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Another mutant named UV20' was also gained whose main end metabo lites were butyric acid and acetic acid.

另一个突变命名uv20 '也上涨，其主要最终代谢lites被丁酸和乙酸。

The effect of a Lactobacillus casein mutant correlated conditions of immobilization on the biosynthesis of CLA was studied.

关于一株干酪乳杆菌突变株的固定化相关条件对共轭亚油酸生物合成的影响进行了研究，得出了最佳培养条件，从而提高了共轭亚油酸生物合成的产量。

The highest enzyme producer alkalophilic Bacillus sp SHY8-1 among the inductive strains was mutagenized several times, by adding enzyme production repression aegents into screen medium, combined with natural seperation and pressure selection, finally a CMCase-hyperproductive mutant SHY8-1-25 (partitally resistant to catabolite) was obtained, with the yield of CMCase raised to 13-16u/ml, maximally reached 18-20u/ml, while its parent strain 0.654u/ml.

对诱导型产酶菌-嗜碱芽孢杆菌SHY8-1进行了多次诱变改良，采用添加产酶阻遏剂的平板作为筛子，结合反复的自然分离、压力选择，最终成功地获得了高产变异株SHY8-1-25(已部分解除分解代谢阻遏)，产酶量由0.654u/ml提高到13-16u/ml，最高达到18-20u/ml，讨论了诱变机理策略，考察了碳、氮源等对产酶的影响，对培养基和摇瓶培养条件进行了优化。

Mutant PF 11 was tentatively identified as gram positive Bacillus cereus , which has not been reported in detail.

经初步鉴定，突变株PF-11为以前未见报导的革兰氏阳性Bacillus cercus菌。

In addition, complementation of minE gene in minE mutant strain, approximately 41% bacterial cell length was restored.

此外，在minE的互补株中，有41%的菌体长度能回复成野生株的形态。

Because positive clones utilizing nitrate or nitrite as sole nitrogen source werentdirectly isolated from the genomic library, random miniTn5 insertion mutant library wasconstructed via biparental conjugational transfer between E.

由于不能从文库直接筛选到利用硝酸盐的阳性克隆，因此，通过Tn5插入突变构建了随机插入突变库，采用E。

Up to now, analysis on salt cress physiology , biochemistry and gene expression, and the construction of different cDNA libraries and mutant libraries have made salt cress a valuable model for the study of salt tolerance.

目前，对盐芥生理生化的研究、基因表达的研究，以及各种cDNA文库、突变体库的建立，已经使盐芥成为一种非常有价值的耐盐模式系统。

In this research, the ~ and F2 of the crosses between a natural mutant 慪34?with super-minute grain and 慪38?with super-large grain,慡huhui 881擲huhui 527?with middle grain size were carried out to study the inheritance of the grain shape (grain length, grain width, grain thickness and grain length/width ratio) and 1000-grain weight. The main results are summarized as below:? The F1 grain length, grain width, grain thickness and 1000-grain weight of three crosses were lay between the two parents and tended to Y34, which indicated that those grain traits were all governed by the dominant effect of Y34 and influenced by both female and male parents. The differences of F1 grain length, grain width, grain thickness and 1000-grain weight of positive and negative crosses between Y38 and Y34 indicated the existence of cytogene effects.? The broad heritabilities of major grain traits were calculated.

本研究利用一份水稻极小粒自然突变材料Y34与一份水稻极大粒材料Y38、两份常规籽粒大小材料蜀恢881、蜀恢527的杂交F_1及F_2，对主要粒形性状（粒长、粒宽、粒厚、长宽比）及千粒重进行了遗传研究，根据遗传研究的结果利用微卫星标记结合F_2群分法对控制Y34短粒性状基因进行了分子标记定位，主要结果如下：●各组合F_1粒长、粒宽、粒厚和千粒重介于双亲之间且明显偏向于小值亲本 Y34，这表明粒长、粒宽、粒厚和千粒重均主要受小值亲本显性基因的控制并同时也受大值亲本核基因的影响。Y34与Y38正反交F_1在粒长、粒宽、粒厚、千粒重等性状上存在差异，表明存在细胞质效应。

Essential amino acid residues in diphtheria toxin catalytic domain were determined by quantum chemistry calculation and the enzymatic activities of mutant in the active-site were tested in order to provide high effective cytotoxin for target anticancer drugs.

通过量子化学计算确定白喉毒素分子催化区活性中心的关键氨基酸残基，评价其取代后的酶活性的改变，为导向性抗癌药物研究提供高效杀伤细胞工具。

First, in silico modeling of the mutant protein predicted destabilization of the SH2 domain.

首先，突变蛋白质硅模拟预测了SH2结构域的不稳定性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。