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Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

An important part of this study was to deal with a avirulence gene mutant, MS220, which was obtained from the pool of transformants generated by SacⅠ mediated transformation and changed its avirulence into virulence on rice cultivar Aichiasahi (carrying resistance gene Pi-a).

本研究的一个重要组成部分是作者对从SacⅠ介导的转化群体中筛选得到一株对水稻品种爱知旭特异性发生改变了的突变体MS220进行分子遗传学分析。

The Caflo8/Caflo8 mutant is avirulent in a mouse model of systemic infection, and the

在小鼠系统感染试验中，Caflo8缺失株几乎没有毒性

Objective To study the role of mLT63, an avirulent mutant of heat-liable E. coli enterotoxin, as an adjuvant in intranasal immunization and compare with that of CpG-ODN.

目的 研究大肠杆菌不耐热肠毒素无毒突变体mLT63和CpG-ODN鼻内免疫对抗原的佐剂效应。

A mutant of Impations balsamina was obtained after space induction, and its traits and meiosis were investigated.

本文报道了种子经神舟 4号飞船搭载的凤仙花突变植株的性状及减数分裂过程的变化。

Several SP2 mutant plants were obtained from induced Impatiens balsamina L.seeds by space flight,and their blades were investigated in this paper.The writer calculated the K-S statistics and the levene statistics,also made use of SPSS 11.5 to have the independent-samples t test and the univariate in the prerequisite of some fundamental presumptions.

对航天诱变凤仙花SP2代若干单株的叶片长度和宽度进行了统计分析，对试验数据进行了柯尔莫哥罗夫-斯米尔诺夫检验，计算了样本的列文统计量，并采用Spss 11.5统计分析软件检验了基本假设的满足条件，进行了独立样本t检验。

An assay of polar auxin transport showed that, in comparison with wild type plants, the basipetal transport activity was greatly reduced in the stem of li mutant.

形态解剖观察发现li突变体茎的形成层和韧皮部分化发育不完全，这与其生长素极性运输能力下降是一致的。

This paper focused on the breeding of an L-lactic acid producing bacteria Lactobacillus easei YBQ1-1, studying the characteristics, medium components, pH and metabolic network of the mutant YBQH2-14 obtained after being selected.Using UV irradiation, L.casei YBQH2-14 was selected in the different plate media such as the medium with high concentration of glucose, lactic acid , CaCO3 -Bromocresol purple and KMnO4 -KBr.

本文主要对L-乳酸生产菌株(Lactobacillus casei YBQ1-1)进行了诱变选育，并研究了突变株YBQH2-14发酵条件对生产L-乳酸的影响，构建了L.caseiYBQH2-14的代谢网络模型，分析了分批发酵不同阶段的代谢通量，同时研究了L-乳酸的应用——黑麦麸皮粉发酵。

In this paper, an efficient and easy biological screening method based on the sensitivity to lovastatin of Neurospora crassa was built to deal with the mutant strain rudely by UV mutation. A high-level strain was screened in the shaking flask culture incorporating with TLC and HPLC. A comparison between this two methods also was originally discussed while identifying dyeing using bromophenol blue as the better treatment of TLC, and offering a kind of simple, practical, fast and economical determination qualitatively and quantitatively.

本文有鉴于此，通过紫外诱变处理，并基于粗糙脉孢酶对洛伐他汀的生物敏感性，对诱变菌株进行了初筛；同时，采用摇瓶培养，结合TLC和HPLC的分析方法，复筛出一株高产菌株；对复筛过程两种分析方法分别进行了初步探讨，比较其优劣，确定TLC法经溴酚兰显色剂显色处理为较佳方式；从而，建立了一套简单、实用、快速、经济且有效的洛伐他汀生产菌诱变筛选方法。

Additionally, the binding modes of the three mutant camelid VHH antibodies and α-amylase (denoted as T04, T05 and T06) provided by CAPRI (Critical Assessment of Prediction of Interactions) meeting were predicted using the complex type-dependent docking algorithm.

另外，采用该分子对接方法，我们对2002年CAPRI(Critical Assessment ofPrediction of Interactions)学术大会提供的三种不同突变形式的骆驼VHH抗体和胰腺α-淀粉酶分子之间相互作用与识别进行了预测（分别记作T04、T05和T06）。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。