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Xanthus DK1622 by PCR, and inserted the fragment into a site upstream of lacZ, resulting in the recombinant plasmid pZCY13. The plasmid pZCY13 was transformed by electroporation to DK1622, producing a mutant ZC16-18 ?

从黄色粘球菌DK1622基因组上PCR扩增MXAN1334基因内部部分片段，插入载体pZCY11上lacZ基因的上游，构建重组质粒pZCY13，将其转入DK622菌株，获得MXAN1334基因插入失活突变株ZC16-18。

And 3 productive mutant strains were screened from the 8 mutants by solid fermentation in 250mL Erlenmeyer flask after 3 times subculture.

将这 8 株变异株连续传代 3 次，并将 1，2，3 三代变异株进行三角瓶固体发酵，筛选出3 株正变株。

Prof. Charles Francis Xavier: Anonymity is the mutant's first defense against the world's hostility.

匿名是变态反对世界对其战争的第一道防御。

LKB1, the main upstream AMPK kinase in peripheral tissues, was redistributed from the nucleus into the cytoplasm of cells treated with SU6656 and in cells expressing a kinase-deficient, but not a constitutively kinase-active, Fyn mutant.

巴斯特研究组发现，缺乏Fyn激酶的老鼠燃烧脂肪酸更多，消耗能量更多，也越来越瘦。

The auction will feature exclusive character-based toys and collector items celebrating signature anniversaries for Barbie, GI Joe, SpongeBob Square Pants and the Teenage Mutant Ninja Turtles.

拍卖将采用专有的基于字符的玩具和藏品庆祝芭比娃娃，胃肠乔，棉球广场裤子和忍者神龟签名纪念日。

Molecular cloning and expression of FKN/CX3CR1 and their mutated counterpartsTo study the evolutionary rule in function from Old World Monkey to Homonid and the role of negative selection sites, possible Darwinian positive selection sites and deletion mutation, full-length FKN/CX3CR1 and mutant gene were obtained by overlapping extension PCR and site-directed mutagenesis.

FKN／CX3CR1分子及突变子的基因克隆和表达克隆旧大陆猴和人猿超科7个物种FKN、CX3CR1全基因及其突变体并进行体外表达，为后续观察FKN／CX3CR1在高级灵长类物种进化过程中免疫学功能的演变并在功能上验证FKN／CX3CR1协同进化以及研究上述位点对FKN／CX3CR1介导的免疫学功能的影响奠定分子生物学基础。

Interrogation of one of the mutant terrorists from the Liberty Island incident.

审问一个参与自由女神岛事件的变种人恐怖分子

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。