

此外，在Tyr710Arg的突变株中也产生了两个单环的产物， achilleol A和camelliol C。而所有突变点中只有Tyr710Pro使氧化鲨烯环化酵素失去活性，且没有任何产物产生。

After the selection from one to three generations, several mutants were obtained. The major mutants included non-nodule mutants (18 lines) which failed to nodulate after inoculated with rhizobia for two weeks, ineffective nodulation mutants (9 lines) which had less uneven and white or transparent nodules, an abnormal leaf and flower mutant which was dwarf without stipules, abnormal flowers and less nodules, and a slender mutant with a thin stem and smaller leaves.

从种植的三代植株中，筛选到多种共生固氮根瘤的突变体，其中不结根瘤突变体18个株系，表现为接种根瘤菌两周后无根瘤形成；结无效根瘤突变体9个株系，表现为根瘤数目少且分布不均匀，根瘤呈白色，有些为半透明；花叶形态异常突变体1个株系，表现为除根瘤数目少外，植株矮小、托叶消失、花形态异常；纤细突变体1个株系，表现为除根瘤数目少外，植株变小、茎细叶小。

By comparing the amino acids sequences of SCRs of different regulators of complement activation and homologous structure units of DAF in different species, the conservative and nonconservative amino acids in DAF were determined. The possible activity sites were analysed and mutation amino acids sites to construct the mutant library were defined.3. Constructing and evaluating of yeast display mutant library on DAF scaffold.

SCRs多重序列比较及DAF突变位点的选择通过对补体调控蛋白家族成员、不同种属DAF分子的同源结构单位SCR的氨基酸序列进行多重序列比较，确定了DAF分子中的保守性氨基酸和非保守性氨基酸，并根据已有的实验结果，对DAF分子可能活性位点进行了分析，确定了构建酵母细胞表面呈现库的突变氨基酸位点。

To obtain more information of magnesium homeostasis in mitochondria, mTn-lacZ/LEU2 transposon library was transformed into mrs2 deletion mutant to screen for suppressor genes of MRS2. YMR166C, a member of mitochondrial carrier family, was identified as a suppressor gene of MRS2. Deletion of YMR166C gene can rescue the defects of mrs2 deletion mutant such as the decrease in mitochondrial magnesium concentration, Group II RNA splicing defect and growth defect on nonfermentable carbon source. For the first time we demonstrated YMR166C is involved in mitochondrial magnesium homeostasis.

为了增进对线粒体镁离子代谢调控基因的了解，利用酿酒酵母mTn-lacZ/LEU2转座子文库筛选MRS2的抑制基因，发现线粒体载体家族成员YMR166C基因的缺失可以挽救MRS2基因缺失的突变体的生长缺陷、II型内含子剪接缺陷，并可以调节线粒体镁离子浓度，首次发现YMR166C是线粒体镁代谢相关基因。

Iba 2002. Both the Arabidopsis fad5 mutant, which lacks an active chloroplast x-9 fatty acid desaturase and accumulates high levels of palmitic acid (16:0), and the fad6 mutant, which lacks an active x-6 fatty acid desaturase and accumulates high levels of palmitoleic acid (16:1) and oleic acid (18:1), have reduced levels of polyunsaturated fatty acids in the chloroplast galactolipids.

两个拟南芥突变体的叶绿体半乳糖脂含有较少的多不饱和脂肪酸：一种是拟南芥fad5突变体，它缺乏有活性的叶绿体的X-9脂肪酸去饱和酶，因而积累了高浓度的棕榈酸（ 16：0 ）；另一种是拟南芥fad6突变体，它缺乏一个有活性的 X - 6脂肪酸脱氢酶因而积累了高浓度的软脂酸（ 16：1 ）和油酸（ 18：1 ）。

In the present study, the autotetraploid rice IR36-4X was treated with ion implantation technique by nitrogen ion beams and a polyembryonic seedling mutant (named as the IR36-Shuang) was identified in M2 generation. The mutant line was systematically investigated about the location of seedling in polyembryonic seedling, and the cytological initiation of the additional embryo in the 1R36-Shuang was observed and determined.

以低能氮离子束为诱变源，通过对同源四倍体水稻品系&IR36-4X&进行离子注入后在其第2代群体内筛选得到了1株具有多胚苗性状特征的突变株（IR36-双），对该突变株后代的多胚苗形态特征及其多胚来源进行了研究。

Our study displayed that HmgD overexpression mutant died as late third instar larvae and malformed pupae during the larvae-prepupa transition,suggests that overexpression of HmgD has effects rather on the third instar larvae stage than on the first and second instar larvae from the mutant could not pupate.

结果表明，HmgD广泛过量表达突变体幼虫在一龄和二龄期发育正常，而在三龄幼虫化蛹时发育异常，发育停滞在三龄幼虫末期至预蛹的阶段，即HmgD过量表达的幼虫不能正常化蛹。

Amino acid mutant site A43V locates in N-terminal headpiece of represser protein which binds specifically to DNAand amino acids mutant sites S93L, S102N, G315S, Q180R, M2491, I283T all locate in the core region of represser protein which binds to IPTG.

氨基酸突变位点A43V位于阻遏蛋白氨基末端，是阻遏蛋白和操纵基因的结合区；S93L、S102N、G315S、Q180R、M249I、I283T均位于阻遏蛋白核心区，即阻遏蛋白和IPTG结合区。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。