查询词典 mutant

To make comparison of adaptability of the plastid-less mutant and the wild type strain, we studied their growth characters under different culture conditions, found that the difference of their growth in a medium rich in organic substances are hardly detectable and that the loss of plastid exerts only a weak effect, if any, on the heterotrophic growth.

为比较完全失去质体结构的突变株和野生型的适应力，我们对二者在不同条件下的生长特性进行了研究，结果发现野生型与突变株的生长在有机质丰富的培养液中差别微弱，质体的丢失并不产生显著影响。

Results:Mutant CD59 cDNAs subcloned into the mammalian expression vector PLATER and transfected CHO together with the pcDNA,which confered resistance to G418. The positive clones were tested by FIH.

结果：运用阳离子脂质体导入法将重组pALTER质粒与pcDNA3质粒共转染CHO，成功筛选出的阳性克隆经FIH证明突变CD59可在CHO细胞表达。

In addition, exposure to plumbum significantly repressed the LTM at 18, 36, and 48 h after training in both wild-type N2 and ncs-1 mutant animals.

而铅暴露可以显著抑制训练后18、36和48 h野生型和 ncs-1突变体动物在不同时间点下的IT百分数。

Francis expressed truncated mutant pokeweed antiviral protein gene in Pichia pastoris. The yield reached 10-12mg/L.

Francis等在巴斯德毕赤酵母中表达了信号肽和毒性区缺失的PAP基因，使其表达量达到了10-12mg/L。

Pokeweed antiviral protein belongs to Ribosome-inactive Proteins . PAP has potent antiviral activity against many plant virus. pTI was used as the template to amplify the deleted mutant PAP gene (N-deleted and N-, C-deleted) by PCR and then the two genes were cloned into expression vector.

美洲商陆抗病毒蛋白是一类能很好的抑制植物病毒的蛋白，很早就有报道低剂量的商陆叶片粗粉末就能明显的抑制TMV在菜豆叶片上局部枯斑的形成，PAP缓释剂也己用于病毒病的防治工作。

The total RNA was isolated from pokeweed leaves using the method of guanidine isothiocyante and used as template for amplification of the deleted mutant pokeweed antiviral protein gene by RT-PCR and then the amplified fragment was cloned into secreted expression pPIC9K vector to form pPIC9K-p, then the vector was transferred into Pachia pastoris GS115 strain.

从美洲商陆叶片中通过异硫氰酸胍法提取出了总RNA，经RT-PCR扩增出缺失突变型抗病毒蛋白PAP基因，将该基因克隆于分泌型真核表达载体pPIC9K，然后导入毕赤酵母菌株GS115细胞。

PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶，切下表达的目的蛋白，冰浴研磨后用磷酸缓冲液溶解，再加入等体积的福氏不完全佐剂，乳化完全后，免疫家兔，制备抗PAP蛋白的多克隆抗体，间接ELISA法测定所制备的抗血清的效价为1：1000。

It was showed that 9 mutants were phenotype male sterility mutant of 'pollyanna'.

它们表现出与'pollyanna'差异的多态性位点有 7～ 1 8个，表明它们为'pollyanna'的基因型突变体。

Strictly speaking, there shouldn't be such thing as a polyploid mutant.

严格来说，应该没有如一个倍数体突变异种的事物。

Using TaKaRa LA PCR? in vitro Clonging Kit, a 2.3kb DNA fragment from the mutant was amplified and identified. Sequence analysis indicated that the fragment was composed of a complete proE gene and a partial proA gene.

根据枯草芽孢杆菌93151野生菌株proB基因的序列设计并合成引物，利用TaKaRa LA PCR~ in vitro Clonging Kit试剂盒，克隆突变菌株的proBA基因，得到一个约2.3kb的DNA片段，序列分析表明该片段包含一个完整的proB基因和不完整的proA基因。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。