查询词典 mutant

Longitudinal root sections of the wild type and the gls mutant cultured in nutrient solution for 4 days showed that biosynthesis and distribution of amyloplast in the columella cells of gls were normal as compared with the wild type, suggesting that root gravitropism loss in gls mutant is not caused by the defect in amyloplast sedimentation, but possibly by the disturbance of auxin transport and distributation.

4天龄的根系纵切片结果显示：突变体gls根尖结构完整，根尖柱细胞内淀粉粒的生成和分布正常，表明gls突变体根系向地性的缺失不是由于淀粉体的原因，而可能通过影响生长素运输和分布造成。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

Data on mutant strains includes detailed information on affected gene, mutant allele and genetic background (DNA origin, gene targeted, host and backcross strain background).

突变系的数据包括对影响的基因、突变等位基因和遗传背景(DNA起源，基因靶向，宿主和回交系背景)的详细的信息。

We isolate the putative NO-sensitive bushiness mutant and characterize the mutant nob by T-DNA inserted mutation and root inhibition effect of the NO donor , sodium-nitroprussiate , in Arabidopsis thaliana.

本实验以拟南芥为材料，利用T-DNA插入诱变和NO胁迫选择的方法，筛选到一种对NO敏感且叶片丛生的突变体nob。

Quantitative RT-PCR results showed that HTF1 is involved in the conidiation pathway,Htf1 mutant is not de-repressed in conidiophore development under non-conducive conditions.The observed phenotype of enhanced conidiophore development in the htf1 mutant may result from its defect in conidium development.It is possible that some feedback signal from conidia may inhibit further conidiophore development in M.grisea.

定量RT-PCR的结果也表明HTF1参与产孢信号途径。htf1突变体在不利于产孢条件下并没有去抑制分生孢子梗的发育，因此htf1突变体产生比野生型菌株数量更多的分生孢子梗可能是由于其无法形成分生孢子的原因导致的，这也似乎预示了在稻瘟病菌中很有可能存在一些来自分生孢子的反馈信号以进一步抑制分生孢子梗的发育。

When grown in the light and with nutrition as the limiting factor, the wild type was less influenced due to its capability of photosynthesis and quickly became predominant over the mutant that has no photosynthetic apparatus. However, when grown heterotrophically in the dark, the mutant remained less competitive than the wild type, showing that the euglenoid proplastids play a role in competition.

在光照情况下，当营养物质成为限制因子时，由于具有光合能力野生型生长受影响较小，在与没有光合器的突变株的竞争中很快地显示出优势；在黑暗中二者均只能以异养方式生长，但突变株仍然处于竞争弱势，显示原质体结构对于生存竞争的作用。

ResultsNo significant differences were found in the cell morphology, growth rate or heterocyst development between the deletion mutant and the wild type strain. But the mutant was more sensitive to Na+-salt under salt-stress.

敲除该基因后，发现缺失突变体的形态、生长速度及异形胞发育与野生型无明显差异，但在盐胁迫条件下，突变体比野生型对钠盐更敏感。

In the fin219-null mutant background, GUS staining can be detected in the hydathode of cotyledons, and the guard cells around hydathodes. GUS staining are also found in the base of trichomes, hypocotyls and roots. In contrast, in the phyA mutant background, GUS-FIN219 was not expressed in the hypocotyls.

结果发现转殖在fin219-null突变株背景下，FIN219会特别表现在植株叶缘出水孔与附近的保卫细胞及毛茸基部、初生叶、下胚轴、根部等处，然而在phyA缺失的时候植株幼苗的FIN219则不会在下胚轴表现。

Results The GRK5 protein level in Parkinson's disease α-synuclein transgenic mice was increased to different degrees compared with that in non-transgenic littermate control mice, and the protein and mRNA expressions of GRK5 in 9-month old A53T mutant mice were higher than those in NLC mice, but there were no significant changes in the 3 month and 6 month old A53T mutant mice.

结果 各组帕金森病α-synuclein转基因小鼠与阴性对照小鼠相比，GRK5蛋白表达水平均有不同程度的增加，并且随着转入的h-α-syn蛋白表达水平的高低而有所变化。3月龄和6月龄帕金森病转基因模型小鼠与同月龄阴性对照组小鼠相比，GRK5的mRNA和蛋白水平没有变化；而9月龄帕金森病转基因模型小鼠与同月龄阴性对照组小鼠相比，GRK5的mRNA和蛋白水平都有所增加。

The morphogenesis of floral organs in both srg mutant and wild-type rice Dular (Oryza sativa L.) was observed using the scanning electron microscope. The results showed that the floral primordia were differentiated under irregular pattern in srg mutant. After regular formation of lemma and palea primordia, the floral primordium of srg formed lodicule primordia inner to both lemma and palea, followed by the formation of stamens and carpel. Alternatively, floral primordia in srg were split to form two or more floral primordia. The number and position of stamens and carpels primordia were malformed.

利用扫描电子显微镜观察野生型水稻和裂颖水稻的颖花原基分化情况，结果发现，突变体水稻的幼穗在形成内、外稃原基后，有些颖花在形成浆片后并不是直接发育形成雄蕊原基，而是在外稃或内稃原基内侧又分化出浆片原基；有些颖花原基从中间缢裂，缢裂的颖花原基又分别形成类稃片的结构，颖花发育后期，6枚雄蕊发育畸形，并且观察到不同数目的雄蕊。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。