查询词典 mutant

The mutant △RSD-9 K141D was subcloned into the pGEX-6p-1 expression vector and the △RSD-9 protein was purified by GST affinity chromatography and digested by Prescission Protease to cut the GST domain. Next, to further verify the intracellular interaction of RSD-9/△RSD-9 and rtSH3p13 proteins, laser confocalization study and co-immunoprecipitation method were used.

随后，我们又运用蛋白质在细胞内的荧光共定位实验和免疫共沉淀实验，在细胞内相互作用水平上，证明了RSD-9蛋白/△RSD-9蛋白都与rtSH3p13蛋白在胞内相互作用。

To study furderly the activity of CNTF mutant designed by computer molecular modling,this study used the methods of dissociated cultures of chick dorsal root ganglion、TF-1 prolification and the normal mice′weight loss tests.

为了进一步研究我室应用计算机分子模拟设计并表达纯化的睫状神经营养因子突变体蛋白的生物学活性，分别采用鸡胚背根神经节无血清培养法、TF-1细胞增殖法、正常小鼠减重法对其活性进行研究。

The results indicated that the mutant protein promoted the survival of dorsal root ganglion、induced TF-1 prolification and made the normal mice lose weight,decrease appetite and reduce fat index.The weight loss effect was dependant with its administration dosage,ED50 was 150.986?

结果是突变体蛋白能促进鸡胚背根神经节的生长；促进TF-1细胞增殖，MTT测定法表明突变体蛋白与国际参考品相比，比活不低于2.0×106U/mg；使正常小鼠的体重减轻，摄食量减少，脂肪指数下降，并且体重的减轻与突变体蛋白的给药剂量呈现良好的剂量依赖关系，其ED50为：150.986？

Methods Seventy-four strains of Salmonella enterica serovar enteritidis (S. enteritidis), S. pullorum and S, gallinarum isolated from chickens were determined for biofilm using crystal violet staining, and the strain C050041 with well biofilm formation was chosen to construct a mutant library using transposon mutagenesis.

利用结晶紫染色定量法对74株鸡源的肠炎、鸡白痢和鸡伤寒沙门氏菌进行生物膜测定，选择生物膜生长较好的肠炎沙门氏菌C050041，采用转座子随机插入法构建突变株库。

Coli lpdA mutant has the potential to produce pyruvic acid from xylose and mannose.

这些结果对构建以大肠杆菌为母体的模式&细胞工厂&有参考价值。

When cells undergo programmed cell death, cell corpses adopt a refractile and disc like structure. Using this morphological change as a cell death marker, we analyzed the death of the first 13 cells in the AB cell lineage of wild type and cnx-1; crt-1 mutant embryos.

进一步以4D摄影分析线虫AB世系细胞中最早死亡的13颗细胞之相对死亡时间和细胞尸体持续的时间长短的结果则显示，此死亡细胞数量提高的现象应是源於吞噬步骤发生问题而非细胞死亡执行上的问题。

To investigate the cellular significance of targeting of PKCα to mitochondria by PICK1, REH cells clones stably expressing heterologous wild type and the non-interacting mutant PICK1 are selected.

为要探讨PICK1 将PKCα带到线体上对细胞功能的重要性为何，将PICK1及失去和PKCα交互作用能的PICK1 蛋白过表现於母巴球细胞REH。

Cells were immediately fixed and processed for immuno?uorescence and tested for translocation ofβ-catenin.β-catenin and target genes of Wnt/β-catenin signaling COX-2, cyclinD1, c-fos, c-Jun mRNA expression were examined by RT-PCR. Saos-2 osteoblastic cells were co-transfected with a wild type or mutant Tcf luciferase reporter gene and Renilla luciferase plasmid. Posttransfection cells were subjected to 1 hour of 12% elongation.

作用1h、2h和4h后，用免疫荧光的方法检测β-catenin的表达和在细胞上定位的变化；用半定量RT-PCR检测β-catenin mRNA表达的变化；用质粒转染的方法检测荧光素酶报告基因Tcf转录的情况；用半定量RT-PCR检测Wnt/β-catenin通路下游基因COX-2，cyclinD1， c-fos， c-Jun mRNA表达的情况；用免疫共沉淀的方法检测β-catenin和E-Cadherin的结合情况。

OBJECTIVE: To construct a novel adenoviral eukaryotic expression vector that can co-express mutant hypoxia-inducible factor-1 alpha (HIF-1α) target protein and humanized Renilla reniformis green fluorescent protein reporter molecule under normoxic conditions.

目的：构建能够同时表达突变型低氧诱导因子1α(hypoxia inducible factor 1 alpha，HIF-1α)目的蛋白和人源化绿色荧光蛋白(human renilla reniformis green fluorescent protein，hrGFP)报告分子的新型腺病毒真核细胞表达载体。

The replicons were detected and characterized by RT-FUR. IFA. Renilla Luciferase assay system and real time RT-FUR. respectively. Results It was shown that SLB2 mutant did out significantly affect the translation of the input RNA.

将突变体（包括相关的阳性和阴性对照复制子）分别线性化并体外转录成RNA后，取等量各转录体RNA，以脂质体法分别转染BHK-21细胞。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。