查询词典 mutant

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

ASR1 is a gene from early auxin-responsive GH3 gene family, and the protein encoded by this gene is overexpressed in the mutant, which results in the following morphological phenotypes including dwarf, epinastic rosette leaves, and restrained lateral root formation, etc. Meanwhile, ASR1 may modify the plant defense signaling molecule salicylic acid in vivo, and therefore asr1 mutant is more susceptible to the infection from the pathogen Pseudomonas syringae.

ASR1属于生长素相关的GH3基因家族，它编码的蛋白质在突变体内过量表达，并造成植株矮化，莲座叶片向下卷曲，侧根发生受到抑制等形态表型的改变，同时，ASR1可能还参与了植物体对抗病信号分子水杨酸的调控或修饰过程，并使asr1突变体对病原菌丁香假单胞杆菌的侵染更敏感。

In fact, any factor which interfere the formation of the hexameric IL-6 receptor complex might inhibit or antagonize the effect of IL-6. These factors include the IL-6 mutant, sIL-6Rα mutant and soluble gp130, as well as their antibodies.

事实上，任何能干扰IL-6信号转导中异六聚体复合物形成的因素都可能抑制或拮抗IL-6的作用，其中包括IL-6的突变体分子、sIL-6Rα的突变体分子和可溶性gp130分子以及它们的抗体。

Conbining previous results with our results, we conclude that the soluble gp130 mutant containing CBD can antagonize the biological activities of IL-6 by competitively binding IL-6/IL-6Rα with wild-type gp130, and the membraneanchored mutant could transduce IL-6 signal, but its efficiancy was much lower than that of wild-type gp130, therefore, we thought the Ig-like module also played an important role in IL-6 signal transduction by involving in the formation of a hexameric IL-6 receptor complex.

结合以前的研究结果，我们认为只含胞外CBD区的gp130突变体通过与膜结合型gp130竟争结合IL-6／IL-6Rα复合物而具有拮抗IL-6功能的作用；而只含胞外CBD区的gp130突变体，尽管仍能传导IL-6信号，但其效率远低于野生型gp130，因此，我们认为gp130的Ig样区在IL-6转导中也起着十分重要的作用。

Mutant libraries created by saturation mutagenesis of each single amino acid site D168, A225, K434 and E435 were screened to further improve the capability of cytochrome P450 BM-3(A74G/F87V/L188Q) mutant from Bacillus Megaterium which can hydroxylate indole into indigo. Results showed that D168, K434 and E435 are located at the functional domain of P450 BM-3 protein while A225 is sited at the unfunctional domain.

为了进一步获得具有更高活力的细胞色素P450 BM-3(F87V/A74G/L188Q)突变酶，利用饱和突变技术对该突变酶的4个氨基酸位点D168、A225、K434、E435分别进行单一的随机突变，通过对其羟基化吲哚生成靛蓝的催化性能表征，发现P450 BM-3氨基酸残基的168、434和435位均位于蛋白功能区域，而225位则位于非功能区域。

But it is also difficult to identify a mutant related to Tos17 because there are multiple copies of Tos17 in a mutant and only about 10 percent of mutants are from Tos17 insertion. Theoretically, Ac/DS two-element system is regarded as a best approach for rice insertional mutagenesis.

Tos17是目前应用最为成功的反转录转座子，但多拷贝的插入使突变体的表型鉴定和分子鉴定较为困难，因为只有10%左右的突变性状是由Tos 17插入引起的。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant,which were called the wild tue strain group and the mutant stain group,respectively.

目的 观察幽门螺杆菌野生株与cagA基因同源缺失突变株对人胃癌细胞株BGC823的细胞增殖和雕亡的影响，探讨与Hp毒力基因cagA相关的细胞水平的毒性效应。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant,which were called the wild tue strain group and the mutant stain group,respectively.

目的 观察幽门螺杆菌野生株与cagA基因同源缺失突变株对人胃癌细胞株BGC823的细胞增殖和凋亡的影响，探讨与Hp毒力基因cagA相关的细胞水平的毒性效应。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant,which were called the wild tue strain group and the mutant stain group,respectively.

结果 野生株和突变株Hp攻击BGC823细胞6h，即可引起细胞的形态学变化，48h时均可观察到细胞形态呈分散及蜂鸟样改变。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant,which were called the wild tue strain group and the mutant stain group,respectively.

方法将幽门螺杆菌野生株与cagA基因缺失突变株与BGC823细胞共培养，分别在6，12，24和48h观察细胞形态学变化，采用四甲基偶氮幽门螺杆菌；CagA；细胞增殖；细胞凋亡

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。